Transmitted-Light Stand, Bright And Dark Field; Transmitted-Light Stand Hl - Leica MS5 User Manual

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Transmitted-light stand, bright and dark field
The illumin ation of the transmitted-light stand bright and dark
fie ld consists of a fibe r-optic light guide and a light source.
.1.\.
Observe the safety regulations from the
~ manufacturer of the light source.
..,. Unplug the f iber-optic light guide from t he con11ection in
the stand .
..,. Fit the side-faced column to the t ransmitted- light base as
described on page 23 .
..,. Fit the microscope carrier and stereom icroscope as
described on page 34 .
..,. Attach the fibe r-optic light guide at t he stand and the
light source .
Starting
up
Before using the light source, read the correspo nding use r
manual.
Transmitted light.
bright field
Transmitted light,
dark field
Bright field is suitable for examining translucent objects featur-
ing contrasting structures. The object is di rect ly illum inated
from be low and is see n in its natural colo rs against a bright
background .
..,. Slide handle to the right until the
stop: bright field .
Dark field offers mo re information for objects poor in cont rast
with rather poorly marked or very fine structures. In this case,
the light beams penetrate the object at a flat angle . Fine detail
structures and contours contrast brilliantly and brightly from
dark background.
..,. Slide the handle out to the right:
dark fie ld.
Leica M Stereomicro scopes - Assembly
Transmitted-light stand HL
Column-+ Base The column seat contains Alien screws.
..,. Remove the glass stage plate .
..,. Invert the transmitted-light base and place it on a table .
..,. Hold column against the column seat from underneath .
..,. Insert the Alien screws into the co lumn from the top and
tig hten them .
..,. Invert the transmitted -light base and insert the glass
stage plate .
..,. Fit additional components such as the microscope carrier
and optics carrier (page 34) .
Illumination Add a very luminous cold light source and a light guide with
Accessory condenser
-+Basis
Use
Tilted mirror
a channel to the transmitted-light stand (TVC, active
0 9
mm).
..,. Insert light guide into the base at the rear.
..,. Connect light guide to the light source.
For stereomicroscopes with hig h apertures such as Leica
MZ12s, MZ16 and MZ16 A with objective 1.6x, the resolution
can be increased by inserting an accessory condenser.
..,. Push the accessory condenser into the lens holder at the
underside of the transmitted-light base and secure it with
2 Alien screws.
..,. Turn on the light source according to the manufacturer's
user manual and adjust the light intensity .
..,. Place a transparent object on the glass stage plate and
focus.
The built-in mirror features a clear and an obscured side and
can be rotated and moved. The obscured side provides a dif-
fused illumination . The angle of in cidence in the specimen
plane changes depending on the position of the mirror so that
the transmitted light bright field via oblique illumination can also
generate an illumination similar to dark field.
..,. Rotate and move the mi rror w ith the black rotary knob on the
rig ht side .
..,. Adjust exact bright fie ld and maximum illumination: move
mi rror to the stand column until it stops.
Rotate the mirror unti l it engages in the 45° posi t ion.
Le ica
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Stereomicroscopes - A ssembly 27