Storage Of The Instrument; Safety Precautions - GE ÄKTA start Maintenance Manual

Instruction
1. Check that no run is in progress.
2. Switch off ÄKTA start and disconnect the Frac30 Cable.
3. Remove the collection tubes from the Bowl assembly.
4. Gently move the Dispenser arm counterclockwise to
the end position (1).
5. Push the drive assembly and hold it at the retracted
position (2).
6. Lift and remove the Bowl assembly (3).
7. Wash the Bowl under a water tap. Use a mild detergent
if required, and rinse thoroughly with water.
8. Wipe the surface with a damp cloth. Wipe off stains
using water.
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9. Let the Bowl assembly dry completely before re-
assembling.
10. Re-assemble the Bowl assembly on to the Base unit:
• Orient the Bowl to match the aligning groove and the
aligning features on the bowl holder (1)
• Lower the bowl assembly on to the Base unit (2) and
push the drive assembly to allow the Bowl assembly to
get in position (3)
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ÄKTA start Maintenance Cue Card, 29-0240-43 AC

Storage of the instrument

Short term storage
If the system is not going to be used for 1-2 days, prepare
the system for short term storage.
Storage solution: DM water
Note: Before cleaning the flow path, remove the column
and re-connect the flow path.
Instruction
1. Immerse the buffers and the sample inlet tubing in DM
water.
2. Run system methods (Pump wash A & B, Washout
fractionation tubing) or run manually to flush the flow
path (including all inlets and outlets) with DM water.
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Pump at least 20 ml water through the system.
3. End the run and leave the system filled with DM water
during the storage period.
2 4. Open the pump cover after switching off the
instrument.
Long term storage
If the system is not going to be used for longer than 3 - 4
days, prepare the system for long term storage.
Storage solution: 0% ethanol (aqueous solution)
Note: Before cleaning the flow path, remove the column
and re-connect the flow path.
Instruction
1. Immerse the buffer and the sample inlet tubing in 20%
ethanol.
2. Start a manual run and pump 0 ml of 0% ethanol
through the system.
3. Edit the run and set the run parameters to clean the
sample inlet tubing and the outlet tubing for fraction
collection.
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4. End the run and leave the system filled with 20%
ethanol during the storage period.
5. Open the pump cover after switching off the
instrument.
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Safety precautions

These user instructions do not guarantee user safety - it
is the responsibility of the user's employer to ensure safe
operation and training. Training courses are available and
can be arranged by contacting your local GE Healthcare
office.
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