Nikon ECLIPSE TE2000-E Instructions Manual page 26

II. Microscopy
3. Center the annular diaphragm.
1) Set the "eyepiece tube turret" to position < B >, and turn
the Bertrand lens focusing screw to bring the annular
diaphragm image into focus.
2) Using a hexagon screwdriver, turn the two "annular
diaphragm centering screws" on the condenser module so
that the annular diaphragm image coincides with the phase
plate image in the objective.
3) Return the eyepiece tube turret to position < 0 >.
4. Conduct observation.
1) Fully open the "aperture diaphragm" of the condenser.
2) Move the "field diaphragm lever" on the dia-illuminator so
that the size of the field diaphragm image is about the same
as that of the view field.
3) Remove the "NCB11 filter" on the dia-illuminator from the
optical path, and move the "GIF filter" into the optical path.
(-> To improve the contrast)
4) Move the "ND filter" on the dia-illuminator in and out of the
optical path to adjust the brightness of the view field.
Supplementary information
If precise color fidelity is not required, the brightness
can be adjusted by varying the lamp voltage using the
"brightness adjustment dial" on the left side of the
microscope.
5. Conduct observations using an objective with a different magnification value.
1) Move a different Ph objective of desired magnification into
the optical path.
2) Turn the "condenser turret" and set it to the same Ph code
as that of the objective placed in the optical path in step 1).
3) Center the annular diaphragm placed in the optical path.
(-> See the procedure 3.)
Supplementary information
When the system condenser is installed with the Ph
condenser module, it is necessary to center all Ph
modules.
When the "ELWD-S condenser" is used, centering the
PhL diaphragm results in the centering of all annular
diaphragms.
6. Change the specimen.
Use the "objective refocusing ring" on the microscope main
body, the "tilting" of the dia-illuminator, and the
"condenser refocusing clamp" to facilitate specimen
replacement.
7. Upon the completion of microscopy
Follow the same procedure as for bright-field (BF)
microscopy.
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