GE ÄKTA start Maintenance Manual page 6

Operation from the Instrument Display
Perform the following operations to run Washout
fractionation tubing.
Prepare system
Washout fractionation
Set Run parameters to
suit your requirement
Edit run
Operation from UNICORN start
Perform the following operations to run Washout
fractionation tubing.
UNICORN start
System control
System preparation
Washout fractionation
System cleaning
The System cleaning method is used to clean the
instrument flow path. The method is recommended for:
• Routine maintenance
• Preparing the system for storage
• Removing/preventing contamination (bacterial growth)
in the flow path
• Preventing carryover of buffers/samples
Cleaning solutions
• 1 M NaOH
• DM water
6
ÄKTA start
Method run
tubing
Run End
Exit
Method run
Pre defined
tubing
Instruction
1. Clean the inlets and outlets (buffer tubing, sample inlet
tubing, fractionation tubing)
2. Immerse the buffer inlet tubing (A & B) in 1 M NaOH.
3. Disconnect column from the flow path and reconnect
the tubing with a Union connector.
4. Follow the workflow below to run System cleaning.
Note:
• Do not leave NaOH in the system. Wash the flow path
with water thoroughly.
• Check the pH after water wash to ensure complete
removal of NaOH.
• It is recommended not to end the method prior to its
completion.
Operation from the Instrument Display
Prepare system
System cleaning
Follow the instructions
& tap Continue
When prompted, immerse the
buffer inlets (A & B) in DM water
Operation from UNICORN start
UNICORN start
System control
System preparation
System cleaning
ÄKTA start Maintenance Cue Card, 29-0240-43 AC
ÄKTA start
Method run
Continue
Run ended
Exit
Method run
Pre defined