Nikon ECLIPSE TE2000-E Instructions Manual page 50

III. Operation of each part
• When using the TE2000-E:
The fine adjustment function of the focusing module of the TE2000-E is motorized, and the fine focus knob is
provided on the right side of the main body. The distance of movement achieved by one rotation of the fine focus
knob can be set to Fine, Middle, or Coarse. (The knob is set to Coarse at the factory.) The current Z-axis position
can be also displayed. For details, refer to the instruction manual supplied with the T-HUBC HUB controller.
The coarse focus knob on the left side of the main body is not motorized. Note that the coarse focus knob turns
during motorized Escape/Refocus movements. The relationship between the amount of coarse focus knob
rotation and the distance of objective movement is the same as in the TE2000-U.
Supplementary information
The distance of movement achieved by one rotation of the fine focus knob is as follows. Note that these
distances are approximate and may vary depending on the knob-turning speed. The T-EFN focus knob
functions are identical.
Fine: 25 µm/rotation
Middle: 50 µm/rotation
Coarse: 100 µm/rotation
Also note that the distance per rotation of the coarse focus knob is 4.9 mm.
Coarse focus knob torque adjustment ring
Rotating the torque adjustment ring on the left side of the microscope counter-clockwise will make the rotation of the
coarse focus knob heavy.
Objective refocusing ring
The objective refocusing ring on the right side of the microscope is used to mark the position of the coarse focus knob
where a specimen is in focus.
Once the refocusing ring is clamped at the in-focus position, refocusing is much easier after shifting the objective for
specimen change or other purpose. All you have to do is rotate the coarse focus knob until it reaches the limit.
The refocusing ring is useful if, for example, the objective is very close to the stage because the container of the specimen
has a thick bottom plate, and the magnification can only be changed after lowering the objective.
When the refocusing ring is not used, be sure to turn it counterclockwise till the limit to loosen the clamp. If clamped, the
focusing mechanism cannot be moved up from the clamped position with the coarse focus knob. However, stage
movement with the fine focus knob is not affected.
1) At the position where a specimen is in focus, turn the refocusing ring clockwise to clamp the ring.
2) Lower the focusing mechanism by using only the coarse focus knob, and then change the objective.
3) Raise the focusing mechanism slowly to the limit, by using only the coarse focus knob. Here, the microscope is
roughly in focus. Then, rotate the fine focusing knob to give a sharp focus.
REFOCUS/ESCAPE switch
The REFOCUS/ESCAPE switches at the side of the focus knob on the right side of the microscope are used to mark the
vertical position of the objective when the specimen is in focus.
Pressing the ESCAPE switch (the lower switch) lowers the objective to the Escape position (2 mm below the standard
position) while memorizing the objective position at which the switch was pressed. Pressing the REFOCUS switch (the
upper switch) will raise and return the objective to the memorized position. Use these switches to facilitate refocusing
when the objective must be lowered for specimen changes and objective switching.
48
(not provided on TE2000-E)
(for TE2000-U only)
(for TE2000-E only)