Refractive Index Calibration - Agilent Technologies InfinityLab LC Series User Manual

8 Test Functions

Refractive Index Calibration

Refractive Index Calibration

Refractive Index Calibration
The refractive index calibration is based on a sucrose calibration solution, which
has a known refractive index compared to LC grade water. After both the sample
and reference cells have been purged with LC grade water the sucrose solution is
introduced into the flow cell and then the built-in refractive index calibration
functionality is used.
Filling the sample cell with the sucrose calibration solution will give a theoretical
detector response of 512,000 nRIU +/- 5,000 nRIU. The calibration algorithm will
allow the actual detector response, if different, to be changed to the theoretical
value.
Refractive index calibration is only required after exchange of the optical unit or
NOTE
the main (RIM) - board.
Agilent InfinityLab LC Series RID User Manual 108