Nikon Eclipse Ni-E Instruction Manual page 48

Problem
Parts are attached incorrectly.
Movable part of manually operated unit is
not switched correctly.
Field diaphragm image is not focused on
the specimen surface.
Lack of visibility around
periphery of field of view.
Field diaphragm is stopped down too far.
Illumination is uneven
across the field of view.
Field of view is not
Incorrect combination of the objective with
visible.
the condenser.
When using objectives with 4x or less, 2-4x
auxiliary lens of universal condenser is not
in the optical path or condenser switching
such as swing-out or sliding operation is
not performed.
The lamp is attached incorrectly.
The Lens and specimen are dirty or dusty.
The specimen is upside down.
Out of focus with an
The thickness of the cover glass is
objective of high
inadequate.
magnification.
Fail-safe device for specimen damage
protection of the objective is pushed in.
Objective is attached incorrectly.
A focal deviation is high
when switching over
objectives.
Diopter adjustment has not been
performed.
The stage is attached incorrectly.
Image is not in focus
although the stage is
raised to the highest
The refocusing position is set lower than
position.
the focusing position.
Chapter 2
Troubleshooting
Cause
40
Measure
Confirm that parts (nosepiece, condenser, etc.)
are correctly attached.
(→See Chapter 1 "3 Assembly Method" in the
"Assembly/Maintenance".)
Correctly set the optical path switching knob,
nosepiece, filter cube switchover turret,
condenser turret, and slider, etc. (Move the
part until it clicks.)
Make sure the condenser is focused and
centered.
(See Chapter 3 "7 Focusing and Centering the
Condenser" in the "Operation".)
Open the field diaphragm slightly wider than
the field of view.
(→See Chapter 3 "10 Adjusting the Field
Diaphragm" in the "Operation".)
Adopt an appropriate combination.
(→See Chapter 3 "9.1 Compatibility of Condensers
with the Magnification of Objectives" in the
"Operation".)
Bring 2-4x auxiliary lens of universal
condenser in optical path.
For other condensers, swing-out or slide the
condenser.
Attach it correctly.
(→See Chapter 1 "3 Assembly Method - 6 Attach
the lamp" in the "Assembly/Maintenance".)
Clean them as appropriate.
(→See Chapter 3 "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
Turn up the cover glass and attach it to the
stage.
(→See Chapter 2 "3 Bright/Dark-field Microscopy
Procedure - 8 Place a specimen on the stage, and
move the stage to bring the target into view" in the
"Operation".)
Attach a cover glass of the specified thickness
(0.17 mm).
Some objective has a stopper to keep the
pushed in state. Turn the tip of the object to
release.
If the objective does not have a stopper, do
not pull because the tip cannot be turned. In
this case, contact your nearest Nikon
representative.
Screw the objective all the way in.
(→See Chapter 1 "3 Assembly Method - 20 Attach
the objective." in the "Assembly/Maintenance".)
Perform diopter adjustment.
(→See Chapter 3 "6 Adjusting the Diopter" in the
"Operation".)
Attach it correctly.
(→See Chapter 1 "3 Assembly Method - 17 Attach
the stage" in the "Assembly/Maintenance".)
Check and reset the set value.
(→See Chapter 3 "23.1 Setting Up the
Microscope - (6-4) Software Limit" in the
"Operation".)