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EG Konformitätserklärung EC Declaration of Conformity Name und Anschrift des Herstellers: Name and address of the manufacturer: Analytik Jena AG Konrad-Zuse-Straße 1 D-07745 Jena Hiermit erklären wir, dass das nachstehend beschriebene Produkt Herewith we declare, that the product described below Pipettierkopf ...
General Information General Information Note This User Manual informs about the setup and function of the Pipetting Head for the ® CyBio FeliX from Analytik Jena AG. This Manual further contains instructions and advice about general product care and the Manufacturer’s defined scope of maintenance work items. In addition, you may use this Manual to analyze fault situations for potential causes and take appropriate measures for fault removal. This User Manual must be readily available to operating and maintenance personnel at all times! NOTE The information contained herein reflects the latest state of knowledge at the moment of going to press. Analytik Jena AG reserves the right to make changes if deemed nec- essary in the interest of technical progress. Target Group This User Manual addresses: Personnel incorporating the Pipetting Head into a host system. Qualified and properly trained expert personnel who are able to operate the system and provide general care for the product ( “Requirements for Operating Personnel” on page 15). Employees with responsibility for: – the planning of process sequences/operating procedures, – preventive maintenance & cleaning work, – safety devices, etc. 2016...
General Information Conventions 1.3.1 Textual Markups Work instructions involving a timed sequence are numbered and merged to action units to specify related results. Enumeration involving no timed sequence is shown as a bullet-style list, sub-level numbering or as a dash-style list. Safety notes are marked by a pictogram and a signal word ( “Warning Signs” on page 3). Specific action-related safety notes will precede the actual instruction. For graphic design styles of cross-references, you are referred to the table below: Table 1: Graphic design styles of cross-references Type of cross-reference Graphic style Explanatory note Fig. 1 Reference to an illustration Illustration is on the same page Fig. 1 on page 11 Reference to an illustration ...
General Information 1.3.2 Warning Signs WARNING Indicates a potentially hazardous situation. May result in death or serious injury (crippling) if not avoided. CAUTION Dangerous situation! Potential consequences: light or moderate physical injury. NOTICE Dangerous situation! Potential consequences: material damage. NOTE Useful application advice, no potential danger involved. NOTE Note regarding environmental protection. 2016...
General Information Intended Purpose ® This pipetting head is intended for use in the CyBio FeliX (flexible pipetting platform for fully automated single-channel or multiple channel liquid handling). Its range of capabilities is restricted by the scope of integrated functional software and firmware, as well as the scope of delivery. For this reason, the user is prohibited from operating the product in any way other than specified in this User Manual. Conforming Use The Pipetting Head has been developed for fully automated processing of microplates and special sample holders in chemical and biological laboratories. In medical and diagnostic applications, conforming use is restricted to research. Its main functions are the aspiration and dispensing of liquid from and into microplates, columns (single wells or tubes). The conforming use is further defined as follows: the product is operated by qualified and trained research and laboratory personnel all operating requirements, procedural sequences and related safety notes quoted or described in this User Manual are duly observed all specifications in this Manual regarding product start-up, operation, preventive maintenance and care are met applicable safety standards or rules are always fulfilled. Any use other than or in excess of these rules will be regarded as non-conforming! The user will be solely liable for damage resulting from a case of non-conformance. NOTE In the event of non-conforming use of the pipettor, Analytik Jena AG will refuse war- ranty or liability claims for alleged material damage and personal injury! 2016...
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General Information The definition for non-conforming use includes: operating the product in medical laboratories of a non-research profile working with explosive substances working in an explosive atmosphere – operation in a potentially explosive zone is prohibited NOTE Operation involving dangerous substances will be at the sole responsibility of the user! This shall include compliance with all valid safety requirements for the protection of persons and material goods during work with radioactive, infectious, poisonous, corro- sive, combustible and other hazardous substances. The user is under obligation to fulfil all requirements for laboratory equipment and the conduct of personnel handling substances of this nature and on the practices in place for cleanliness, sterilization, environmental protection and waste disposal. The user is advised to issue special operating instructions where the product is to be operated with the involvement of hazardous substances. Accordingly, this User Manual contains no safety note warning of personal injury or material damage caused by substances being examined. Process control must rely on included Analytik Jena AG software. Alterations or damage to product software may give rise to faults in process flow and damage the product or its components. Software protection is the sole responsibility of the user. 2016...
General Information Warranty & Liability The period of warranty and scope of liabilities will be as stipulated under binding law and provided for in the General Terms of Business of Analytik Jena AG. Warranty will be limited to repair services or replacement of damaged parts. It will exclude consequential damage of any kind. Damage to wear & tear parts and cases of glass breakage are not covered by warranty. Any deviation from conforming use as defined in this User Manual (operating requirements, process sequences) will result in restricted acceptance of warranty or liability claims in the event of damage. In the event of personal injury or material damage, no claim for warranty and liability will be accepted, unless the product is operated as specified in section “Intended Purpose” on page 4. NOTE This loss-of-warranty clause shall apply to potential periods of interruption in business and to any product component that had not been directly affected by authorized warranty work. Scope of Delivery Included in delivery are: Pipetting Head (OL3318-XXX-14) Solid base Documentation 2016...
