Related Manuals for Levenhuk MAGUS LUM 400L
Summary of Contents for Levenhuk MAGUS LUM 400L
- Page 1 M AG US LUM 4 00 L F LU OR ESC EN C E D I G I TA L MI C RO S C O P E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2025 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20250321...
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Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 4 11. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 12. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired.
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Page 5: Table Of Contents
CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 MICROSCOPE PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage Reflected light illuminator 3 UNPACKING AND ASSEMBLING THE MICROSCOPE 4 BRIGHTFIELD OBSERVATIONS IN TRANSMITTED LIGHT Switching on the illumination Placing the specimen Focusing on the specimen Adjusting the eyepiece tubes... -
Page 6: Description Of The Microscope
MAGUS Lum 400L Fluorescence Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. -
Page 7: Microscope Kit
Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing lock knob Coarse focusing tension adjusting knob Transmitted light source 3W LED Reflected light source Four 5W LEDs of different wavelengths AC voltage 85–265V, 50/60Hz Power supply... - Page 8 Fig. 1. MAGUS Lum 400L Fluorescence Microscope. View from the right 1. Trinocular tube 9. Transmitted and reflected light 15. Filter slider (shutter/neutral brightness adjustment ring density filter/free slot) 2. Eyepieces 10. Coarse focusing tension 16. Field aperture centering knob of 3.
- Page 9 Fig. 2. MAGUS Lum 400L Fluorescence Microscope. View from the left 1. Diopter adjustment ring 7. Transmitted-light field 12. Coarse focusing knob diaphragm ring 2. Revolving nosepiece 13. Coarse focusing lock knob 8. Stand and base 14. Condenser focus knob 3.
- Page 10 Fig. 3. MAGUS Lum 400L Fluorescence Microscope. Rear view 1. Transmitted and reflected light illuminator switch 3. ON/OFF switch 2. Connector for the power cord of the reflected 4. Connector for the microscope power cord light illuminator...
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Page 11: Microscope Parts
2 MICROSCOPE PARTS STAND The stand 8 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 2 (Fig. 2) with objectives 3 (Fig. 2) – stage 4 (Fig. 2) –... -
Page 12: Eyepieces
The interpupillary distance is adjusted by rotating the eyepiece tubes 3 (Fig. 1) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale. For convenience, the microscope head is inclined at 30°. Microscope head magnification: 1x. -
Page 13: Condenser
The specifications of the objectives (Table 2): Numerical Working Coverslip, Color Objective identification System Magnification aperture distance, mm marking PL 4х/0.10 ∞/– 4х 0.10 19.8 – PL 10х/0.25 ∞/– 10х 0.25 – yellow PL FL 10х/0.35 ∞/– 10х 0.35 – yellow PL 20х/0.40 ∞/0.17 20х... -
Page 14: Reflected Light Illuminator
REF LECTE D LIGH T IL LU MI NATO R The general view of the reflected light illuminator is given in Fig. 4. Fig. 4. Reflected light illuminator 1. Illuminator body 5. Filter slider 8. Lamphouse locking screw 2. Turret wheel 6. -
Page 15: Unpacking And Assembling The Microscope
The excitation spectrum range: 320–550nm. The emission spectrum range: 435–700nm. The specifications of the reflected light illuminator (Table 3): Filter type LED excitation wavelength, nm Dichroic mirror wavelength, nm Emission wavelength, nm Ultraviolet (UV) 320–380 Violet (V) 380–415 Blue (B) 410–490 Green (G) 475–550... -
Page 16: Brightfield Observations In Transmitted Light
5. Loosen the attachment screw 2 using an Allen wrench. Remove the microscope head 3 from the stand. 6. Remove the reflected light illuminator 5 and the lamphouse with LEDs 6 from their packages. Remove dust covers from the reflected light illuminator. 7. -
Page 17: Placing The Specimen
PL AC IN G THE SP EC IM E N Place the specimen 1 on the stage. Adjust the image by moving the stage control knobs 2 and 3 so that the observed section of the specimen is directly under the objective. The stage attachment features an XY control system. -
Page 18: Adjusting The Eyepiece Tubes
ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale "0" to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus. -
Page 19: Setting Up Köhler Illumination
SE TTI N G U P KÖ H L ER IL LU MI NAT IO N In the light optical microscope, the image quality depends equally on the optics and on the illumination system, so adjusting the illumination is an important preparatory step. The illumination system affects the image resolution, comfort during long observation, and photo quality when using digital cameras. -
Page 20: Using Oil Immersion Objectives
When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field. -
Page 21: Fluorescence Observations
5 FLUORESCENCE OBSERVATIONS Before starting fluorescence observations, adjust the microscope to work in transmitted light using the brightfield technique, as described above. Placing the specimen, focusing on the specimen and adjusting the microscope head for the fluorescence observations are done in the same way as for the transmitted light observations. During fluorescence observations: –... - Page 22 2. Selecting the fluorescence filter The rotating turret accommodates four fluorescence filters: blue (B), green (G), violet (V), and ultraviolet (UV). Rotate the ring 4 to select one of the filters in accordance with the used fluorochrome. The marking of each filter corresponds to the color of the excitation light and is inscribed on the wheel as a symbol.
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Page 23: Using Optional Equipment
– If the bright spot is not in the center of the field of view, as shown in Fig. 13а, you will need to center the diaphragm. Use two centering screws 1 located on both sides of the illuminator to align the center of the diaphragm with the center of the field of view, as shown in Fig. -
Page 24: Darkfield Slider
DARKF I EL D SL ID ER The darkfield slider is designed for the darkfield microscopy on objectives with apertures up to 0.9. The slider is a plate with two round openings. One opening is free for the brightfield technique. The second opening holds the darkfield diaphragm. The slider is inserted in the slot of the Abbe condenser 10 (Fig. -
Page 25: Using The Camera
Work out the value for one eyepiece division using each objective by formula Е = ТL/A, where: E – eyepiece division value Т – stage division value specified on the stage micrometer (0.01mm) L – number of stage micrometer divisions A –... -
Page 26: Using The Calibration Slide With A Camera
– Pull out the beam splitter lever 4 as far as it will go. The knob is in the pushed-in position when the trinocular tube is not used. – Switch on the camera according to the manual, adjust the image. –... -
Page 27: Troubleshooting
7 TROUBLESHOOTING Potential problems and remedies (Table 4): Problem Cause Remedy ELECTRI CAL COMP ONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in the field of view The circuit board connector has poor Have the connector repaired by a qualified contact electronics technician... -
Page 28: Mechanical Components
MECHANI CAL C OMPONENTS The image does not remain The coarse focusing tension adjusting Adjust the coarse focusing tension adjusting sharp during observation knob is loosened, causing the stage knob to lower spontaneously The coarse focusing knob The coarse tension adjusting knob Loosen the tension of the coarse is too tight to rotate is overtightened... -
Page 29: Care And Maintenance
UV protective glass (shield) Filter slider In the reflected light illuminator Black protective plate for under-stage installation C-mount camera adapter Digital camera Optional Monitor Optional Calibration slide Optional ACCESSORIES AND SPARE PARTS Allen head screw for the reflected light illuminator Installed in the stand socket Head locking screw Installed in the illuminator socket... -
Page 30: Maintenance
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 32 www.magusmicro.com...
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