GE ÄKTA start Maintenance Manual page 9

3. Note the current temperature of the calibration
solution in the conductivity flow cell as displayed in the
Cell temperature field.
4. In the Calibration screen, enter the conductivity value
at current temperature in the Set conductivity field
and then tap Calibrate to carry out the calibration.
Note:
• If a certified conductivity standard solution is used, use
the supplied theoretical conductivity value corresponding
to the temperature in question.
• If a manually prepared 1.0 M NaCl calibration solution
is used, get the conductivity value at the current
temperature from the graph for conductivity of 1.0 M
NaCl as a function of temperature presented in the ÄKTA
start Maintenance Manual.
System performance test
System performance test is performed to make sure
the system is performing within acceptable limits. It is
recommended to run this method during installation of
the instrument or after replacement of modules such
as Pump, UV, Conductivity or Valves. For a detailed
description of the test, refer to ÄKTA start Operating
Instructions, section 5.4 System performance.
Required solutions
• Buffer A: DM water
• Buffer B: 1.0% Acetone, 1.0 M NaCl
• Sample: 1.0% Acetone, 1.0 M NaCl (Buffer B)
Checklist
• Calibration of all the modules: Pressure sensor, Pump,
UV and Conductivity.
• Set the conductivity reference temperature to 20 °C.
• No column should be present in the flow path.
• Immerse Buffer port A inlet in Buffer A (DM water).
• Immerse Buffer port B inlet in Buffer B (1.0% Acetone,
1.0 M NaCl).
• Sample valve inlet immersed in sample (1.0% Acetone,
1.0 M NaCl).
• Make sure that 1 ml Sample loop is filled with sample
(1.0% Acetone, 1.0 M NaCl).
• Make sure that 2 m of 0.5 mm ID tubing is connected to
the Outlet valve at Waste position.
• When performing System performance without Fraction
collector, make sure the outlet valve fractionation tubing
is inserted into a pre-weighed beaker.
• When performing System performance with Fraction
collector, make sure the outlet valve fractionation tubing
is connected to Fraction collector with at least 5 pre-
weighed tubes.
• Make sure that the system is prefilled with DM water.
ÄKTA start Maintenance Cue Card, 29-0240-43 AC
• Make sure to take note of all the required observations
by recording parameters while the performance run
is in progress. Enter the observed values in the ÄKTA
start report template available in ÄKTA start Operating
Instructions, section 10 Appendix.
Operation from the Instrument Display
Prepare system
System performance
Acceptance criteria
Time Activity
(min)
0 Pump wash
1 1 ml/min, 0 %B, flow
through Outlet valve,
Waste position
 Repeat UV Auto Zero
3 5 ml/min
4 1 ml/min, Sample
valve, Sample position
7 1 ml/min, Sample
valve, Buffer position
10 Request switch Injec-
tion valve to Inject
position
13 Request switch Injec-
tion valve back to
Load position
15 Start gradient,
0-100 %B in 10 min,
start fractionation/
collection
19 End fractionation
1
0 End collection

5 End gradient, stay at
100 %B
8 50% B
36 0% B (re-equilibrate)
41 End
1 with Fraction collector  without Fraction collector
ÄKTA start
Method run
method
Run End
Exit
Check Aproved
interval
Wash valve Mobile phase
position out through
Waste
Back pressure =< 0.05 MPa
Back pressure 0.06 - 0.2 MPa
UV level ± 10 mAU
Cond. level ± 1 mS/cm
Max UV level 300-380 mAU
Max Cond. level 65 - 95 mS/cm
Max UV level 300-380 mAU
Max Cond. level 65 - 95 mS/cm
Weigh fraction no 0.8 - 1.2 g
2, 3 and 4
Max diff. between 0.1 g
fractions
Weigh beaker 4.2 - 5.8 g
Gradient Straight, no
negative dips.
Gradient level 45 - 55 %B
3
Check all connec- No leakage
tions for leakage
3 UV 50% B / UV 100% B
9