Leica M-Series User Manual

Leica M-Series User Manual

Stereomicroscopes

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Leica M-Series
Stereo-
microscopes
User Manual

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Summary of Contents for Leica M-Series

  • Page 1 Leica M-Series Stereo- microscopes User Manual...
  • Page 2: Table Of Contents

    Cold-light source Leica CLS Series ........
  • Page 3 Page Accessories Fitting accessory tubes ..........33 Double-iris diaphragm .
  • Page 4: Safety Concept

    ErgoTubus™, ErgoKeil™ 5°–25°, the swinging-arm stand service. Customer service is a big thing with us. Not only before ESD and the cold light sources Leica L2 and CLS consist of ESD- the sale, but afterwards as well.* discharging material (surface resistance (<10 ohms/squared, discharge time <2 seconds, 1,000V to 100V).
  • Page 5: Symbols

    Liquids Conformity with European Community directive Be careful when handling liquids. If spilt on the equipment, they can: The electrically-operated accessories for the Leica MS5, MZ6, , MZ9 , MZ12 and MZAPO stereomicroscopes are – cause the stereomicroscopes and accessories to become...
  • Page 6: Controls And Functions

    9 Clamping screws section (see contents list on page 2). These hold the eyepieces in the binocular tube. Leica MZAPO see page 8. 10 ErgoTube™ Stereomicroscope The viewing angle is adjustable within the range 10° – 50°.
  • Page 7 MZ12...
  • Page 8: Short Instructions

    Short instructions Inspect the cleanliness Remove dust and dirt from the instrument (page 39). Checklist Clean: Optical accessories, glass stage plates, and any graticules used (page 39). In this overview Only for variable ErgoModules™ we have summarized the working sequence. You will find detailed user instructions on the pages indicated.
  • Page 9: Use

    45° tube are the differing heights of the outfits, the various accessories and working distances, and the fact that the same instrument is often used by several people of differing builds. This problem can be effectively solved by using the Leica Locating the eyepoint ErgoModules™.
  • Page 10: Changing The Magnification

    Scale of magnifications Magnification changer MS5, MZ6 MS5: 5 steps Display of magnification factors from 0.63 – 4. MZ6: 6:1 zoom : 7.9:1 zoom : 9.5:1 zoom Display of magnification factors from 0.63 – 5. For other objective- MZ12 : 12.5:1 zoom eyepiece combinations, appropriate adhesive scales can be MZAPO: 10:1 zoom applied to the rotary knobs (page 19).
  • Page 11: Focusing

    When the MZ12 /MZAPO planachromatic and planapochromatic Does the focusing drive run too loosely or too tightly, or does objectives are used on the Leica MS5, MZ6, MZ7 and MZ9 the equipment drift downwards? the magnification is increased by the factor 1.25 . This factor You can match the ease of movement to the weight of the outfit is taken into account in the tables.
  • Page 12: Stereoscopic And Axial Observation

    This microscope carrier permits only stereoscopic observation, to the focusing drive (see page 18). using both beam paths. For the Leica MZ12 and Leica MZAPO, use the spacer ring • The optics carrier can be turned sideways in the microscope (page 16).
  • Page 13 The following adjustments must be carried out only once C Adjust the dioptric settings by each user. 6. Lowest magnification The procedures for adjusting the dioptric setting and the Engage the lowest magnification parfocality are somewhat different if an eyepiece contains a position.
  • Page 14: Working With The Stereomicroscope

    • When focusing on a fresh object, always start with the lowest magnification, because the larger field of view makes the object easier to find. Afterwards, select the magnification Working with the stereomicroscope you want. Place the object beneath the In this section objective.
  • Page 15: Assembly

    Assembly Basic outfit The components 1a Incident-light base with stage plate 1b Transmitted-light base with glass stage plate 2a Side-faced column with drive housing, coarse/fine 2b Side-faced column with drive housing 3 Microscope carrier 4 Optics carrier 5 Interchangeable objective 6 Binocular tube 7 Wide-field eyepiece for spectacle wearers, with eyecups 8 Various ErgoModules™...
  • Page 16: Spacer Rings

    /MZAPO if a spacer ring is fitted. • When the MZ12 /MZAPO planachromatic and planapo- chromatic objectives are used on the Leica MS5, MZ6, MZ7 and MZ9 stereomicroscopes, the magnification is increased by a factor of 1.25 . 10 445 821 MZAPO...
  • Page 17 Objective combinations Article no. MZ12 MZAPO Achromat Achromat 1 10 411 589 Achromat 1.5 10 422 562 Achromat 2 10 422 561 Achromat 0.8 10 473 832 Achromat 0.63 10 445 201 Achromat 0.5 10 422 563 Achromat 0.32 10 422 564 Plan Plan 1 10 446 275...
  • Page 18: Fitting The Column To The Incident- And Transmitted-Light Bases