Technical Specifications Technical Specifications SELECT Head 8/250 μl General characteristics Designation SELECT Head 8/250 μl Type 8/250 μl Order number OL3318-14-100 Drawing number 30-3318-100-14 Materials Aluminium, stainless steel, polyethyl- ene (PE), silicone, polypropylene (PP) Specification Number of pipette tips Volume range 5 – 250 μl Volume range selectable in steps of 0.01 μl Precision in the volume range Variation coefficient 3 % > 10 – 25 μl Precision in the volume range Variation coefficient 2 % > 25 – 250 μl Pipette tip types ® CyBio Tips (accessories; „Accessories &...
Technical Specifications General characteristics Operation: Permissible ambient temperature +15 °C to +35 °C 75% at +35 °C Permissible relative air humidity no formation of condensate Storage & transportation: Permissible ambient temperature –10 °C to +50 °C 85% at +30 °C, Permissible relative air humidity no formation of condensate SELECT Head 8/50 μl General characteristics Designation SELECT Head 8/50 μl Type 8/50 μl Order number OL3318-14-200 Drawing number 30-3318-200-14 Materials Aluminium, stainless steel, polyethyl- ene (PE), silicone, polypropylene (PP) Specification Number of pipette tips Volume range 1.0 – 50 μl Volume range selectable in steps of 0.01 μl Precision in the volume range of Variation coefficient ...
Technical Specifications General characteristics Weight approximately 4.7 kg Storage & operating conditions Operation: Permissible ambient temperature +15 °C to +35 °C 75% at +35 °C Permissible relative air humidity no formation of condensate Storage & transportation: Permissible ambient temperature –10 °C to +50 °C 85% at +30 °C, Permissible relative air humidity no formation of condensate Offset Adapter General characteristics Designation Offset adapter Order number OL3317-11-290 Width 216.2 mm Depth 161.7 mm Height 12 mm Weight approx. 0.65 kg Motion extent 65 mm 2016...
Safety Notes Safety Notes General NOTE You should carefully read this chapter. Follow all safety notes that precede described action in the various chapters of this User Manual, as well as any message or advisory prompt that may be displayed on the monitor screen by control and evaluation software tools. In addition to the safety notes in this Manual and local safety practices as may be applicable to product operation from case to case, generally established accident prevention, industrial labor protection and environmental protection rules must be considered and duly observed. A reference to potential danger cannot be regarded as replacing the appropriate labor protection rule that must be observed in each particular case. Follow these general safety rules: Do not analyze or use aggressive substances of a type that may compromise the stable performance of the product or its components ( “Chemical Resistance” on page 17)! Do not make changes in product engineering design, unless by prior agreement with Analytik Jena AG! Do not manipulate or damage software or software configuration settings! Do not operate the product with safety devices in a defective state or with safety and protection devices installed in a nonconforming manner! Operate the product only at a line voltage that complies with nameplate specifications! Observe prescribed maintenance intervals! ( “Maintenance & Care” on page 51)! ...
Safety Notes Standards & Guidelines The Pipetting Head has been built to meet currently valid rules of technology and generally established requirements on safety engineering. The product and its components have been designed in accordance with basic safety and health requirements under applicable laws, standards and guideline regulations. All specifications relating to safety reference the European Union regulations in their latest binding revisions. Other specific national laws and regulations must equally be observed. 2016...
Safety Notes Safety Labeling NOTE Affixed warning notes and safety symbols are an integral part of the Pipetting Head and must be followed under any circumstances! Check warning labels and safety symbols for intactness and completeness before you begin any kind of start-up action. Do not proceed to start-up action if you have identified a missing or damaged warning note or safety symbol! Damaged or missing warning notes or safety symbols may lead to faulty action with consequential injury to persons or material damage to equipment! Warning notes and safety symbols must not be removed! Replace damaged warning labels and safety symbols immediately! The following safety symbols are affixed on the product: Table 2: Safety symbols Safety symbol Meaning Comment Warns of a Warns of mechanical haz- danger point ard from mechanically moving product parts Warns of hand injury Warns of crushing/pinch- ing by mechanically mov- ing parts 1 Also an integral part of a warning note. Warning note Meaning Comment Warns of crushing or Consider the pipetting pinching accidents head's weight for installa- tion or deinstallation work A general warning sign (“Warning of danger point“)
Safety Notes Mechanical Danger Zones – Notice NOTICE Mechanical motion of the Pipetting Head as an integral part of a host system may pose potential hazards to personnel. Always keep your hands/fingers clear of the mechanical motion range as the Pipetting Head (with pipette tips) performs vertical down-motion. There is danger of crushing/pinching one‘s hands! Mechanical Danger Zones Fig. Danger zone below the Pipetting Head In order to prevent dangerous situations, you are advised to follow these general rules: Consider its weight/mass when handling the Pipetting head. Do not place your hands or fingers into the head’s motion range as long as mechanical motion has not finished, nor should tools or aids be used to interfere ...
