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Summary of Contents for Leica TCS SP5
- Page 1 Leica TCS SP5 Leica TCS SP5 X User Manual...
- Page 2 Published by: Leica Microsystems CMS GmbH Am Friedensplatz 3 D-68165 Mannheim (Germany) http://www.leica-microsystems.com http://www.confocal-microscopy.com Responsible for contents: Leica Microsystems CMS GmbH Copyright © Leica Microsystems CMS GmbH. All rights reserved.
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Page 3: Table Of Contents
Overview of Usable MP Lasers (IR Lasers) ............20 2.8.2.1 Picosecond laser ..................20 2.8.2.2 Femtosecond laser ..................21 2.8.3 Overview of Usable VIS/UV Lasers for TCS SP5 X........22 2.8.4 Which Laser Class Does the Product Have? ..........23 2.8.5 Required Laser Safety Measures..............23 Safety Instructions and their Meanings............25... - Page 4 General Safety Instructions ................27 Laser Class for VIS and UV Systems............... 27 Laser Class for MP Systems ................27 What does the owner/operator have to observe? ..........27 Safety Instructions for the User ................ 29 Operational Reliability ..................29 Maximum Current Load of the Multiple Socket Outlet at the Supply Unit ..
- Page 5 6.3.3 VIS and UV Systems with Inverted or Upright Microscope ......54 Specimen Area ....................55 Changing Specimens..................56 Changing Objectives..................57 Changing the Transmitted-Light Lamp Housing..........58 Mirror housing on upright microscope...............60 Changing Filter Cubes, Beam Splitters or Condenser ........62 6.10 Piezo focus on upright microscope ..............63 6.10.1 Objective Change with Piezo Focus Configuration .........64 Starting Up the System ..................65...
- Page 6 10.2.5 The Pinhole and Its Effects................93 10.2.6 Image Detail and Raster Settings..............95 10.2.7 Signal and Noise .................... 99 10.2.8 Profile Cuts ....................101 10.3 Multiparameter Fluorescence................. 102 10.3.1 Illumination ....................102 10.3.2 Beam Splitting ....................104 10.3.3 Emission Bands.................... 104 10.3.4 Crosstalk ......................
- Page 7 11.3 Cleaning the Microscope Surface ..............116 11.4 Maintaining the Scanner Cooling System ............116 Transport and Disposal...................117 12.1 Changing the Installation Location..............117 12.2 Disposal ......................117 Contact ......................117 Glossary ......................118 Appendix ......................124 15.1 Safety Data Sheets from Third-party Manufacturers........124 15.2 Declaration of conformity ................129 15.3 People´s Republic of China ................130...
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Page 9: General
The information contained in this operating manual is subject to change without prior notice. All rights to this documentation are held by Leica Microsystems CMS GmbH. Adaptation, translation and reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. -
Page 10: About This Operating Manual
The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the Leica TCS SP5 and Leica TCS SP5 X. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. -
Page 11: Leica Tcs Sp5 / Tcs Sp5 X
2. Leica TCS SP5 / TCS SP5 X TCS SP5 system overview Figure 1: TCS SP5 system components (overview) TCS SP5 Scanner Main switch board TCS workstation Supply unit Control panel Microscope... -
Page 12: Tcs Sp5 X System Overview
TCS SP5 X system overview Figure 2: TCS SP5 X system components (overview) TCS SP5 X scanner Main switch board TCS workstation Supply unit Control panel Microscope Achromatic light laser Intended Use The system was designed for confocal scanning (laser scanning images) of fluorescence- marked living and fixed specimens as well as for quantitative measurements in the area of life science. -
Page 13: Conformity
The manufacturer assumes no liability for damage caused by, or any risks arising from, use of the microscopes for purposes other than those for which they are intended, or not using the microscopes within the specifications of Leica Microsystems CMS GmbH. In such cases, the Declaration of Conformity shall be invalid. -
Page 14: Patents
Patents The Leica TCS SP5 product is protected by the US patents: 5,886,784; 5,903,688; 6,137,627; 6,222,961; 6,285,019; 6,311,574; 6,355,919; 6,423,960; 6,433,814; 6,444,971; 6,466,381; 6,510,001; 6,614,526; 6,654,165; 6,657,187; 6,678,443; 6,687,035; 6,738,190; 6,754,003; 6,801,359; 6,831,780; 6,850,358; 6,867,899. Further patents are pending. The Leica TCS SP5 X protect by the US patents: 5,886,784;... -
Page 15: Specifications
2.7.1.1 TCS SP5 with inverted microscope 1400 mm 730 mm 600 mm 2540 mm 1200 mm Figure 4: Dimensions of TCS SP5 with inverted microscope 2.7.1.2 TCS SP5 with upright microscope 1490 mm 730 mm 600 mm 2400 mm 1200 mm... -
Page 16: Tcs Sp5 X With Inverted Microscope
2.7.1.3 TCS SP5 X with inverted microscope Figure 6: Dimensions of TCS SP5 X with inverted microscope 2.7.1.4 TCS SP5 X with upright microscope Figure 7: Dimensions of TCS SP5 X with upright microscope... -
Page 17: Electrical Connection Requirements
2.7.2.2 Electrical connection requirements of achromatic light laser Supply voltage: 100 - 240 V AC ± 10 % Frequency: 50/60 Hz Power consumption: 2.2 A Overvoltage category: Fuse 2x T4A, 250V AC Applies only to the TCS SP5 X system. -
