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Agilent BioTek Epoch 2
Absorbance Microplate
Spectrophotometer
ERRATA NOTICE: This document
contains references to BioTek.
Please note that BioTek is now
Agilent. For more information, go to
www.agilent.com/lifesciences/biotek.
INSTRUCTIONS FOR USE
Document part number 1771011I
Agilent Technologies, Inc.
For In Vitro Diagnostic Use
Revision C
May 2022
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Summary of Contents for Agilent Technologies BioTek Epoch 2
- Page 1 Agilent BioTek Epoch 2 Absorbance Microplate Spectrophotometer INSTRUCTIONS FOR USE ERRATA NOTICE: This document Document part number 1771011I contains references to BioTek. Revision C Please note that BioTek is now Agilent Technologies, Inc. Agilent. For more information, go to May 2022 www.agilent.com/lifesciences/biotek.
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Page 2: Table Of Contents
Install the Cuvette Holder ....................19 Turn on the Reader and Run a System Test ..............19 Establish Communication ....................20 Troubleshooting ..................... 20 Verify Performance ......................20 Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... - Page 3 Procedure ....................... 41 Clean Exposed Surfaces ....................41 Decontamination ......................42 Required Materials ....................42 Procedure ....................... 42 Instrument Testing ......................44 Schedule ......................... 44 System Test ........................44 Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C...
- Page 4 Test Plate Certificates .................... 45 Define the Absorbance Test Plate Parameters ............45 Procedure ....................... 45 Specifications ........................47 General Specifications ....................47 Read Specifications......................48 Optical Performance ...................... 49 Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C...
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Page 5: Preface
Preface Copyright © Agilent Technologies, Inc. 2021, 2022. No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. -
Page 6: Responsible Person (Ukrp)
Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 7: Warnings And Precautions
-CAUTION- recommended diluted sodium hypochlorite solution for more than 20 minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 8: Components
Users must evaluate this instrument and PC-based software in conjunction with their specific assay(s). This evaluation must include the confirmation that performance characteristics for the specific assay(s) are met. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... - Page 9 Use of labware other than described in this document can result in -CAUTION- positioning errors during program execution. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C...
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Page 10: Symbols
40 years. UK Conformity Assessed marking is a certification mark that indicates conformity with the applicable requirements for products sold within Great Britain. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... - Page 11 Preface Page 11 of 51 Temperature limit Humidity limitation Unique device identifier Model number Importer Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C...
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Page 12: Conformance To Standards
CAN/CSA C22.2 No. 61010-2-101 Safety Requirements for Electrical Equipment For Measurement, Control, and Laboratory Use – Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 13: Emc Information And Technical Description
20. Protected against solid foreign objects of 12.5 mm diameter and greater. No protection against water. Disposal Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 15: Installation
• Cuvette holder (“C” models) • Software and optional accessories per the sales order, unless shipped separately Models Part Number Touchscreen Cuvette Port Incubation Shaking EPOCH2NS-SI EPOCH2NSC-SI Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 16: Unpack The Box
Installation Page 16 of 51 EPOCH2TS-SI EPOCH2TSC-SI Unpack the Box Save the packaging, in case you need to ship the instrument for service/repair. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 17: Remove The Shipping Hardware
• Dust. Readings may be affected by extraneous particles in the microplate wells. A clean work area is necessary to ensure accurate readings. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 18: Prepare The Host Computer
3. Plug the rounded end of the power supply's cord into the power inlet. 4. Plug the other end of the power cord into an appropriate power receptacle. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 19: Install The Cuvette Holder
Technical Support. Other models: contact Technical Support. (Optional) Press the lighted blue button on the front of the reader to store the microplate carrier in its chamber. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 20: Establish Communication
If you remain unable to get Gen5 and the reader to communicate with each other, contact Technical Support. Verify Performance Refer to the Instrument Testing section for Absorbance Test procedures. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 21: Repackaging And Shipping Instructions
Using a screwdriver, reattach the shipping bracket to the carrier. If applicable, remove the cuvette holder and apply tape over the cuvette port to prevent contamination during shipment. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 22: Repackage The Instrument And Accessories
Place the accessory tray in the box. Place accessories in the tray as shown below: Close the box. Secure it with shipping tape. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 23: Getting Started
3. cuvette holder (if equipped) 4. power on/off 5. carrier in/out, status 6. USB port for printer 7. power inlet 8. USB port for host computer (if used) Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 24: Non-Touchscreen Models
3. power on/off 4. carrier in/out, status LED 5. power inlet 6. USB port for host computer Optional Printer Refer to the printer's manual for installation and setup instructions. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 25: Using The Touchscreen
Raw data, Delta OD, Blanked data). Instrument Run a system test, define date/time settings, enable output to a printer and/or flash drive, configure a Take3 plate, and control temperature. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 26: Confirm Or Set The Time And Date