Safety Notes Requirements for Operating Personnel Requirements under specific product aspects: The product may not be started up, operated or maintained other than by duly trained expert personnel having received instructions on operational safety. Such training will also include familiarization with the contents of this Manual and manuals of related product components or additional equipment units as may be appropriate from case to case. The product must not be operated by minors or persons under the influence of alcohol, drugs or medication. A security schedule must be put in place to ensure that only authorized personnel can work with the product. Operating personnel must be aware of the potential dangers that emanate from substances being processed. Appropriate personal protective equipment should be applied if necessary. Requirements under specific laboratory aspects: Before a break in operation or on completion of work, adequate measures should be taken for skin cleaning and skin protection. You are prohibited from eating, drinking, smoking or using open a naked flame at or near the product installation site! Safety Requirements for Transportation Follow these general safety rules: ...
Safety Notes Safety Notes for Operation 3.8.1 General Operating personnel are obliged to convince themselves of the proper technical condition of the product and its components, including that of safety devices, before they can proceed to action for powering up. Notably, this requirement applies following a change in, an extension to or a repair of the product. Do not operate the product, unless all protective devices are in place, properly installed and fully functional. Make sure that all ventilation devices of the Pipetting Head are in a fully functional condition. Obstructed ventilation grids, vents, etc. may cause operating trouble or damage to the product. Do not introduce objects/aids into a product opening and prevent penetration of liquid into the inner space of the Pipetting Head through openings or joints. Do not use aggressive substances of a type that may compromise the stable performance of the Pipetting Head ( “Chemical Resistance” on page 17). Use only accessory items, consumable materials and spare parts provided or recommended by Analytik Jena AG! 3.8.2 Explosion Proofness, Fire Prevention The product must not be operated in an explosive environment or using explosive substances. ...
Safety Notes 3.8.4 Fundamental Maintenance & Care Rules Maintenance may not be carried out by anyone other than service personnel of Analytik Jena AG or expert personnel properly trained and duly authorized by Analytik Jena AG. Unauthorized maintenance work may cause damage. Always turn product power off before you perform work for maintenance or cleaning of the product. Pull the main power plug from the power socket at first. Use only original accessory items and original spare parts or such accessories and spare parts that are recommended by Analytik Jena AG. 3.8.5 Handling of Dangerous Substances The product owner will be solely responsible for compliance with all safety requirements in place for the protection of persons and material goods during work involving radioactive, infectious, toxic, etching, combustible and other dangerous substances. The product owner/operator is advised to establish a special operating practice for product operation with involvement of dangerous substances. This User Manual includes no safety notes aimed at preventing personal injury or material damage that may be caused by substances being examined. 3.8.6 Chemical Resistance Analytik Jena AG will refuse any responsibility for examination of aggressive substances that may deteriorate the stability of the product or its components. Particular caution should be used when handling leaches, acids or organic solvents, because these may reduce the product's lifetime. ...
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Safety Notes Components in direct physical contact with substances being processed: Table 3: Components Component Material Pipette tips Piston seals PE-HD 1 Aerosols are likely to establish indirect contact between substances and piston seals. The pistons are made of stainless steel, the piston seals of polyethylene (high density). None of the components mentioned above will be resistent when exposed to any of the following substances: Table 4: Substances Substances Hydrofluoric acid (HF) Highly concentrated acids Cleaning powder Paint thinners Naphtha (raw gasoline) Gasoline Acetone Cleaning spray Ozone Solutions including oxidative substances 1 This table is not exhaustive Do not use solvents (thinners), aggressive detergents, flammable liquids or etching leaches for cleaning. Substances of this kind may cause damage to shielding parts. NOTE Consult with Analytik Jena AG in case of doubt. 2016...
Safety Notes Rules of Conduct in Cases of Emergency Use the product’s main power switch (On/Off switch) immediately on noticing a dangerous situation and/or disconnect the power plug from the line socket in this case! Since prompt reaction can save lives in a situation of danger, make sure that the following requirements are met: – Operating personnel must be aware of the locations and the proper handling of safety devices, accident and hazard alarms, as well as first-aid kits and emergency/rescue equipment. – The product owner/operator will be responsible for providing adequate training for operating personnel. – All first-aid items (medical chest, eyewash bottles, stretchers, etc.) and fire fighting equipment (fire extinguishers) must be kept within easy reach and readily available at all times. Related equipment must be in a fault-free condition and undergo regular inspection for normal operating condition. Actuation of Emergency Shutdown You should familiarize yourself thoroughly with all documentation parts relating to the host system that integrates your Pipetting Head. 2016...
Technical Description Technical Description Setup 4.1.1 Pipetting Head Fig. Pipetting Head (principal setup) Pipetting Head – upper part with electrical connection “Electrical Connection” on page 23 Middle part (pipetting base) “Head Mount Dovetail Guide” on page 23 “Nameplate” on page 22 “Safety Labeling” on page 13 Pipetting Head – lower part – Tip holder – Light (LED) 2016...
Technical Description 4.1.2 Nameplate Fig. Nameplate at Pipetting Head Nameplate specifications/details: Manufacturer’s data Product information (type designation, tradename) Identification code (model & serial number) Year of manufacture Components 4.2.1 Pipetting Head The pipetting head provide support bases (cone(s)) for mounting the pipette tips onto. Fig. Pipetting Head (bottom view) Cone O-ring For further information about accessories, you should consult chapter 11 ® (CyBio FeliX Basic Unit). 2016...
