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Agilent BioTek Epoch
Absorbance Microplate
Spectrophotometer
ERRATA NOTICE: This document
contains references to BioTek.
Please note that BioTek is now
Agilent. For more information, go to
www.agilent.com/lifesciences/biotek.
INSTRUCTIONS FOR USE
Document part number 7201009I
Agilent Technologies, Inc.
For In Vitro Diagnostic Use
Revision C
May 2022
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Summary of Contents for Agilent Technologies BioTek Epoch
- Page 1 Agilent BioTek Epoch Absorbance Microplate Spectrophotometer INSTRUCTIONS FOR USE ERRATA NOTICE: This document Document part number 7201009I contains references to BioTek. Revision C Please note that BioTek is now Agilent Technologies, Inc. Agilent. For more information, go to May 2022 www.agilent.com/lifesciences/biotek.
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Page 2: Table Of Contents
Prepare the Host Computer ................... 14 Connect the Host Computer and Reader ............... 15 Turn on the Reader ......................15 Establish Communication ....................15 Troubleshooting ..................... 15 Verify Performance ......................16 Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... - Page 3 Test Plate Certificates .................... 23 Define the Absorbance Test Plate Parameters ............24 Procedure ....................... 24 Specifications ........................25 General Specifications ....................25 Read Specifications......................26 Optical Performance ...................... 27 Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C...
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Page 5: Preface
Copyright All Rights Reserved © Agilent Technologies, Inc. 2021, 2022. No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. -
Page 6: Responsible Person (Ukrp)
Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 7: Warnings And Precautions
Sodium Hypochlorite. Do not expose any part of the instrument to the -CAUTION- recommended diluted sodium hypochlorite solution for more than 20 minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 8: Components
Use of labware other than described in this document can result in -CAUTION- positioning errors during program execution. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 9: Symbols
40 years. UK Conformity Assessed marking is a certification mark that indicates conformity with the applicable requirements for products sold within Great Britain. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... - Page 10 Preface Page 8 of 31 Temperature limit Humidity limitation Unique device identifier Model number Importer Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C...
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Page 11: Conformance To Standards
CAN/CSA C22.2 No. 61010-2-101 Safety Requirements for Electrical Equipment For Measurement, Control, and Laboratory Use – Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 12: Emc Information And Technical Description
20. Protected against solid foreign objects of 12.5 mm diameter and greater. No protection against water. Disposal Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 13: Installation
EPOCH-SI Microplate Spectrophotometer. EPOCHH-SI Microplate Spectrophotometer for high-altitude sites. EPOCHR-SI Microplate Spectrophotometer. BioStack and 3rd party compatible. EPOCHRH-SI Microplate Spectrophotometer for high-altitude sites. BioStack and 3rd party compatible. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 14: Unpack The Box
Unpack the Box User manual USB cable Power cable Power supply Take3 plate Gen5 software Save the packaging, in case you need to ship the instrument for service/repair. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 15: Remove The Shipping Hardware
The plug prevents light from entering the reading chamber during operation. Shipping screw and o-ring Plug Figure 1 Plug Shipping screw and o-ring Figure 2 Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 16: Select An Appropriate Location
• Ensure the computer meets the minimum system requirements as described in the Gen5 Instructions for Use. • You must have administrator privileges to install Gen5. Log in to Windows as “Administrator” or consult your IT department for assistance. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 17: Connect The Host Computer And Reader
If the communication attempt is not successful, try the following: • Is the reader connected to the power supply and turned on? • Is the communication cable firmly attached to both the reader and the computer? Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 18: Verify Performance
If you remain unable to get Gen5 and the reader to communicate with each other, contact Technical Support. Verify Performance Refer to the Instrument Testing section for Absorbance Test procedures. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 19: Repackaging And Shipping Instructions
Insert the plug in its original hole and use the screwdriver to re-install the shipping screw and o-ring in its original position. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 20: Repackage The Instrument And Accessories
Bundle the power cord and place it into the accessories box as shown. Place the power supply and USB cable in the accessories box. Close the top of the box and secure it with shipping tape. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 21: Getting Started
When the read is complete, measurement values appear in Gen5. Select the desired data set from the Data list. File > Save Select and give the experiment an identifying name. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 22: Maintenance
Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible. Wear protective gloves when handling contaminated instruments. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 23: Schedule
Required Materials • Sodium hypochlorite (NaClO) • 70% isopropyl alcohol (as an alternative to sodium hypochlorite) • Deionized or distilled water • Safety glasses Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 24: Procedure
Use a clean, dry lint-free cloth to wipe all wet surfaces. Discard the used gloves and cloths, using a biohazard trash bag and an approved biohazard container. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 25: Instrument Testing
NIST-traceable values. Test Plate Certificates To run this test on the Epoch, you will need the 7-Filter Absorbance Test Plate (Part Number 7260522) with its accompanying certificates. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 26: Define The Absorbance Test Plate Parameters
Check the filters on the Test Plate to ensure they are clean. If necessary, clean them with lens paper. Do not remove the filters from the test plate. Do not use alcohol or other cleaning agents. Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 27: Specifications
40° C (non-condensing) Storage: 10% to 80% relative humidity (non-condensing) Power Supply 24-volt external power supply compatible with 100–240 V~; +/- 10% @50–60 Hz Power Consumption < 40W Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 28: Read Specifications
Kinetic Measurements 96 Well Mode Delay Time 630 nm Normal 100 msec Rapid 0 msec Sweep Mode Delay Time 630 nm Normal 100 msec Rapid 0 msec Sweep Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... -
Page 29: Optical Performance
384-well plate, normal read speed, 100-ms delay after plate movement 0.000–1.500 OD +/-1% +/-0.005 OD 1.500–2.000 OD +/-3% +/-0.005 OD 96-well and 384-well plate, sweep read speed 0.000–1.000 OD +/-2% +/-0.010 OD Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C... - Page 30 Page 28 of 31 Optics 200 to 999 nm λ range ± 2 nm λ accuracy ± 0.2 nm λ repeatability < 5 nm λ bandpass Detector Photodiodes (2) Agilent BioTek Epoch Instructions for Use Agilent Technologies, Inc. 7201009I Revision C...
- Page 31 In Vitro Diagnostic Regulation. Replaced the Notices section with Copyright information. Added Customer Care contact information. Removed the Warranty and Product Registration section. Original Language – EN Manufactured by Agilent Technologies, Inc. 5301 Stevens Creek Blvd. Santa Clara, CA 95051...