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Instrument Testing
Define the Absorbance Test Plate Parameters
Obtain the certificates that came with the Test Plate.
1.
Start Gen5. From the main screen, click
2.
Add/Modify
Click
Add/Add
3.
Select the appropriate plate type and enter the plate's serial number.
4.
Enter the last and next certification dates, found on the calibration sticker on the
5.
Test Plate. Click
the
OD
and peak wavelength values.
Review the values you entered, and then click
6.
wavelengths and corresponding calibration data that have been entered will now
be available in Gen5 each time the Absorbance Plate Test is performed.
Procedure
From the main screen, click
1.
If prompted, select the desired Test Plate and click OK.
2.
When the
3.
Wavelength
Highlight the wavelength or wavelengths to be included in this test. Select only
4.
those wavelengths that are most appropriate for your use of the reader. Enter
any comments, if desired.
Start
Click
5.
Place the Test Plate in the microplate carrier so that well
6.
corner of the carrier (as you are facing the carrier).
Click
OK
to run the test.
7.
When the test is completed, the results report appears. Scroll through the
8.
report; every result should show PASS. If any result shows "FAIL", try the
following and rerun the test. If the test continues to fail, contact Technical
Support.
Make sure the information entered into Gen5 matches the Test Plate's
Certificate.
Verify that the Test Plate is within its calibration certification period. If it is
out of date, contact Technical Support to schedule a recertification.
Ensure that the Test Plate is correctly seated in the microplate carrier.
Check the alignment (corner) holes on the Test Plate to ensure they are clear
of debris.
Check the filters on the Test Plate to ensure they are clean. If necessary,
clean them with lens paper. Do not remove the filters from the test plate. Do
not use alcohol or other cleaning agents.
Agilent BioTek Epoch Instructions for Use
7201009I Revision C
Plates.
Plate. The
Absorbance Test Plate
Help
for guidance when setting the wavelengths and entering
System > Diagnostics > Test Plates > Run.
Absorbance Test Plate Options
Test, if it is not already selected.
Test.
System > Diagnostics > Test Plates >
dialog appears.
OK
to save the data. The
dialog appears, select
A1
is in the left-rear
Agilent Technologies, Inc.
Page 24 of 31
Perform Peak
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