Technical Description 4.2.2 Head Mount Dovetail Guide Fig. Dovetail guide at the Pipetting Head 4.2.3 Electrical Connection Fig. Electrical connection (connector terminal) 2016...
Technical Description 4.2.4 Offset Adapter Fig. Offset adapter The offset adapter (OL3317-11-290 Fig. 7) is required to allow unrestricted Hit-Pick- ing with the SELECT Head. Two special positions (13th and 14th) are provided. Technical specification “Offset Adapter” on page 9. 2016...
Technical Description Mode of Operation The pipetting head works based on the principle of air displacement. The pipette tips and their related internal seals create air spaces. Pistons perform motion within these air spaces. They are powered by a separate drive (engine). Liquid is drawn in via channels (1 – 8). Piston motion occurs in steps of 0.1 μm, thus achieving an accuracy/resolution that cor- responds to a fraction of a microliter. Further main factors of influence on accuracy/resolution performance are the: – wettability of tips – dimensional accuracy of outlet openings – way the system and liquid are handled. Further information “Precision” on page 36. NOTE Piston motion creates negative pressure or positive pressure, thus causing liquid to be as- pirated or released. Upon reaching pressure equalization state, the current process is completed. The time required to reach this state depends - among other factors - on the properties of the liquid being handled. ® For further information, please refer to the CyBio FeliX Manual. 4.3.1 Pipetting The term pipetting means that a defined volume of liquid is aspirated (with/without additional stroke) from a source to be subsequently dispensed into a destination or a waste bin (with/without blow-out function). For pipetting, we distinguish between aspiration without additional stroke (simple transfer) and aspiration with additional stroke ( “Aspiration Without Additional Stroke” on page 29 and “Aspiration With Additional Stroke”...
Technical Description 4.3.2 Dispensing The term dispensing means that a given aspired volume of liquid is discharged again in several sub-volume portions. For dispensing, a total volume must be taken in with additional stroke. Blow-out is performed in a separate position – in some cases, extra volume may have to be aspired, for example, where viscous liquids are handled or a great number of partial liquid discharges are required. A particular feature distinguishing all air displacement systems is that a thermodynamic equilibrium must be established in the air volume before dispensing may begin. For this reason, the initial portion – after a short pause – should be discharged back into the source, before (precise) dispensing into the target plate may start. 4.3.3 Tip Rinsing The behaviour of liquid on the surface of a solid body is different for surfaces already wetted and surfaces unwetted. Greater precision is achieved where a tip had been wetted prior to dispensing or pipetting. The behavior of liquid will remain reproducible for each process in this case. The recommended procedure is a triple rinsing cycle with the maximum possible volume of an installed tip. 4.3.4 Residual Discharge (Blow-Out) Having dispensed its “last“ volume, the piston performs downward-motion into lower- most end position, in order to ensure that no liquid will remain in the tips. This process is referred to as blow-out. NOTE If blow-out is performed into a liquid, bubbles may form. 4.3.5 Piston Zero-Position On completion of blow-out action, the piston returns into zero-position, thus restoring its starting position for aspiration of liquid. NOTICE Piston motion into zero-position must not occur in liquid. Otherwise, there is danger of piston contamination or destruction of the pipetting head. Operating Modes Different operating modes are available. ®...
Technical Description Volume Cycle Liquid volumes are aspirated and discharged by piston motion. The mechanical design of piston suspension implies a certain freeplay (backlash). This means that after each reversal of the direction of piston motion, the piston drive travels a certain path length without producing actual piston motion, i.e. without aspirating or ejecting liquid. This path length corresponds to the amount of freeplay. In order to prevent pipetting errors, a volume cycle is organized in a manner to ensure that the piston reversal point is located outside of the actual pipetting range (motion to zero-position, aspiration with additional stroke) or the reversal zone will be compensated by appropriate motion, respectively (discharge with blow-out) 4.5.1 Aspiration With Additional Stroke Fig. Aspiration with additional stroke Zero-position A1 = D2: Nominal pipetting volume Aspirate volume with Additional aspiration volume additional stroke and reverse Discharged additional volume < A2 piston direction (additional stroke) Upper freeplay Nominal pipetting volume Exactly dispense volume (single Residual volume (proportional to volume or partial volumes) freeplay) Residual discharge Motion to zero-position Starting from zero-position (1), the pistons move beyond the position that corresponds to the nominal pipetting volume. Subsequently, they are lowered back into this ...
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Technical Description The upper freeplay zone (3) is thus overcome. In the course of direction reversal, additional volume (D1) is discharged. As a consequence of piston reversal, this additional amount of volume is smaller than the volume of additional stroke motion (A2). What actually remains in the tip is the nominal pipetting volume (D2) and a residual volume (D3). In the next step (4) exact volume portions can be discharged as follows: in a single step ( “Pipetting” on page 25) or in several steps ( “Dispensing” on page 26). Once the nominal pipetting volume has been fully ejected, the residual volume (D3), which results from the piston’s upper direction reversal, will be contained in the pipette tip. This remainder is now discharged into a waste box or back into the source with a blow-out (5) function ( “Residual Discharge (Blow-Out)” on page 26). A volume cycle is completed on "Motion to zero-position". The "Move pistons into zero-position" (6) command triggers upward piston motion into the starting position, overcoming the lower amount of freeplay. This process step must be performed without liquid (tips cannot make contact with liquid). If liquid is aspirated in the course of this step, this is going to cause dosing errors and may, in some cases, even result in contamination of pistons or destruction of the pipetting head. 2016...
