Chapter 6 Troubleshooting; Optical - Nikon Eclipse 50i Instructions Manual

6
Troubleshooting
If the microscope does not function property, take appropriate action as described below. If the
problem is still not resolved after referring to "Troubleshooting," please contact your nearest
Nikon representative

Optical

6.1
Problem
Field of view vignetting
Uneven illumination
across the field of view
Field of view not visible
Dirt or dust in the field
of view
Poor image quality
Poor contrast
Poor resolution
Uneven focus
Image is in motion.
Yellow-tinged
Field of view too bright
Field of view too dark
Possible causes
Parts installed incorrectly
Movable parts not switched correctly
Field diaphragm image not focused
on the specimen surface
Field diaphragm stopped down too
far
Dirt or dust on lens and container
Dirt or dust on lens and container
Field diaphragm image not focused
on the specimen surface
Dirt or dust on lens and container
Objective's correction ring not
matched to the thickness of the
bottom plate container.
Field diaphragm image not focused
on the specimen surface
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Specimen tilted relative to stage
surface.
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Lamp voltage too low (for 50i)
No ND filter in optical path
Lamp voltage too high (for 50i)
Condenser aperture diaphragm
stopped down too far
Field diaphragm image not focused
on the specimen surface
Optical path switchover dial not set
to 100% eyepiece
74
Remedy
Install the Parts (nosepiece, condenser,
etc.) correctly.
Rotate the parts (e.g., optical path
switchover dial, nosepiece , filter cube
switchover dial) until you feel resistance.
Focus and center the condenser.
Open the field diaphragm slightly wider
than the field of view.
Clean. Use a clean container.
Clean. Use a clean container.
Focus and center the condenser.
Clean. Use a clean container.
Adjust the ring correctly.
Focus and center the condenser.
Install correctly and rotate to the click
stop position.
Correctly reposition specimen on stage.
Install correctly and rotate to the click
stop position.
Adjust the brightness adjustment dial to
match the lamp ratings.
Place filter in optical path.
Reduce the voltage with the brightness
adjustment dial.
Should normally be set to 70 to 80% of
the objective N.A.
Focus and center the condenser.
Switch to 100% eyepiece.