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Agilent Technologies 1200 Series Reference Manual

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Agilent 1200 Series
Manual Injector
Reference Manual
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  • Page 1 Agilent 1200 Series Manual Injector Reference Manual...
  • Page 2 (Computer Software) and, for Department of Defense purchases, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commercial Computer Software or Computer Software Documentation). If a federal government or other public sector Customer has a need for 1200 Series MI Reference Manual...
  • Page 3 Site requirements and installation of the manual injector Repairing the Manual Injector Instructions on simple, routine repair procedures Parts and Materials Detailed illustrations and lists for identification of parts and materials Theory of Operation Operation and mechanical hardware 1200 Series MI Reference Manual...
  • Page 4 1200 Series MI Reference Manual...

  • Page 5: Table Of Contents

    Repairing the Manual Injector Overview of Repair Procedures Flushing the Manual Injector Cleaning the Manual Injector Stator Face Injection-Valve Seal Position-Sensing Switch Parts and Materials Manual Injector Injection-Valve Assembly Theory of Operation Description Injecting Sample 1200 Series MI Reference Manual...
  • Page 6 Safety Information Solvent Information Agilent Technologies on Internet Index 1200 Series MI Reference Manual...

  • Page 7: Installing The Manual Injector

    Agilent 1200 Series Manual Injector Reference Manual Installing the Manual Injector Unpacking the Manual Injector Installing the Manual Injector Flow Connections Leak Drainage Choice of Injection Seal Needles Agilent Technologies...

  • Page 8: Unpacking The Manual Injector

    The delivery checklist is shown in Table 1. To aid in parts identification, please see “Parts and Materials” on page 29. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Table 1 Manual Injector Checklist Description Quantity Part Number...
  • Page 9 Installing the Manual Injector Table 1 Manual Injector Checklist (continued) Description Quantity Part Number Valve syringe, fixed needle 50 µl 5182-9619 Reference Manual G1328-90010 1200 Series MI Reference Manual...

  • Page 10: Installing The Manual Injector

    1 Place the baseplate on the bench. 2 Connect the two organizer plates to the base plate. Figure 1 Connecting the Organizer Plates 3 Screw the mounting pole into one of the two holes in the organizer plate. 1200 Series MI Reference Manual...
  • Page 11 Installing the Manual Injector 4 Slide the manual injector onto the mounting pole (see Figure 2). Tighten the mounting screw. Mounting screw Figure 2 Installing the Mounting Pole and Manual Injector 1200 Series MI Reference Manual...
  • Page 12 Installing the Manual Injector 5 Install other system modules on top of the manual injector baseplate (see Figure Figure 3 Installing the System 1200 Series MI Reference Manual...
  • Page 13 APG remote connector Start cable HPI B HP- I B A ddr e ss Position-sensor cable Figure 5 Figure 4 Installing the Start Cable 6 Connect the capillaries to the manual injector (see “Flow Connections” page 14). 1200 Series MI Reference Manual...

  • Page 14: Flow Connections

    The outlets of the two vent capillaries (ports 5 and 6) and the needle port must be at C A U T I O N the same level to prevent siphoning (see Figure 6 on page 15). 1200 Series MI Reference Manual...
  • Page 15 Sample loop Waste Vent tube Sample loop To column From pump outlet Figure 5 Flow Connections Vent capillaries (ports 5 and 6) Vent capillaries and needle port at the same level Figure 6 Vent Capillaries 1200 Series MI Reference Manual...

  • Page 16: Leak Drainage

    WA R N I N G leak, solvent will drop into the leak channel in the baseplate, from where it is channelled to the front and back of the baseplate. Leak channel Figure 7 Leak Drainage 1200 Series MI Reference Manual...

  • Page 17: Choice Of Injection Seal

    C A U T I O N The manual injector is not supplied with syringes or needles. Use needles with 0.028-inch outer diameter (22 gauge) × 2-inch long needle, º without electro-taper, and with 90 point style (square tip). 1200 Series MI Reference Manual...
  • Page 18 Installing the Manual Injector 1200 Series MI Reference Manual...

  • Page 19: Repairing The Manual Injector

    Agilent 1200 Series Manual Injector Reference Manual Repairing the Manual Injector Overview of Repair Procedures Flushing the Manual Injector Cleaning the Manual Injector Stator Face Injection-Valve Seal Position-Sensing Switch Agilent Technologies...

  • Page 20: Overview Of Repair Procedures

    24 performance shows indication of leakage or wear Exchanging the When cable damaged or when no 10 minutes “Position-Sensing Switch” position-sensing start signal is sent when switching to page 26 switch the inject position 1200 Series MI Reference Manual...

  • Page 21: Flushing The Manual Injector

    Needle-port Cleaner Cleaning the Manual Injector The manual injector base should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent. 1200 Series MI Reference Manual...

