Microscopy With Epi-Fl Attachment Mounted - Nikon ECLIPSE 50i Instructions Manual

Microscopy with Epi-fl Attachment Mounted

2.3
Before microscopy
• Check the cumulative operating hours of the mercury lamp. Replace the lamp if
its cumulative operating hours exceed the average service life.
• Use non-fluorescent slide glass.
• Use non-fluorescent immersion oil.
• To keep the specimen color from fading, keep the shutter closed when not
performing microscopy.
1
Perform steps 1 through 10 in "2.1 Bright-Field Microscopy."
2
Turn off the microscope power
switch.
3
Close the shutter and block the
light emitted by the mercury lamp.
⇒ P.51
4
Insert the desired excitation filter
cube into the optical path.
⇒ P.52
Chapter 2 Microscopy
2.3 Microscopy with Epi-fl Attachment Mounted
29
Press the power switch
to the "◯" position.
Close
the shutter.
Select a cube using the filter
cube switching knob.
2