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2-2. Phase-contrast (Ph) microscopy
Key point: Slide the Ph annular diaphragm slider to move the annular diaphragm with the same Ph code as the objective
into the optical path, then conduct microscopy. Note that the PhL diaphragm must be centered.
1.
Focus on the specimen using bright-field microscopy.
2.
Set the microscope for phase-contrast microscopy.
1) Move a Ph objective into the optical path.
2) Slide the Ph annular diaphragm slider to move the annular
diaphragm < with the same Ph code > as the objective
placed in the optical path in step 1) into the optical path.
3.
Center the PhL diaphragm.
1) Move the < PhL > objective into the optical path.
2) Slide the Ph annular diaphragm slider to move the < PhL >
diaphragm into the optical path.
3) Remove one eyepiece, and insert the centering telescope
using the adapter.
4) Turn the eyepiece of the "centering telescope" to bring the
annular diaphragm image into focus.
5) Turn the two "annular diaphragm centering screws" on the
condenser so that the annular diaphragm image coincides
with the phase plate image in the objective.
6) Reinstall the eyepiece to the original position.
4.
Conduct observation.
1) Remove the "NCB11 filter" on the dia-illuminator from the
optical path, and move the "GIF filter" into the optical path.
(-> To improve the contrast)
2) Move the "ND filter" on the dia-illuminator in and out of the
optical path to adjust the brightness of the view field.
Supplementary information
If precise color fidelity is not required, the brightness
can be adjusted by varying the lamp voltage using the
"brightness adjustment dial" on the left side of the
microscope.
II. Microscopy
27
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