Leica TCS SP2 Brief Manual
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Leica TCS SP2 Brief Manual

Leica TCS SP2 Brief Manual

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Brief manual how to start and close the Leica sp2 Confocal
(TCS SP2 AOBS system mounted on a DM IRE2)

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Summary of Contents for Leica TCS SP2

  • Page 1 Brief manual how to start and close the Leica sp2 Confocal (TCS SP2 AOBS system mounted on a DM IRE2)
  • Page 2 A. Switching on hardware B. Acquiring and saving images C. Switching off the microscope D. Good working practice Assistance/instructions/any problem: Liesbeth Pierson, 024-3652012, Room 01.239, GI. e.pierson@science.ru.nl Black = obligatory Gray = optional settings A. Switching on hardware  Switch red knob #1 on and press on the PC ON button to start Windows (the monitor is switched on;...
  • Page 3  Choose the correct sample holder (slide holder for xy scans, or slide holder with outstaying box for xz scans, or multiwells holder).  To start the Leica confocal software that commands the hardware (microscope, lasers, AOBS and more) and contains the acquisition and imaging modules, click on the Leica LCS icon on the screen.
  • Page 4  To view the specimen in bright field (BF), 4 things to do: turn the power wheel # 11 on to about 4V (for white light at 3200 K around 8V, to be used with NDF) and the lollypop shutter above the field diaphragm in down position, Knob #12 operating a mirror in the condenser light path should be on VIS = visual and MicCtrl on Visual.
  • Page 5 B. Acquiring and saving images  In the Leica Confocal Software (icon on desktop): the "Acquire" menu appears on the left monitor. Images will be displayed on the right monitor.  Switch on appropriate lasers and push the control for each wavelength to the desired level in %.
  • Page 6  Objectives can be automatically selected by clicking the from the “Obj.“ menu in the lower left corner.  Choose a “Menu” for the acquisition from the Leica or User Window. For the xy sample holder choose z-scan icon> z-wide. For the xz sample holder, choose z-scan icon>...
  • Page 7 C. Switching off the microscope  Wipe immersion medium (oil, glycerol) from the 63x objectives using a fiber-free tissue (small rectangles) for oil and glycerol, or a cleenex tissue to absorb water at the 40x objective .  Set the 10x objective in the upright position ...

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