    The combination of the optics carrier with the inclinable are all described in the appropriate sections (see contents list drive housing is described on page 22. on page 3). The Leica motor focus system is described in a separate user Side-faced column incident-light base manual.
  • Page 19: Binocular Tubes, Optics Carrier, Optical Accessories

    (page 16). These rings are fitted Tighten the clamping screw. and removed using the tool provided. • The Leica MZ9 is supplied with a spacer ring for the achromatic objectives and for the 1.0 planachromatic objective. When the ring has been removed by means...
  • Page 20: Graticules

    Graticules Stands • The graticules for measuring and counting are in mounts which can be fitted directly into the eyepieces. Transmitted-light stand, bright field, 20W • A highly-precise stage micrometer with a 50mm scale and Follow the safety instructions given 0.1mm and 0.01mm divisions enables the graticule to be on page 4.
  • Page 21 Changing fuses Operating the transmitted-light stand Use: Transmitted light (bright field), or inclined transmitted light – Two 160mAT fuses for voltages between 200–240V. Switch on transmitted light. – Two 315mAT fuses for voltages between 100–120V (see section “Starting up”, page 20). Disconnect the power cable Transmitted light, bright field from the stand.
  • Page 22: Transmitted-Light Stands, Bright And Dark Field

    Assembly Swinging-arm stand ESD The swinging-arm stand ESD is of conducting material (surface resistance 10 Ohm/square, discharge time <2 seconds Transmitted-light stand for bright and dark field from 1000V to 100V). Two sockets for connecting 4mm-diameter The illuminator of the transmitted-light stand for bright and dark ground cables are provided on the base.
  • Page 23 Horizontal arm cross member Possibilities for adjustment Slide the horizontal arm into the cross-member with the Tilting connecting piece towards you. Release the clamping screw. Tighten the clamping screw. Tilt the instrument. Retighten the clamping screw. Drive housing horizontal arm •...
  • Page 24: Large Swinging-Arm And Table-Clamp Stands

    Assembly Drive housing horizontal arm Here follow directions for fitting the drive with side-faced carrier Large swinging-arm and table-clamp stands rod. To fit the drive housing with peg, refer to page 22. Release the clamping lever. Table-clamp stand: Use the Tilt the carrier rod downwards.
  • Page 25 Possibilities for adjustment Tilting Release the clamping lever. Tilt the instrument. Retighten the clamping lever. Focusing with coarse/fine drive Set the distance approximately with the larger knob. Fine-focus with the smaller (concentric) knob. Using the ring on the right-hand drive knob, adjust the ease of movement of the drive.
  • Page 26: Illuminators

    Illuminators Transformers Follow the safety instructions given on page 4. Leica transformers may only be opened by authorized service personnel, and the power cable must be disconnected beforehand. The transformers are not to be used outdoors. Regulating transformer The 0–7V/40VA transformer powers the: –...
  • Page 27: Leica L2 Cold-Light Source

    The antistatic Leica L2 cold-light source is powerful, small, com- shines. pact, inexpensive and is suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes. In addition to one or two-arm light guides, corresponding accessories are also available for the coaxial, vertical and transmitted-light illumina- tion methods.
  • Page 28: Fibre-Optic Light Guide

    Cold light source Leica CLS series housing. The Leica CLS series is a high-performance line for high light intensity within the smallest possible space and flicker-free white light with the lowest possible thermal effect on the objects.
  • Page 29 Lampholder objective Operating the lamp Remove the objective. Follow the safety instructions given on page 4. Follow the directions for using the transformer Slide the adapter over the objective. (page 26). Using the connector, attach the lampholder to the adapter. Connect the cable between the lamp housing and the trans- former (page 26).
  • Page 30: Incident Lamp 6V/20W

    Illuminators Diffusing and other filters • A total of 4 filterholders with 32mm diameter filters can be 6V/20W incident lamp attached. A heat-absorbing filter is built in. • The 6V/20W lamp is an inclined incident source for illumi- Push the filterholder, complete with diffusing filter, on to one of nating spatial objects.
  • Page 31 Centring the bulb Hold the lamp housing and release the clamping screw. The 6V/20W bulb is a powerful source of illumination. Align the light spot with the The more carefully you set it up, the more uniform will be the object.
  • Page 32: Coaxial Illuminator

    100mm, 150mm, 175mm and the object. 200mm. • Spacer rings are required for the Leica MZ7 , MZ9 , MZ12 and MZAPO in order to secure the near-vertical illuminator to the optics carrier (page 16).
  • Page 33: Fitting Accessory Tubes

    Accessories Drawing tube • The drawing tube is for both left-handers and right-handers. Double-iris diaphragm, drawing tube, Fit the mirror on the left- or right-hand side of the optics carrier. discussion tube Fit the drawing tube between the optics carrier and the binocular Fitting tube as described on the left of •...
  • Page 34: Video-Phototubes: Photography, Video, Tv, Filming, Digital Imaging

