Nikon Eclipse Ni-E Instruction Manual page 49

Problem
The nosepiece is attached incorrectly or
not rotated to the click stop position.
The specimen is tilted relative to the stage
One side of the field of
surface.
view (up, down, right, or
left) is not focused.
The image flows (i.e.
becomes asymmetrically
The stage is tilted.
defocused when moving
the focal point)
The substage is tilted.
The condenser is tilted.
Interpupillary adjustment has not been
performed.
Images in left and right
eyepieces are not
coincident.
Diopter adjustment has not been
performed.
Diopter adjustment has not been
performed.
Eyes become fatigued.
Brightness is inadequate.
The specimen holder is not securely-fixed
to the stage.
The specimen does not
move smoothly.
Rotating torque of the stage knob is set too
heavy.
Difficult to focus.
The focusing speed of the focus knob is
(When using ergo
not set properly.
controller and joystick)
The optical path does
not switch when the
The quadrocular tilting tube is fastened.
quadrocular tilting tube
is used.
Chapter 2
Troubleshooting
Cause
41
Measure
Attach it correctly and rotate it to the click stop
position.
(→See Chapter 1 "3 Assembly Method - 19 Attach
the nosepiece" in the "Assembly/Maintenance".)
Position the specimen in place on the stage.
(→See Chapter 2 "3 Bright/Dark-field Microscopy
Procedure - 8 Place a specimen on the stage, and
move the stage to bring the target into view" in the
"Operation".)
Attach the stage correctly.
(→See Chapter 1 "3 Assembly Method - 17 Attach
the stage" in the "Assembly/Maintenance".)
Attach the substage correctly.
(→See Chapter 1 "3 Assembly Method - 16 Attach
the substage" in the "Assembly/Maintenance".)
Attach the condenser securely.
(→See Chapter 1 "3 Assembly Method - 18 Attach
the condenser" in the "Assembly/Maintenance".)
Perform interpupillary adjustment.
(→See Chapter 2 "3 Bright/Dark-field Microscopy
Procedure - 11 Adjust the interpupillary distance" in
the "Operation".)
Perform diopter adjustment.
(→See Chapter 3 "6 Adjusting the Diopter" in the
"Operation".)
Perform diopter adjustment.
(→See Chapter 3 "6 Adjusting the Diopter" in the
"Operation".)
Adjust the brightness using the brightness
control knob or ND filters to attain a suitable
brightness.
(See Chapter 3 "3 Adjusting the Brightness of a
Diascopic Image" in the "Operation".)
Fix the holder securely.
(→See Chapter 2 "3 Bright/Dark-field Microscopy
Procedure - 8 Place a specimen on the stage, and
move the stage to bring the target into view" in the
"Operation".)
Adjust to the appropriate torque weight.
(→See Chapter 3 "5.1 Operating the Rotatable
Ceramic Coated Stage - Adjusting the knob rotation
torque" in the "Operation".)
Set to the appropriate focusing speed.
(→See Chapter 3 "20 Using the Ergo Controller"
and "21 Using the Joystick Controller" in the
"Operation".)
Unfasten it. (Remove the tightening screw for
the quadrocular tilting tube.)
(→See Chapter 1 "3 Assembly Method - 12 Attach
the quadrocular tilting tube and DSC zooming port
for quadrocular tube." in the
"Assembly/Maintenance".)