Page 18: Requirements Regarding Ambient Conditions
2.7.3 Requirements Regarding Ambient Conditions Do not expose the system to drafts. Ensure that the system is not installed next to air conditioners or ventilation systems. For this reason, the installation location should be carefully planned. Ensure that the environment is as dust-free as possible. Also read the notes on protection against dust in Chapter 11 Care and Maintenance Installing the system in darkened rooms is also advisable. -
Page 19: Waste Heat/Required Cooling Performance
2.7.5 Waste Heat/Required Cooling Performance The TCS SP5 system has a maximum power consumption of 3.2 kW (VIS system) or 6.2 kW (MP system). The TCS SP5 X has a maximum power consumption of 3.4 kW. For the specifications of external lasers such as UV and MP lasers, please refer to the manufacturer's documentation. -
Page 20: Overview Of Usable Mp Lasers (Ir Lasers)
690 - 1040 < 4 < 1.9 pulsed, 1.0 - 1.5 ps Ultra I Chameleon ps 680 - 1080 < 4 < 1.9 pulsed, 1.0 - 1.5 ps Ultra II Table 2: Usable MP lasers for TCS SP5 and TCS SP5 X... -
Page 21: Femtosecond Laser
140 fs Chameleon Compact OPO 1000 - 1600 < 1.6 < 0.8 pulsed > 100 fs Table 3: Usable MP lasers for TCS SP5 and TCS SP5 X The components for laser safety are designed only for the laser variants listed. -
Page 22: Overview Of Usable Vis/Uv Lasers For Tcs Sp5 X
2.8.3 Overview of Usable VIS/UV Lasers for TCS SP5 X The Leica TCS SP5 X features a combination of the lasers listed below. Maximum Maximum luminous power luminous power Wavelength at laser output in focal plane Laser type [nm] Pulse duration... -
Page 23: Which Laser Class Does The Product Have
2.8.4 Which Laser Class Does the Product Have? Laser Wavelength range Configuration Laser class variant Combination of lasers from Chapter 2.8.3 or 2.8.1 400 - 700 nm, (without lasers having 3B / IIIb (visible laser radiation) wavelengths of 350 - 400 nm) 350 - 700 nm, Combination of lasers (visible and invisible... -
Page 25: Safety Instructions And Their Meanings
3. Safety Instructions and their Meanings DANGER This kind of warning alerts you of an operating procedure, practice, condition, or instruction in the operating manual that must be strictly observed and followed, as otherwise you expose yourself to the risk of fatal injury. WARNING! LASER RADIATION A laser warning points out an operation, a process, a condition or an instruction that must be observed strictly to prevent serious eye injuries to the persons using... - Page 26 Notes either contain additional information on a specific topic or special instructions on the handling of the product.
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Page 27: General Safety Instructions
4. General Safety Instructions Laser Class for VIS and UV Systems In accordance with IEC/EN 60825-1, this system is a laser product of Class 3B / IIIb. Never expose eyes or skin to direct radiation! The laser light can cause permanent eye damage! Laser Class for MP Systems In accordance with IEC/EN 60825-1, this system is a laser product of Class 4 / IV. - Page 28 Leica service representatives. If repairs or service measures are performed that require opening parts of the housing, only trained Leica service technicians may occupy the room in which the system is located. Do not connect any external equipment or other components.
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Page 29: Safety Instructions For The User
Safety Instructions for the User Read and observe the safety instructions in the operating manual and the safety labels located on the system. Failure to observe the safety instructions may lead to serious injuries and to significant damages to the system and loss of data. The instrument is a Class 3B or 4 laser product (depending on the laser used). -
Page 30: Maximum Current Load Of The Multiple Socket Outlet At The Supply Unit
Maximum Current Load of the Multiple Socket Outlet at the Supply Unit The total power consumption of all loads connected to the multiple socket outlet (Figure 8) must not exceed 800 VA. The terminals are intended for: • TCS workstation •... -
Page 31: Safety Devices
5. Safety Devices Disconnecting the Power Supply The main circuit breaker is located on the right rear side of the supply unit. It is used to de- energize the complete system using a single switch (Figure 9). The main circuit breaker functions as a switch and as an overcurrent fuse. The main circuit breaker is not to be used as the regular on/off switch for the system. -
Page 32: Detachable-Key Switch
Detachable-key Switch The detachable-key switch for protection against unauthorized use of the laser products is located on the main switch board (see Figure 10). Figure 10: Detachable-key switch for the internal lasers The detachable-key switch for protection against unauthorized use of the external achromatic light laser is located on the front of the achromatic light laser (see Figure 11). - Page 33 The key switch for protection against unauthorized use of the external UV laser is located on the front of the power supply (see Figure 12). Figure 12: Key switch for the external UV laser For other external lasers, please refer to the operating manual supplied by the laser manufacturer for the position of the detachable-key switches.