    3-chip 2/3” 3-chip 1/2” 1” C-mount, CCD cameras MPS 30 / MPS 60 B-mount B-mount C-mount camera housings Leica DC cameras Photoautomats CCD camera CCD camera CCD camera 11 543 703 11 543 706 11 543 702 Adapter 11 541 038...
  • Page 35 As shown in the set-up diagram on page 34, there are various • Video-/phototubes are designed to support modular possibilities for adapting an MPS, video, digital or SLR camera. photomicrography systems, and Leica digital, video, film or SLR cameras. Example: •...
  • Page 36 Fit the video objective (2) on a Move lever to the left. Leica DC digital camera (3) with the C-mount connection. Connect the video objective with the mounted Leica DC digital camera on the right output of the video/phototube.
  • Page 37: Attachment For Vertical And Oblique Observation

    120mm port. its adapter ring directly into the optics carrier. Place the cup stage on it. • Spacer rings are required for the Leica MZ7 , MZ9 , MZ12 and MZAPO in order to secure the attachment for vertical and oblique observation to the optics carrier (page 16).
  • Page 38: Tips And Hints: What To Do If

    Notes Unsharp photographs. Remedy: Tips and hints: What to do if … • Focus precisely (page 11). • Adjust your dioptric settings exactly as described (page 12). The field of view is partly shadowed. Remedy: Colour photographs have a yellow cast. •...
  • Page 39: Care And Maintenance Of The Stereomicroscope, Maintenance

    If, contrary to expectations, your used, protect them against dust. stereomicroscope develops a fault, please call in a specialist – contact your local Leica agent or Leica Microsystems Ltd., Cleaning polymer components Heerbrugg, Switzerland. Some components are made of polymer, or are polymer-coated.
  • Page 40: Calculating Total Magnification And Field Diameter

    Notes Total magnification and field diameter Magnification of objective Magnification of eyepiece Magnification changer position Tube factor, e.g. 1.5 for coaxial incident light, 45° Ergotube™ 1.6 Factor 1.25 if the planachromatic and planapochromatic objectives of the MZ12 /MZAPO are used on the MS5, MZ6, MZ7 or MZ9 N FOV Field number of eyepiece.
  • Page 41: Optical Data, Ms5, Mz6

    Optical data, Leica MS5, MZ6 0.32 Achromat 0.5 Achromat 1.5 Achromat 1.0 Plan 1.0 Planapo 0.63 Planapo 0.5 Plan 1.6 Planapo Objective 1.0 Achromat 0.8 Achromat 0.63 Achromat 2.0 Achromat 0.8 Plan Working distance 81 Plan 55 Planapo 19 Planapo...
  • Page 42: Optical Data, Mz7

    Optical data, Leica MZ7 , MZ9 1.0 Plan 1.6 Planapo 0.63 Planapo 0.5 Plan 1.0 Planapo 0.32 Achromat 0.5 Achromat 1.5 Achromat Objective 1.0 Achromat 2.0 Achromat 0.8 Achromat 0.63 Achromat Working distance 81 Plan 55 Planapo 19 Planapo 97 Planapo...
  • Page 43: Optical Data, Mz12

    Optical data. Leica MZ12 , MZAPO 1.0 Plan 0.5 Plan 0.63 Planapo 0.8 Plan 1.6 Planapo Objective 1.0 Planapo Working distance 112 Plan 60 Plan 135 Plan 97 Planapo 19 Planapo 55 Planapo Eyepiece 26.3 52.5 41.2 32.8 12.8 16.4 10.0...
  • Page 44: Dimensions

    Dimensions, Leica MS5 with incident-light stand with transmitted-light stand 1.0x Achromat 1.0x Achromat Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 45 Dimensions, Leica MZ6 with incident-light stand with transmitted-light stand 1.0x Achromat 1.0x Achromat Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 46 Dimensions, Leica MZ7 with incident-light stand with transmitted-light stand 1.0x Plan 1.0x Plan Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 47 Dimensions, Leica MZ9 with incident-light stand with transmitted-light stand 1.0x Plan 1.0x Plan Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 48 Dimensions, Leica MZ12 with large incident-light stand with transmitted-light stand 1.0x Plan 1.0x Plan MZ12 MZ12 Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 49 Dimensions, Leica MZAPO with incident-light stand with transmitted-light stand 1.0x Planapo 1.0x Planapo MZAPO MZAPO Additional dimensional diagrams involving Leica ErgoModules™ are available from your Leica agency.
  • Page 50 Leica Microsystems – the brand for outstanding products Leica Microsystems’ Mission is to be the world’s first-choice provider of innovative The companies of the Leica Microsystems solutions to our customers’ needs for vision, measurement, lithography and analysis Group operate internationally in five business of microstructures.

This manual is also suitable for:

Ms5Mz6Mz75Mz95Mz125Mzapo

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