Technical Description 4.5.2 Aspiration Without Additional Stroke Fig. Aspiration without additional stroke Zero-position A1=D1: Nominal pipetting volume Aspiration Upper freeplay Discharge with additional stroke Motion to zero-position Where liquid is taken up without additional stroke motion, it must be discharged in a single step in order to achieve precise results. As a vital by-condition, the motion length for discharging (4) must be greater than the motion length for aspiration (2). Because the point of piston motion reversal is located within the motion area for discharging, no volume is released as the upper freeplay zone is overcome (3). For discharging, a greater motion length is travelled than that required for the nominal volume; the whole volume is discharged, followed by the ejection of air (D2). Having received a "Move pistons into zero-position“ (5) command, the pistons will move up into their starting position, overcoming the lower amount of freeplay as they do This process step must be performed without the involvement of liquid (tips cannot make contact with liquid). If liquid is aspirated in the course of this step, this is going to cause dosing errors and may, in some cases, even result in contamination of pistons or destruction of the pipetting head. 2016...
Packaging, Transportation & Storage Packaging, Transportation & Storage Pipetting Head Abb. 10: Pipetting head with solid base Pipetting head Solid base 2016...
Packaging, Transportation & Storage Safety Notes NOTICE Environmental influences, mechanical shocks or formation of condensed water may de- stroy individual product components! Adequate precautions should be taken to protect all components from environmental impacts, mechanical shock or formation of condensed water during transportation or shipment! Temporary open-air storage of the product is forbidden! NOTICE There is potential damage from improper packaging! The Pipetting Head, including its accessory parts, may only be shipped or transported in original packing! Description 5.3.1 Packaging To prepare the Pipetting Head for shipment or transportation, proceed as follows: 1. Drain residual liquid, if any, from pipette tips. 2. Loosen and remove the tips. CAUTION Danger of crushing/pinching! There is danger of crushing one’s fingers. 3. Remove Pipetting Head from the pipettor. 4. Place Pipetting Head into original packing! 5.
Routine Start-Up Procedure Routine Start-Up Procedure Safety Notes The requirements regarding operating climate are as follows: Temperature range: +15 °C to 35 °C Permissible relative air humidity: 75 % at 35 °C, no formation of condensate The atmosphere inside the operating room should be dust-free to a maximum possible degree, free from drafts and free from etching vapours. You are prohibited from smoking in the operating room. The following rules should be observed: The operating room must have a stable, horizontal, dry and vibration-free floor. Do not install the system directly at doors or windows nor close to sources of electromagnetic interference. Prevent direct exposure to sunlight and radiation emitted by heaters. Provide air conditioning for the room if necessary. Provide easy access to all system parts at all times and do not obstruct ventilation slots by placing other equipment or fixtures on such inlet/outlet positions. Allow enough time for the system to adjust to the installation site temperature, especially where storage and installation are in different locations. Protective caps Fig. 11: Fig. 11: Protective caps Start-up procedure: Remove protective caps. 2016...
Routine Start-Up Procedure 6.1.1 Energy Supplies ® Applies in conjunction with the instrument CyBio FeliX: WARNING In the event of a break in protective conductor wiring, there is life-threaten ding danger due to electrical shock! Insert the main power plug only into a mains socket with a grounded PE contact! Make sure that the protection effect is not rendered ineffective by extension cables without a PE conductor or by the use of a voltage regulating transformer! CAUTION Operation at a mains voltage level or frequency other than specified on the nameplate may result in destruction of the pipettor! Make sure that power supply specifications in the operating room do agree with those on the nameplate! You are prohibited from starting the system up if there is a mismatch in specifications. The system requires a single-phase alternating current net for normal operation. The system includes a wide-range power pack. It is rated for AC voltage levels of 115/230 V and a frequency of 50/60 Hz. You should make absolutely certain that nameplate spec- ifications are actually met and power is supplied with values as indicated on the name- plate. Initial Start-Up & Configuration Because of the system's complexity and to guarantee its failsafe function, work for installation, initial start-up and configuration on your premises may be performed by noone other than Analytik Jena AG service personnel or duly authorized expert techni- cians. Initial start-up essentially includes: Installation and adjustment of system components Providing cable connections and connecting power supply cables Software installation (factory-performed) and configuration Briefing and on-the-job training Check shipment for integrity, completeness and agreement when unpacking the vari- ous system units.