• Raw: The raw measurement value for each well. • Delta OD: Applicable when a secondary wavelength is selected in an absorbance protocol. This is the calculated value for each well of the Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 27: Define And Start A Quick Endpoint Read
Start to run the protocol. When the read is finished, the results are displayed and ready for output Instrument > Output. according to the settings defined under Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 28: Define And Start A Standalone Shake
Check the Active box. Define a Set point from 18°C to 65°C (64.4°F to 149°F). Heating begins immediately. Move the plate carrier in, if it is currently out. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 29: Create And Save A Protocol
Use the onscreen keyboard to enter a name for the protocol, then tap Save. Note that the protocol name is limited to 18 characters. Set the Read Mode to Endpoint, Kinetic, or Spectral. Define the protocol parameters and then tap Save. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 30: Endpoint Protocols
When finished, tap Save. The protocol name is added to the Main Menu. If your Endpoint assay requires incubation, activate Temperature Control before reading the plate. From the Main Menu, select Instrument and tap the Temperature tab. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 31: Kinetic Protocols
Eject the plate when done. If unchecked, the plate will remain If applicable, check in the chamber after the last kinetic read. If the assay requires incubation: Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 32: Spectral Scan Protocols
10, measurements are taken at 200, 210, 220, and so on until the End WL is reached. The number of spectral data points cannot exceed 100. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 33: Run A Protocol
2 Kinetic/Spectral protocols that were run. The next set of saved results will overwrite the oldest set for the read mode only with your acknowledgment. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 34: Edit, Delete, Or Copy A Protocol
Tap the green button in the upper-right corner of the display. The results are printed and/or saved to the USB flash drive, depending on the read mode and defined output format. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 35: Run A Take3 Session
240–300 nm 230 nm 240–300 nm ssDNA 230 nm 240–300 nm 1 Abs@1cm = 260–320 nm 1 mg/mL 260–320 nm 13.7 260–320 nm Lysozyme 26.4 260–320 nm Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 36: Define And Align A Take3 Plate
Edit and enter the pathlength values that either came with the Take3 plate or were generated through the Pathlength Correction procedure in the Take3 User Manual. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 37: Run A Take3 Protocol
If the number of valid blank wells is sufficient for the assay, tap Approve, otherwise select Cancel and address the problem (e.g., clean the slides). Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 38: Gen5 Software
In the Gen5 Task Manager, select Experiment > Create using an existing protocol. Select the desired protocol and click OK. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... - Page 39 When the read is complete, measurement values appear in Gen5. Select the desired data set from the Data list. Select File > Save and give the experiment an identifying name. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C...
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Page 40: Maintenance
Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible. Wear protective gloves when handling contaminated instruments. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 41: Schedule
Exposed surfaces may be cleaned (not decontaminated) with a cloth moistened (not soaked) with water or water and a mild detergent. Turn off and unplug the instrument from the power supply. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 42: Decontamination
Prepare a disinfecting solution: an aqueous solution of 0.5% sodium hypochlorite (NaClO). If the effects of sodium hypochlorite are a concern, 70% isopropyl alcohol may be used. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... - Page 43 Use a clean, dry lint-free cloth to wipe all wet surfaces. Discard the used gloves and cloths, using a biohazard trash bag and an approved biohazard container. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C...
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Page 44: Instrument Testing
Enter your user name and other information (if desired), and then click OK. The results report appears. It should show “SYSTEM TEST PASS”. If it shows “SYSTEM TEST FAIL” contact Technical Support. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 45: Absorbance Plate Test
Place the Test Plate in the microplate carrier so that well is in the left-rear corner of the carrier (as you are facing the carrier). Click to run the test. Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... - Page 46 Check the filters on the Test Plate to ensure they are clean. If necessary, clean them with lens paper. Do not remove the filters from the test plate. Do not use alcohol or other cleaning agents. Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C...
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Page 47: Specifications
Operational: 10% to 85% relative humidity (non-condensing) Storage: 10% to 80% relative humidity (non-condensing) Power Supply 24-volt external power supply compatible with 100–240 V~; +/- 10% @50–60 Hz Power Consumption < 120W Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... -
Page 48: Read Specifications
Kinetic Measurements 96 Well Mode Delay Time 630 nm Normal 100 msec Rapid 0 msec Sweep Mode Delay Time 630 nm Normal 100 msec Rapid 0 msec Sweep Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C... -
Page 49: Optical Performance
384-well plate, normal read speed, 100-ms delay after plate movement 0.000–1.500 OD +/-1% +/-0.005 OD 1.500–2.000 OD +/-3% +/-0.005 OD 96-well and 384-well plate, sweep read speed 0.000–1.000 OD +/-2% +/-0.010 OD Agilent BioTek Epoch 2 Instructions for Use Agilent Techologies, Inc. 1771011I Revision C... - Page 50 Page 50 of 51 Optics 200 to 999 nm λ range ± 2 nm λ accuracy ± 0.2 nm λ repeatability < 5 nm λ bandpass Detector Photodiodes (2) Agilent BioTek Epoch 2 Instructions for Use Agilent Technologies, Inc. 1771011I Revision C...
- Page 51 Replaced the Notices section with Copyright information. Added Customer Care contact information. Removed the Warranty and Product Registration section. Added information for Getting Started with Gen5. Original Language – EN Manufactured by Agilent Technologies, Inc. 5301 Stevens Creek Blvd. Santa Clara, CA 95051...