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Getting Started
Getting Started
Gen5 software supports all Epoch reader models. This section provides brief instructions
for creating a protocol and running an experiment. For more information, refer to
publications provided with Gen5 and the Gen5 help system
In Gen5, a protocol contains instructions for controlling the reader and (optionally)
instructions for analyzing the data retrieved from the reader. At a minimum, a protocol
must specify the procedure you wish to run. After creating a protocol, create an
experiment that references the protocol. You will run the experiment to read plates and
analyze the data.
In the
Gen5 Task
1.
Open the
2.
OK.
Select a
Plate
3.
Gen5 stores measurements and other characteristics for individual plate types in a
database. It is essential that you select (or define) the plate type to match the assay
plate. Otherwise, results may be invalid. See the
Gen5 Help for instructions.
Add steps to the procedure for reading the plate. Click
4.
reader supports the defined steps, and then click OK.
Optionally, perform any of these steps to analyze and report the results:
5.
• Open the
standards to the plate.
• Open the
include Transformation,
Analysis.
• Create a report or export template via the
Select
File > Save
6.
In the
Gen5 Task
7.
protocol.
Select the desired protocol and click OK.
8.
Select a plate in the menu tree and click
9.
When the read is complete, measurement values appear in Gen5. Select the
10.
desired data set from the
File > Save
Select
11.
Agilent BioTek Epoch Instructions for Use
7201009I Revision C
Manager, select
Procedure
dialog. If prompted to select a reader, select
Type.
Plate Layout
dialog and assign blanks, samples, controls, and/or
Data Reduction
dialog to add data reduction steps. Categories
Well
and give the protocol an identifying name.
Manager, select
Data
and give the experiment an identifying name.
(Help > Help
Protocol > Create
"Plate Type
Analysis,
Curve
Analysis, and
Report/Export
Experiment > Create using an existing
.
list.
Page 19 of 31
Topics).
New.
Epoch
and click
Database" topic in the
Validate
to verify that the
Qualitative
Builders.
Agilent Technologies, Inc.
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