Image Capture; Adjusting Light Intensity; Adjusting The Condenser; Confirming The Photomicrographic Range - Nikon Eclipse 50i Instructions Manual

Image Capture

3.18
Images can be captured by mounting a camera head to the ergonomic binocular tube or
trinocular eyepiece tube.
For more detailed discussion of this topic, refer to the operating manual provided with the
camera head or camera control software.
Proper adjustment of light intensity and focus on the microscope side are important for obtaining
clear images. Listed below are key considerations in capturing clear images.

Adjusting light intensity

3.18.1
Lamp voltage: When the 50i is used in applications for which accurate color reproduction is
Filter:

Adjusting the condenser

3.18.2
Always focus and center the condenser.
•
Center the annular diaphragm for phase contrast microscopy.
•
For normal operations, set the diaphragm aperture to 70 to 80% of the N.A. of the
•
objective.

Confirming the photomicrographic range

3.18.3
The image on the monitor represents the photomicrographic range.

Confirming focus

3.18.4
Check focus by viewing through the eyepiece and viewing the monitor. If the focal positions for
the two images differ, adjust the focal position adjustment screw at the camera port.
Chapter 3 Individual Operations
3.18 Image Capture
critical, set the brightness control knob at a midpoint and use ND filters to make
brightness adjustments.
Place a commercially available color compensation filter on the filter holder at the
microscope base, as necessary.
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