Photomicroscopy - Nikon Eclipse 50i Instructions Manual

Photomicroscopy

2.4
For detailed discussions of the camera, photomicroscopic software, and PC, refer to the
operating manuals provided with the respective products. The following instructions assume a
DS-5M digital camera and DS-L1 camera control unit.
1
Adjust the microscope for proper image observation.
See the directions given in sections from "1. Bright-Field Microscopy" to "3. Epi-fluorescence
Microscopy."
2
Adjust the camera head mounting position until the image is
displayed properly.
(1) Adjustment based on stage motion
direction
Loosen the C mount installation direction
locking screw and adjust the camera position
so that moving the stage forward-backward
or right-left moves the image on the monitor
in opposite directions. After making the
appropriate adjustments, tighten the screw to
secure the C mount into place.
(2) Focus adjustment
If the image viewed through the eyepiece
appears to be in focus but the image on the
monitor is out of focus, turn the camera fine
focus adjustment ring on the C mount until
the image on the monitor is in focus.
Note that such out of focus situations can also
indicate incorrect diopter adjustment. Make
sure you have made diopter adjustments.
(P.43)
(3) Centering the camera
Turn the right and left camera centering
screws to align the image seen through the
eyepiece with the image on the monitor.
3
Make camera settings.
For detailed discussion, refer to the operating manual provided with the camera.
When using the DS-L1, you must choose and enter at least the following information:
• Folder for data storage.
• Name of file to be saved. (You can select "Auto.")
• File format and file size.
• Date and destination of data
Chapter 2 Microscopy
2.4 Photomicroscopy
32
(A)
Adjusting (A) and (B)
Camera centering screws
Installation direction
locking screw
(B)
Camera
fine focus
adjustment ring