Nikon ECLIPSE TE2000-E Instructions Manual page 24

II. Microscopy
4) Turn the "photo-mask dial" on the front side of the
microscope clockwise to remove the photo-mask from the
optical path.
5) Adjust the distance between the eyepieces to consolidate
the view fields of both eyepieces.
7. Adjust the focus.
1) Place a specimen on the stage.
2) While looking into the eyepieces, turn the "fine/coarse
focus knob" to bring the specimen into focus.
8. Center the condenser.
1) Make sure that the < 10X > objective is in the optical path.
2) Lower the "field diaphragm lever" on the dia-illuminator
until the field diaphragm image appears in the field of view.
3) Turn the "condenser focus knob" to bring the field
diaphragm image into focus.
4) Turn the two "condenser centering screws" to move the
field diaphragm image to the center of the view field.
5) Select the < 40X > objective.
6) Adjust the position of the "field diaphragm lever" on the
dia-illuminator until the size of the field diaphragm image is
about the same as that of the view field.
7) Turn the two "condenser centering screws" on the dia-
illuminator to move the field diaphragm image to the center
of the view field.
9. Conduct observation.
1) Select an objective of desired magnification.
2) Move the "aperture diaphragm lever" on the system
condenser to set the opening to 70 to 80% of the N.A.
(numerical aperture) of the objective. (-> Set the
"eyepiece tube turret" to position < B >, turn the Bertrand
lens focusing screw to perform focusing, and adjust the size
of the image while viewing the exit pupil of objective and
aperture diaphragm image.)
3) Move the "field diaphragm lever" on the dia-illuminator
until the size of the field diaphragm image is about the
same as that of the view field.
4) Move the "ND filter" on the dia-illuminator in and out of the
optical path to adjust the brightness of the view field.
Supplementary information
If precise color fidelity is not required, the brightness
can be adjusted by varying the lamp voltage using the
"brightness adjustment dial" on the left side of the
microscope.
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Field diaphragm image
Field of view
Field diaphragm image
Field of view