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Summary of Contents for Leica MacroFluo
- Page 1 Leica MacroFluo ™ User manual...
- Page 2 Thank you for your faith in our product. We hope your work with it is both enjoyable and successful. Leica MacroFluo™ with zoom 6.3:1 or 16:1 are worldwide the only macro docu- mentation systems for fluorescence. The MacroFluo™ concept combines the advantages of macroscopy –...
- Page 3 Overview of the instrument Fig. 1 Leica MacroFluo™ with zoom system 16:1 1 Base of stand (transmitted-light base HL RC™ 8 Coaxial incident light fluorescence illuminator shown) LRF 4/22 2 Focusing drive with column (shown with motor 9 Lamp housing 106 z with 100 W high-pressure...
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Page 4: Table Of Contents
3.6 Microscopy with/without glasses ..21 3.7 Adjusting diopter settings ....22 Leica MacroFluo – User manual... -
Page 5: Safety Concept
These operating instructions are an essential component of the instrument. Carefully read these operating instructions and those listed Included with your Leica MacroFluo™ is an inter- above before assembly, startup and use. In partic- active CD-ROM with all pertinent user instructions ular, please observe all safety instructions. -
Page 6: Symbols Used
1.2 Safety instructions Description Warning of a danger This symbol indicates especially important infor- Leica MacroFluo™ systems are macroscopes mation that, if not observed, with vertical beam path for precise fluorescence – can cause hazards to personnel, applications. Leica MacroFluo™ systems are –... - Page 7 Permitted uses Place of use The Leica MacroFluo™ allows for examining, – The Leica MacroFluo™ may only be operated manipulating, sorting and documenting entire in closed, dust-free rooms at +10 ˚C to +40 ˚C. unprepared fluorescent objects. The high resolu- –...
- Page 8 Integration in third-party products The ergonomic optics concept and the design of When installing Leica products into third-party Leica instruments aim to limit the strain on the products, note the following: The manufacturer of user to the lowest possible level.
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Page 9: Light Source: Safety Instructions
1.3 Light source: Safety instructions Leica MacroFluo™ with fluorescence illuminator Protective measures of the manufacturer: may be used only with the Leica lamp housing – UV protection screen in front of the object 106 z for 100-W mercury bulb, supply unit and plane prevents direct view onto the UV beams. -
Page 10: Assembly
10 447 185 (500 mm) 10 446 100 (500mm) 10 445 154 10 447 041 (500mm) 10 399 211 10 445 631 10 445 654 10 445 363 10 445 387 10 445 367 10 446 359 Leica MacroFluo – Assembly... -
Page 11: Assembly Sequence
M2-267-1 operat- ing stop, glass stage plate or optional spacial ing instructions, in particular the safety instruc- stage (gliding stage, Leica MATS heating stage) tions. – carrier for the incident illuminator Fig. 2 see p. 12 –... - Page 12 14 Wide-angle HC plan eyepieces 10×/22 or 10×/25 focus) 15 TV adapter of your choice 5 Gliding stage (optional) with glass stage plate 16 CCD camera of your choice (Leica DFC300 FX 6 Zoom system (Z6 APO shown) shown) 7 Objective of your choice...
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Page 13: Trinocular Tube
2.5 Trinocular tube 2.6 Zoom system Trinocular tube ➜ incident illuminator The MacroFluo™ can be combined with a 6:1 (Z6 APO) or 16:1 (Z16 APO) zoom system. Tool: Allen key. Zoom system ➜ Carrier for incident Slightly unscrew the hexagon-head screw (3.9). -
Page 14: Objectives
(see assem- bly diagram p. 10). Always use the HR objective with fine focusing. – The Leica M series 0.63×, 0.5×, 0.32× achromatic objectives, which can be attached to the diaphragm ring of the zoom system or the fine Fig. -
Page 15: Uv Protection Screen
1 Hinged arm light spot (see p. 9). 2 Clamp with clamping screw for fastening to carrier 3 Central rotary knob 4 Clamp with clamping screw for fastening to UV protection screen 5 UV protection screen Leica MacroFluo – Assembly... -
Page 16: Lamp Housing
2.9 Lamp housing 2.10 Instrumentation of nosepiece disc The Leica lamp housing 106 z (11 504 069) is the light The nosepiece disc can be fitted with a total of source for the incident illuminator LRF 4/22. It is 4 filter blocks (filter system). At present, 19 fluo- used with 100-W mercury burners (11 500 138) or rescence blocks (p. - Page 17 Never look into the direct incident light beam path! Fig. 8 Self-adhesive labels and nosepiece disc Fig. 6 Incident light fluorescence illuminator 1 Cover of nosepiece slot 2 Recess for placing a finger 3 Nosepiece disc for changing filter blocks Leica MacroFluo – Assembly...