Routine Start-Up Procedure Function Tests NOTE On completion of manufacturing, the test procedures described in this chapter are car- ried out in standardized conditions (test & metrology room). Resulting test reports are included in the scope of delivery. Further information: Applikation Note „Photometric and Gravimetric Liquid Handling Check Procedure to determine the Random Error (Precision) and Systematic Error (Accu- racy) of Automatic Liquid Handling Systems (ALHS). 6.3.1 Leak Leak testing is necessary to ensure that the pipetting head does not leak liquid. Subject to testing are the pistons, the pipette tips and the O-rings. A leak test is performed drawing a particular volume of dye solution into the pipette tip(s) and watching their liquid level over a time of 30 minutes. Procedure: Make sure that the pipettor is in a horizontal position. To do this, carefully place a water level on its transporting unit. Use setting screws as necessary to adjust horizontal position. Remove tips. Inspect O-rings thoroughly for cracks or sedimentation with the help of a suitable light source. (Replace on detection of obvious signs of wear and tear. Tips take on. Place reagent reservoir in position below the tips. Bring reagent reservoir close to the tips — the pipette tips should dip below the surface of dye solution by not less than 2 mm. Wet the pipette tips on their inside by performing five rinse cycles. Aspirate 50 % (of maximum tip volume) of dye solution. NOTE For leak testing, the pipette tips must penetrate into the liquid to a depth not less than 2 mm. This is necessary to exclude the possibility of droplets forming at their end, because these would falsify the result of testing.
Routine Start-Up Procedure 6.3.2 Precision Testing for variation coefficient CV (percentage standard deviation) is performed using a 96-well microplate with transparent flat bottom and dye solution. A suitable vertical photometer serves as the measuring tool. Its own precision must have been tested/verified and documented prior to measurement. Depending on the head type, different test volumes are provided. Material/preparation: 96-well microplate with flat transparent bottom. 0,1 N NaOH solution Dye (e.g. p-nitrophenol) Film for taping plates off Microplate shaker Microplate centrifuge Table 5: Aspiration and test volume Head type Prefill Test p-nitrophenol volume volume dye solution* 8/50 μl 197 μl MP 96 3 μl 8 mM ...
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Routine Start-Up Procedure Rinse new tips prior to precision measurement using appropriate p-nitrophenol solution and test settings as follows: Piston speed: 1/3 of the default speed Number of rinsing cycles: Rinsing volume: maximal volume (tip) 1. Use only new plates and new tips. 2. Total volume is 200 μl for 96-well plates. 3. Pause after every aspiration/dispense stroke; 1 second duration. 4. Rinse the tips with the above specified parameters (before the actual precision measurement). 5. Aspiration stroke always with an additional volume (SELECT Head 8/50 μl, suc- tion stroke 10 μl). 6. Return the first pipetting cycle back to the source. 7. Insert the tip about 1 mm in the flask (NaOH). 8. Pipette test volume as a portion of the total volume into test plate with NaOH prefilling. 9. Eject residual volume via blow-out back into source or waste box. 10. Seal (tape) off immediately. 11. Shake plate (on orbital shaker, parameter: wait for 30 minutes, at least). 12. Subject plate to hydro-extraction for removal of bubbles; parameter: for 2 minutes at 2000 r.p.m. 13. Do not measure results earlier than 60 minutes thereafter. Table 6: : Mixing times 96-well microplate Orbital shaker of...
Routine Start-Up Procedure 6.3.3 Accuracy Accuracy defines the degree of agreement between a measured dispensed volume (mean value of all measurements in a 96-well microplate) and a specified (target) vol- ume. Material / preparation: Laboratory scales with resolution not less than 1 mg The lab scale must be calibrated regularly (check the calibration mark). Lidded 96-well microplate with flat bottom. NOTICE Since new microplates are, typically, vacuum-packaged (sealed), the selected test plates must be unpacked not later than one week before testing (the weight of a new microplate may decrease due to evaporation or increase due to absorption). Accuracy testing is performed with de-ionized water (1 bar and 998 mg/cm³). Test procedure: Place a reservoir with de-ionized water in a position of the transporting unit. For preliminary wetting of tips, use rinsing procedure as follows: number of cycles 5, with test volume. Place microplates in a different position. Prefill a volume of 50 μl. Weigh microplate in prefilled lidded condition. Unlid plate and deposit it in a place. Dispense desired volume into the microplate immediately thereafter (Method “Precision” on page 36). ...
Routine Start-Up Procedure Software 6.4.1 General ® The standard configuration of the CyBio Composer assumes operation without the SELECT Head. Therefore, at first, the respective instructions must be visualized page 40. Besides, unrestricted hit-picking with the SELECT Head can only be performed at two specific positions (location 13 and location 14), which are created by placing two offset adapters of type OL3317-11-290 ( Fig. 7 on page 24) on the lower deck. The position 13 is located between the device positions 1 and 4, the position 14 between the device positions 2 and 5. ® To use the new positions in the CyBio Composer they must be added to the configu- ration and their properties defined page 41. 2016...
Routine Start-Up Procedure 6.4.2 Start-up Start by carrying out the following actions to vosualize the hit-picking instructions – „Change selection“ and – „Change offset“ : 1. Check to make sure that the Composer version 2.63 is installed. 2. Set the hit-picking entry in the instruction availability “Yes” Fig. 12. Fig. 12: Hit-picking After visualizing in the configuration, the instruction toolbar of the composer must be reset. For that: 3. Right-click on the menu item “Edit instructions“ in the “Instructions“ window 4. Right-click on the menu item “Reset all entries“ in the “Instructions“ window 5. Right-click on the menu item “Edit instructions“ in the “Instructions“ window 1 Or higher. 2016...