  • Page 22: Stator Face

    3 Insert the new stator face onto the stator head. 4 Install the stator head and stator face onto the valve. Ensure the pin in the stator ring is aligned with the hole in the stator head. 1200 Series MI Reference Manual...
  • Page 23 Repairing the Manual Injector 5 Secure the stator face and stator head in place with the stator screws. Tighten each screw alternately ¼-turn until the stator head is secure. 1200 Series MI Reference Manual...

  • Page 24: Injection-Valve Seal

    Parts required Rotor seal, 0100-0623 (Vespel™), 0100-0620(Tefzel™) 1 Loosen the three stator screws. 2 Remove the stator head and stator face (see “Stator Face” on page 22). 3 Remove the stator ring. 4 Remove the seal. 1200 Series MI Reference Manual...
  • Page 25 Ensure the pin in the stator ring is aligned with the hole in the stator head. 9 Secure the stator face and stator head in place with the stator screws. Tighten each screw alternately ¼-turn until the stator head is secure. 1200 Series MI Reference Manual...

  • Page 26: Position-Sensing Switch

    No start signal when switching to the inject position Tools required Hex key, 9/64 inch (supplied with valve) Parts required Position-sensing switch, 0490-1849 1 Loosen the three stator screws. 2 Remove the stator head and stator face (see “Stator Face” on page 22). 1200 Series MI Reference Manual...
  • Page 27 5 Insert the new sensing switch into the stator ring. 6 Install the stator ring. Ensure the pin in the stator ring is aligned with the hole in the valve body. 1200 Series MI Reference Manual...
  • Page 28 9 Secure the stator face and stator head in place with the 10 Reconnect the sensor cable to the start cable. stator screws. Tighten each screw alternately ¼-turn until the stator head is secure. 1200 Series MI Reference Manual...

  • Page 29: Parts And Materials

    Agilent 1200 Series Manual Injector Reference Manual Parts and Materials Manual Injector Injection-Valve Assembly Agilent Technologies...

  • Page 30: Manual Injector

    5001-3738 Base plate G1328-44111 Organizer plate 5042-8553 Catch tube cap 5042-8576 Name plate for Agilent 1200 Series 5042-8901 Valve syringe, fixed needle 50 µl 5182-9619 Connection capillary, 0.17 mm id, 500 mm (not shown) G1328-87600 Start cable (not shown) 0100-1677...
  • Page 31 Parts and Materials Figure 9 Manual Injector 1200 Series MI Reference Manual...

  • Page 32: Injection-Valve Assembly

    0100-1857 Rotor seal (Vespel™) 0100-0623 Rotor seal (Tefzel™) 0100-0620 Stator face 0100-1859 Stator head 0100-1860 Stator screws 0100-1861 Hex key 9/64 inch (for stator screws — not shown) 8710-0060 Position sensing switch (not shown) 0490-1849 1200 Series MI Reference Manual...
  • Page 33 Parts and Materials Figure 10 Injection-Valve Assembly 1200 Series MI Reference Manual...
  • Page 34 Parts and Materials 1200 Series MI Reference Manual...

  • Page 35: Theory Of Operation

    Agilent 1200 Series Manual Injector Reference Manual Theory of Operation Description Agilent Technologies...

  • Page 36: Description

    14). The valve is mounted on a steel mounting pole, and can be installed at the left- or right-hand side of the LC system. Mounting pole Injection port Figure 11 Rheodyne 7725i Injection Valve 1200 Series MI Reference Manual...

  • Page 37: Injecting Sample

    The sample fills the loop, and excess sample is expelled through the vent tube connected to port 6. Pump Sample loop Column Needle port Figure 12 LOAD Position 1200 Series MI Reference Manual...
  • Page 38 (ports 1 and 2 connected). All of the sample is washed out of the loop onto the column. The needle port is connected to the vent tube (port 5). Pump Column Sample loop Needle port Figure 13 INJECT Position 1200 Series MI Reference Manual...
  • Page 39 Agilent 1200 Series Manual Injector Reference Manual Safety Information Solvent Information Agilent Technologies on Internet Agilent Technologies...
  • Page 40 • Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1-% solution of acetic acid in methanol may attack steel. • Mixtures of carbon tetrachloride with 2-propanol or THF. dissolve stainless steel. 1200 Series MI Reference Manual...
  • Page 41 Safety Information Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the internet at: http://www.chem.agilent.com 1200 Series MI Reference Manual...
  • Page 42 Safety Information 1200 Series MI Reference Manual...
  • Page 43 Rheodyne 7725i, information on internet, INJECT, 36, salt solutions, injecting sample, sample loops, injection seal, sample volume, tefzel, solvents, 1200 Series MI Reference Manual...
  • Page 44 Index 1200 Series MI Reference Manual...
  • Page 46 In This Book This manual contains technical reference information about the Agilent 1200 Series manual injector. The manual describes the following: • installing the manual injector, • repairing the manual injector, • parts and materials, and • theory of operation.

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