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Page 18: Connection Of Digital Or Video Cameras
Structure of digital camera with Vario TV adapter 1 Photo mount on the trinocular tube 2 Vario TV adapter 3 Leica digital camera 4 Clamping screw for fastening the adjustment option of the Vario TV adapter Leica MacroFluo – Assembly... -
Page 19: Operation
We recommend that you familiarize your- self with the operation of your Leica MacroFluo™ systems, such as adjusting the parfocality, zoom, 3.1.3 Disconnecting from the power supply focusing drive, eyebase, etc. in transmitted light and to experiment with it. -
Page 20: Zoom
There are certain switchable zoom positions which can be activated for repetitive tasks: – For the Leica Z6 APO, these are 0.57 / 0.8 / 1 / 1.25 / 1.6 / 2 / 2.5 / 3.2 / 3.6. – For the Leica Z16 APO, these are 0.57 / 0.8 / 1 / 1.25 / 1.6 / 2 / 2.5 / 3.2 / 4 / 5 /6.3 / 8 / 9.2... -
Page 21: Iris Diaphragm
Hold the left or right fine drive (12.6b). correct position of the eye lens for normal eye- Turn the other fine drive until the desired resis- sight and for users wearing glasses during tance is reached during focusing. microscopy work with glasses. Leica MacroFluo – Operation... -
Page 22: Adjusting Diopter Settings
Fully OPEN the iris diaphragm (13.6). Turn the eye lenses (13.2) until the rotating part reaches the line engraved in the eyepiece housing. While adjusting the eye lenses, securely hold the eyepiece at the bottom of the knurled ring. Leica MacroFluo – Operation... - Page 23 1 Adjustable tubes for adjusting the eyebase 2 Eye lenses for adjusting the diopter settings 3 Adjustable eyecups 4 Working distance 5 Iris diaphragm 6 Knurled ring for zoom adjustments 7 Focusing drive (motor focus shown) Leica MacroFluo – Operation...
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Page 24: Incident Light Fluorescence Illuminator
3 = open The illumination is coupled into the zoom optics. To protect sensitive fluorescence specimens This unique innovation from Leica Microsystems Restrict the field diaphragm (14.2) as needed. guarantees exact light guide, maximum light utilization and homogenous fields of view in any Centering the field diaphragm zoom position. -
Page 25: Aperture Diaphragm
The BG38 filter prevents a slight red background in the image. If this does not pose a problem, the BG38 filter (14.5) can be swung out to use the full lamp power for exciting the fluores- cence. Leica MacroFluo – Operation... -
Page 26: Appendix
(with filters from other manufactur- Specifically for multi-parameter fluorescence ers) so that they meet the requirements according to 0-pixel shift (ch. 3.8). This requires that the third-party products meet for customer-specific instrumentation certain minimum quality requirements. Leica FluoCombi III™ – Appendix... -
Page 27: Assignment Of Fluorochromes
– Bisbenzimide (Hoechst) A, D – Blankophor BA A, D, H 3 – Blankophor SV – BODIPY FL L 5, K 3, I 3 – Brilliant sulfoflavine FF D, H 3 – Bromobimane (Thiolyte) – Calcein Leica MacroFluo – Appendix... - Page 28 – Fast blue – Fast green FC G N 2.1, M 2 – Feulgen N 2.1, TX 2 – FDA (FluoresceinDiAcetate) I 3, H 3, K 3, L 5 – FIF (Formaldehyde induced fluorescence) D, A Leica MacroFluo – Appendix...
- Page 29 – Nuclear Fast Red N 2.1, M 2, N 3 – Nuclear Yellow – Olyvomycine – Oregon Green (488, 500, 514) – Oxytetracycline – Pararosaniline (Feulgen) N 2.1, TX 2 – Phosphine 3 R I 3, H 3 Leica MacroFluo – Appendix...
- Page 30 – TRITC (Tetramethyl rhodamine Isothiocyanat) N 2.1, N 3 – TRITC (selective) – True Blue – Uranine B – Uvitex 2 B A, D – XRITC N 2.1, N 3 – Xylene-orange N 2.1, M 2 Leica MacroFluo – Appendix...
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Page 31: Optical Data
6.25 62.5 78.8 39.4 1.25 16:1 57.5 Data with Leica DFC300 FX Full Frame digital camera Tube factor: 1.25× trinocular tube FSA HC L 3TP Video objective 0.63× Iris diaphragm open Objectives Planapo 1× Planapo 2× Planapo 5× / 0.5 Planapo 0.5×... -
Page 32: Technical Data, Performance
4.4 Technical data, performance characteristics Designation Leica MacroFluo™ with 6.3 : 1 zoom Leica MacroFluo™ with 16 : 1 zoom Microscope type Macroscope with vertical beam path, apochromatic zoom system, Incident illuminator, fluorescence illumination coupled into the zoom optics, and trinocular tube Zoom system Apochromatic zoom 6.3 : 1... - Page 33 Module system Imaging Image recording/storage Various digital FireWire camera systems for fluorescence and processing Software for image archiving, Leica Image Manager with Image Overlay modules analysis and processing and multifocus, FW4000, QWin Video objectives – 0,35×, 0,5×, 0,63, 1× with C-mount –...
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Page 34: Dimensions
4.5 Dimensions Dimensions in mm Leica MacroFluo – Appendix... - Page 35 ∅58 ∅58 ∅58 37.2 ∅58 37.2 ∅63 ∅63 Leica Z16 APO Leica Z6 APO Leica MacroFluo – Appendix...
- Page 36 ∅45 ∅45 M42x3 M42x3 ∅58 ∅45 M42x3 ∅58 ∅45 M42x3 ∅58 ∅45 (Fine Focus Interface) ∅38 ∅58 (Achromat Objective Interface) ∅54 Adapter for achromatic objectives Leica MacroFluo – Appendix...
- Page 38 Leica Microsystems – the brand for outstanding products Leica Microsystems’ mission is to be the world’s first-choice provider of innovative The companies of the Leica Microsystems Group operate internationally in four business solutions to our customers’ needs for vision, measurement, lithography and analysis segments, where we rank with the market of microstructures.
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