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Page 34: Emissions Warning Indicators
Emissions Warning Indicators The operational readiness of lasers located in the supply unit is signaled by an emission warning indicator (Figure 13). The emission warning indicator is located above the detachable-key switch and is yellow when lit. The emission warning indicator of the achromatic light laser is located on the front of the achromatic light laser (see Figure 14) and is red when lit. -
Page 35: Remote Interlock Connection On The Supply Unit
Scanning of the specimen is not activated after being switched on properly (laser radiation in the specimen area). Contact Leica Service immediately. For other external lasers, please refer to the operating manual supplied by the laser manufacturer for the position of the emission warning indicator. -
Page 36: Remote Interlock Connection On The Achromatic Light Laser
The laser beam path is interrupted if the contact is open. Figure 17: Remote interlock connection at the achromatic light laser Applies only to the TCS SP5 X system. -
Page 37: Remote Interlock Connections On External Lasers
Remote Interlock Connections on External Lasers For external lasers, please refer to the operating manual supplied by the laser manufacturer for the position of the remote interlock connection. Remote interlock jack/interlock connector on the scanner The interlock jack is located on the rear side of the scanner (operating voltage: 12 V DC, Figure 18). -
Page 38: Function And Position Of Safety Switches
Function and Position of Safety Switches When the safety switches are released, the light path of the laser beam is interrupted. Figure 19: Position of the transmitted-light illumination arm (1) and switching from scan mode to eyepiece (2). Type of Position Activated by: Activated if: Function... -
Page 39: Special Laser Safety Equipment
Special Laser Safety Equipment 5.9.1 Laser protection tube and beam stop On inverted microscopes, the safety beam guide and the beam stop serve as protection against laser radiation emission and are located between the condenser base and the transmitted-light detector (see Figure 20). Laser protection tube Beam stop (illustrated is the version of the beam... -
Page 40: Shielding In Mp Systems (Ir Lasers)
5.9.2 Shielding in MP Systems (IR Lasers) The light of all employed VIS lasers (wavelength range 400 - 700 nm, visible spectrum) and UV lasers (wavelength range < 400 nm, invisible) is fed through a fiber optic cable and, therefore, completely shielded until it leaves the microscope objective and reaches the specimen. -
Page 41: Safety Labels On The System
5.10 Safety labels on the system The corresponding safety labels are selected dependent on the laser configuration (VIS, UV, MP) and attached in the following locations either in the English or German language. 5.10.1 Inverted microscope DMI 6000 CS Angled rear view of right side of microscope: Figure 22: Safety label for inverted microscope DMI 6000 CS... - Page 42 Angled front view of right side of microscope: Figure 23: Safety label for inverted microscope DMI 6000 CS...
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Page 43: Upright Microscope Dm 5000/6000 Cs
5.10.2 Upright microscope DM 5000/6000 CS Angled front view of right side of microscope: Figure 24: Safety label for upright microscope DM 5000/6000 CS... - Page 44 Rear view of microscope: Figure 25: Safety label for upright microscope DM 5000/6000 CS...
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Page 45: Scan Head
5.10.3 Scan Head Angled front view of left side of scan head: Figure 26: Safety label for the scanner... -
Page 46: Achromatic Light Laser
5.10.4 Achromatic light laser Rear side of achromatic light laser: Figure 27: Safety label on the rear side of the white light laser Applies only to the TCS SP5 X system. -
Page 47: External Uv Laser
5.10.5 External UV laser Figure 28: Safety label on external UV laser Applies only to systems with an external UV laser. -
Page 48: Supply Unit
5.10.6 Supply Unit View of supply unit: Figure 29: Safety label for the TCS SP 5 supply unit (front side) -
Page 49: Mp Beam Coupling Unit
5.10.7 MP beam coupling unit Angled front view of the right side of the MP beam coupling unit: Figure 30: Safety label for the MP beam coupling unit (top side) -
Page 50: Cover (For Replacement Flange)
5.10.8 Cover (for Replacement Flange) Front view of cover: Figure 31: Cover for replacement flange If the replacement flange for transmitted light is not equipped with a functional module such as a lamp housing, a cover must be placed over the opening for laser safety reasons.
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