Princeton Instruments Activity Tracker User Manual page 44

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2. Adjust the lens aperture, intensity scaling, and focus for the best image as viewed on
the monitor. Some imaging tips follow.
a. Begin with the lens blocked off. Set the lens at the smallest possible aperture
b. Place a suitable target in front of the lens. An object with text or graphics works
c. Adjust the intensity scaling and lens aperture until a suitable setting is found.
d. Set the focus adjustment of the lens for maximum sharpness in the viewed
e. If the camera is mounted to a microscope, first be sure to have a clear, focused
f. In the case of a camera with an F-mount, the adapter itself also has a focus
MicroMAX System User Manual
Note: If you are using WinView/32 (or WinSpec/32 in Imaging Mode) and a
video monitor for focusing, select the
Acquisition menu. Then select a short exposure time (0.1 s), an Intensity
Scaling setting of 4096, and 2x Zoom. With an MicroMAX:1300Y camera
(1030×1300 pixels), set the Pan selector as required for the 756×486 subset of the
array image you wish to use for focusing purposes. Select the center pan position
if the camera is a MicroMAX:782Y (782×582 pixels) or a MicroMAX:512BFT
(512×512 pixels). Begin data collection by selecting RUN on the Interactive
Camera Operation dialog box. The shutter, if present, will open and successive
images will be sent to the monitor as quickly as they are acquired, giving as close
to continuous video as possible.
(largest f-stop number).
best. If working with a microscope, use any easily viewed specimen. It is
generally not advisable to attempt fluorescence imaging during this Getting
Started phase of operation.
The initial intensity scaling setting of 4096 assures that the image won't be
missed altogether but could be dim. Once you've determined that the image is
present, select a lower setting for better contrast. Check the brightest regions of
the image to determine if the A/D converter is at full-scale. The A/D converter is
at full-scale when any part of the image is as bright as it can be. Adjust the
aperture to where it is just slightly smaller (higher f stop) than the setting where
maximum brightness on any part of the image occurs.
image.
image through the eyepiece. Then divert the light to the camera and lower the
illuminating light intensity.
To adjust the parfocality on an F-mount system, begin collecting images
o
with a short exposure time and focus the light on the camera by rotating the
ring on the Diagnostic Instruments relay lens without touching the main
focusing knobs on the microscope.
o In the case of a camera with an F-mount lens adapter, focusing is normally
done by means a focus adjustment on the relay-lens adapter.
o On a C-mount system, the camera should be very close to parfocal, although
some C-mounts will be adjustable using setscrews on the microscope to
secure the adapter slightly higher or lower in position.
adjustment. If necessary, this focus can be changed to bring the image into range
of the lens focus adjustment. The lens-mount adjustment is secured by four
mode from the
Video Focus...
Version 6.B