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Routine Start-Up Procedure After resetting the instructions toolbar the hit-picking instructions “Change selection“ and “Change offset” are displayed Fig. 13. Fig. 13: Hit-picking Commands ® 6. Add location 13 and location 14 by calling the entry “Locations for CyBio FeliX - Virtual 3D drive“ Fig. 14 in the “Adapter Management Service“, and create the location 13 and the location 14 in the configuration (add “No adapter“). Fig. 14: Adapter management service 2016...
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Routine Start-Up Procedure 7. Create two new relative positions – as shown Fig. 15. Fig. 15: Positions 13 and 14 8. Make the following settings Fig. 16. Fig. 16: Settings for positions 13 and 14 2016...
Operation Operation Safety Notes CAUTION Dangerous situation! Beware of crushing of pinching effects – the Pipetting Head weighs approximately 5 kg. Always hold the head with both hands. CAUTION Danger of crushing! Do not place your hands or fingers into the motion range for tips or Pipetting Head mo- tion. NOTICE Dangerous situation! Motion into zero-position must always be performed without any liquid, in order to prevent damage! Operation You should thoroughly familiarize yourself wit all information and explanatory notes ® contained in chapter 7 of the CyBio FeliX Manual. 2016...
Operation Software controlled operation ® The SELECT Head for the CyBio FeliX is controlled by the software ® (CyBio Composer ). More information about the instructions are available in the Windows Help function (PluginFeliX). 7.3.1 Instructions (overview) Symbol Function Meaning – The instruction activates one or several pipette Change selection tips (Auswahl ändern) – More information ““Change selection” instruction” on page 45 – Set the current offset between the activated Change offset and the non-activated pipette tips of the (Versatz ändern) SELECT Head in units of millimeter –...
Operation 7.3.2 “Change selection” instruction Identification of the pipette tips: The hindmost pipette tip (seen from the front of the device) carries the identification mark A1, the frontmost pipette tip the identification mark H1. Example: Selection “C1-F1;H1“ means that the four middle pipette tips and the front most pipette tip are activated. (Thus, the pipette tips are selected by the same procedure as the wells on a microtiter plate are selected, and the format is also the same.) The instruction “Change selection“ affects all following pipetting and movement in- structions as follows: Instruction / command / Meaning variable On a microtiter plate, always the hindmost „Labware anfahren“ „Move to labware“ activated pipette tip moves to the position specified in the instruction. The volume to be aspirated or dispended must al- „Aspirieren/Dispensieren“ ways specify „Aspirate/Dispense“ either individual volumes relating to all acti- vated pipette tips at a time. Example: “22.3“ ...
Operation 7.3.3 “Change offset“ instruction NOTICE Make sure that the change of the offset does not lead to a crash with the labware used or with the device. Observe the following: The maximum offset is limited to - 50.0 mm. The offset change is only applied until the next movement instruction is executed and is also disabled by the execution of the instruction “Change selection“. The offset between activated and not activated pipette tips can only be changed when the pipette tips are also loaded at the SELECT Head and at least one of these pipette tips is activated. Please observe the following variables overview: Variable Meaning „Versatz des aktivierten Spit- Displays the current offset. zenbereiches“ „Activated tip range offset“ Request to know whether any pipette tips are loaded „Angebrachter Spitzentyp“ at the SELECT Head (blank if no pipette tips are „Loaded tip type“ loaded or not known). Request to know whether any pipette tips are loaded ...
“What to do if...“ “What to do if...“ Safety Notes NOTE Refrain from unauthorized repair work, notably, repair work on the Pipetting Head. This may render potential claims for warranty null and void. WARNING Danger as a result of electric voltage! Observe all safety rules when you inspect cabling for possible faults. Description Check all potential fault sources on occurrence of a fault. If problems are found to persist after this check or on identification of an undescribed fault, you should notify the Customer Service of Analytik Jena AG. You should also follow any of these advisory notes when you perform fault location or fault assessment: Always check for any noise other than normal operating noise, Make sure that connected assemblies are firmly seated, Track any increasing freeplay in mechanically moving assemblies (effects of wear & tear). Further advice on trouble shooting or fault removal is contained in chapter 8 of the ® CyBio FeliX User Manual. 2016...
“What to do if...“ Fault patterns/fault messages Fault pattern/fault message Check to see whether ... Instruction “Change selection“ the instructions are visualized in the device con- or “Change offset“ not available. figuration; reset the instructions on the instruc- tion toolbar „Initial Start-Up & Configuration” on page 34. There is no pipette response to the required pipette heads at the SELECT the instructions “Aspirate / Dis- Head are activated. pense“, “Pistons to zero posi- tion“ or “Rinse / mix“. Instructions “Change selection“...
Maintenance & Care Maintenance & Care Safety Notes WARNING Please note that physical contact with voltage-carrying product parts may lead to body injury or even death! Turn system power off and detach power cable from the line power socket before you proceed to any kind of work for maintenance or care! Take adequate precautions to protect the product from accidental restoration of power! Operating personnel are prohibited from performing work for maintenance, repair or adjustment of voltage-carrying system parts! Maintenance, repair or adjustment of product modules under electrical voltage may only be carried out by a qualified electrician! CAUTION Penetrating liquid may cause material damage to electrical and electronic components! Make sure that no liquid can penetrate into the inner space during any kind of maintenance or care. NOTE Intervention to mechanical or electronic parts in the inner Pipetting Head space may not be performed by anyone other than customer service personnel or specially authorized expert technicians. To ensure that your Pipetting Head will keep a state of optimal adjustment and faultless function over a longer period of time, we recommend the conclusion of a service/maintenance contract with Analytik Jena AG. 2016...
