Mounting A S Ample; Finding T He S Ample B Y E Ye - Leica SP5 confocal User Manual

Mounting a s ample
Ensure the correct stage insert is mounted (see facility staff if
1.
unsure)
Remember to invert your slide so that the cover glass is the closest
2.
surface to the lens
Select the objective lens either using the two buttons behind the right focus
3.
wheel or in the software within the Acquire tab then select the Objective text to
see a drop down list of lenses
Use the joystick to move the stage if necessary. Change the XY speed
4.
of the stage by pressing the black buttons on the left side of the joystick
base. The black buttons on the right change the speed in Z.
Finding t he s ample b y e ye
1. Choose a fluorescence filter cube via the buttons on the front of the microscope.
Push the shutter button to toggle open/close shutter.
I3 = FITC
N2.1 = TxRd
A = DAPI
Fluorescence
shutter
BF intensity
(Controller on
LHS of microscope
Z position
2. Once the sample is in focus you can record this Z
position by holding the two outer Set Z buttons on the
right side of the microscope. This sets this Z position to
5
Z
X
Y
Filters
Observation
mode BF/FL
Focus speed
(course/fine)