Water Immersion - Nikon Eclipse 50i Instructions Manual

Chapter 3 Individual Operations

3.14 Water Immersion

Water Immersion
3.14
Objectives marked "WI" or "W" are water-immersion objectives. These objectives are used with
immersion water (distilled water or physiological saline) applied between the specimen and the
tip of the objective. Microscopy procedures are the same as for oil-immersion objectives.
Since water evaporates readily, monitor the immersion water during observation. Avoid using
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too much water, since excess water will flow onto the stage and around the condenser,
promoting corrosion.
After use, wipe off water from the tip of the objective and condenser, then follow up by wiping
with absolute alcohol.
If you observe water stains, apply a small amount of neutral detergent and wipe gently, then
follow up with absolute alcohol.
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