Optical Performance - Agilent Technologies BioTek Epoch Instructions For Use Manual

Absorbance microplate spectrophotometer
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Specifications

Optical Performance

All qualifications were conducted using 96-/384-well, flat-bottom microplates.
For the performance described here, the
Measurement Range: 0.000 to 4.000 OD
Accuracy, NIST Filters
96-well plate,
normal read
0.000–2.000 OD +/-1% +/-0.010 OD
2.000–2.500 OD +/-3% +/-0.010 OD
384-well plate, normal read speed, 100-ms delay after plate movement
0.000–1.500 OD +/-2% +/-0.010 OD
1.500–2.000 OD +/-5% +/-0.010 OD
96-well and 384-well plate,
0.000–1.000 OD +/-1% +/-0.010 OD
Linearity
96-well plate, normal read speed, 100-ms delay after plate movement
0.000–2.000 OD +/-1% +/-0.010 OD
2.000–2.500 OD +/-3% +/-0.010 OD
384-well plate, normal read speed, 100-ms delay after plate movement
0.000–1.500 OD +/-2% +/-0.010 OD
1.500–2.000 OD +/-5% +/-0.010 OD
96-well and 384-well plate, sweep read speed
0.000–1.000 OD +/-1% +/-0.010 OD
Repeatability, NIST Filters, Standard Deviation
96-well plate, normal read speed, 100-ms delay after plate movement
0.000–2.000 OD +/-1% +/-0.005 OD
2.000–2.500 OD +/-3% +/-0.005 OD
384-well plate, normal read speed, 100-ms delay after plate movement
0.000–1.500 OD +/-1% +/-0.005 OD
1.500–2.000 OD +/-3% +/-0.005 OD
96-well and 384-well plate, sweep read speed
0.000–1.000 OD +/-2% +/-0.010 OD
Agilent BioTek Epoch Instructions for Use
7201009I Revision C
Accuracy, Linearity, Repeatability
Gain
speed, 100-ms
delay after plate movement
sweep
read speed
on the
Optics Test
should be <= 8.
Resolution: 0.0001 OD
Agilent Technologies, Inc.
Page 27 of 31

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