Maintenance & Care Maintenance Work Perform care and maintenance work tasks at regular intervals as specified herein and following these general advisory rules: Contamination and natural wear of modules give rise to increased strain levels and, hence, an increased probability of failure. Check for signs of wear and tear on assemblies under mechanical strain and initiate necessary replacements promptly on identifying a case of wear and tear. All systems parts capable of manual or motorized motion are subject to natural wear. Similarly, electronic components have no unlimited lifetime. NOTE Dirt, e. g. dried-on liquid, may increase wear dramatically in some cases. Always maintain clean working conditions! 9.2.1 Overview Table 7: Maintenance summary table Maintenance action Maintenance intervals As Weekly Monthly required “Wipe Down Casing” on page 53 2016...
Maintenance & Care Maintenance/Inspection Instructions 9.3.1 Wipe Down Casing NOTICE Never use cleaning powder, paint thinner or solvents like gasoline, acetone or Isopropanol to clean the Pipetting Head. Substances of this type will attack the surface of enclosure panels (for more details refer to: “Chemical Resistance” on page 17). Use a soft piece of cloth slightly moistened with mild soap or disinfectant solution to clean system enclosure parts. Commercially available disinfectant wipes may equally be used for cleaning. Spraying disinfectants or similar agents onto a system surface is classified as dangerous action and is therefore prohibited. Spray contains gases that can ignite. Do not apply force for cleaning. 2016...
Maintenance & Care 9.3.2 Replacement of O-Rings Operation involves O-rings (where a liquid handling adapter is required). They are sub- ject to permanent mechanical stress through contact with the pipette tips. Accordingly, for reasons of safety and to ensure the absence of leakage, the cone O- rings must be replaced at regular intervals. Where a maintenance contract has been concluded, replacement of O-rings will be performed by Analytik Jena AG service per- sonnel as part of each on-site maintenance package. Generally, O-rings should be re- placed in the case of obvious signs of wear and tear. This can be done with tooling included in delivery. Preparation: 1. Turn system power off. 2. Take O-rings and push them one by one onto the O-ring guide rod. 3. Take O-ring guide rod with mounted O-rings, insert the rod into its designated opening and push it in until mechanical stop position. 2016...
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Maintenance & Care Removal of old O-ring: 1. Hold the O-ring mounting tool with its designated cone end facing forward, then push the tool with the groove at O-ring height in the direction of the cone. Then exert moderate pressure and finally remove the O-ring mounting tool and the O-ring from the cone. 2. If your attempt at O-ring removal fails, there is still an alternative method for removal of the O-ring. Turn the O-ring mounting tool around for this purpose. There is a tip provided on the side of the O- ring guide rod that can equally be used to remove the O-ring. To do this, press the tip against the O- ring and remove the ring. 2016...
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Maintenance & Care Mounting the new O-ring 1. Turn the O-ring mounting tool until the O-ring guide rod faces toward the top, then slightly press against the cone. 2. Slowly push one of the string- threaded O-rings along the O-ring guide rod towards the cone until reaching the groove. Work can now be resumed in normal operating mode. 2016...
Shutting Down Shutting Down CAUTION There is danger of physical injury and damage to the product if cabling is removed in power-on state! Do not remove cables as long as they are energized! Make absolutely certain that power has been turn off before you remove a cable! Make sure that all of the following requirements are met: Tips have been removed. Line-power switch is in position "0". Power cable is unplugged from power multiple or line power socket. All microplates, reservoirs have been removed. The product and its components have been properly cleaned and disinfected in accordance with the rules for handling of currently processed materials and substances. The product is protected from sedimentation of dust. 2016...
Accessories & Spare Parts Accessories & Spare Parts NOTE The pipettor and its accessories are factory-matched to each other. Use only accessories recommended by the manufacturer. The manufacturer will refuse any warranty in the event of damage or function failure as a result of system operation with unspecified accessory items. 11.1 Accessories Additional information: see Price list. 11.1.1 Pipette Tips Table 8: Pipette tips Pipetting head Tip type Ordering code ® SELECT Head CyBio TipBox 96/50 μl OL3811-25-535-N 8/50 μl PCR-certified ® SELECT Head CyBio TipBox 96/250 μl OL3811-25-637-S 8/250 μl PCR-certified, pre-sterilized 11.2...
Waste Disposal Waste Disposal 12.1 Consumables NOTE Consumable materials must be disposed in accordance with binding workplace safety and environmental provisions of law. 12.2 System, Components & Accessories NOTE Unless otherwise agreed, the pipettor system, including any of its components, must be disposed in accordance with currently binding regulations of law on termi- nation of use. Action for disposal will be the responsibility of the system owner. NOTE The procedures to be followed for disposal, including parts thereof, are legally based on these EC Directives and related rules for implementation in national legislations within the EC: EC Directive 2002/95/EC (RoHS) EC Directive 2002/96/EC (waste electrical and electronic equipment) 2016...