Priming With A Syringe - Agilent Technologies 1200 Series Manual

Priming with a Syringe

When opening capillary or tube fittings solvents may leak out.
WA R N I N G
The handling of toxic and hazardous solvents and reagents can hold health risks.
➔
Before using a new degasser or new tubings for the first time:
1 Prime all tubings with at least 30 ml of iso-propanol no matter whether the
If you are changing to a solvent that is immiscible with the solvent currently in the tubing
N O T E
continue as follows:
2 Replace the current solvent with adequate organic solvent (see table above),
3 Disconnect solvent outlet tube of the channel that is supposed to be primed
4 Connect syringe adapter to solvent outlet tube.
5 Push syringe adapter onto syringe.
6 Pull syringe plunger to draw at least 30 ml of solvent through degasser and
7 Replace the priming solvent with the new solvent of your choice.
8 Pull syringe plunger to draw at least 30 ml of solvent through degasser and
9 Disconnect syringe adapter from solvent tube.
10 Connect the solvent tube to the appropriate channel of the MCGV.
11 Repeat step 3 on page 45 to step 10 on page 45 for the other solvent
1200 Series Quarternary Pump User Manual
Please observe appropriate safety procedures (for example, goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
channels will be used with organic mobile phase or with water.
if current solvent is organic or with water, if current solvent is an inorganic
buffer or contains salt.
from your pump.
tubing.
tubing.
channels.
Installing the Pump
Priming and Purging the System
3
45