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Summary of Contents for Levenhuk MAGUS LUM V500L
- Page 1 M AG US LUM V 5 0 0 L I NV ERT ED F LUO R ES CENC E MI CRO S C O P E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241031...
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Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. -
Page 4: Table Of Contents
CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 MICROSCOPE PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Transmitted light illuminator Stage Reflected light illuminator 3 UNPACKING AND ASSEMBLING THE MICROSCOPE 4 BRIGHTFIELD OBSERVATION PROCEDURE Switching on the illumination Using the beam splitter lever Adjusting the height and position of the condenser Adjusting the eyepiece tubes... -
Page 5: Description Of The Microscope
MAGUS Lum V500L Inverted Fluorescence Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. - Page 6 Optical design Infinity plan achromatic, parfocal distance: 45mm; long focal length Objectives PLL 10х/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5 (spring-loaded); magnification, x/aperture/ phase-contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; working distance, mm PLL 40x/0.60/3.5 PHP2 (spring-loaded); PL 4x/0.10* Fixed stage Stage size: 227mm × 208mm Glass stage plate with a diameter of 118mm Mechanical XY stage attachment Moving range: 77mm×134.5mm...
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Page 7: Microscope Kit
M ICROS COPE K IT The microscope kit includes the following main components: – stand with a built-in power supply, transmitted light source, fluorescence filter assemblies, focusing mechanism, fixed stage, revolving nosepiece, condenser mount, and trinocular tube. – trinocular head –... - Page 8 Fig. 1. MAGUS Lum V500L Fluorescence Microscope. View from the left 1. Eyecups 10. Fluorescence filter assembly 2. Eyepieces 11. Revolving nosepiece 3. Stand 12. Objectives 4. Transmitted/reflected light illuminator switch 13. Stage 5. ON/OFF switch 14. Condenser mount 15. Transmitted light illuminator 6.
- Page 9 Fig. 2. MAGUS Lum V500L Fluorescence Microscope. View from the right 1. Interpupillary distance adjustment ring 10. Reflected light illuminator cable 2. Eyepiece tubes 11. Object moving knob 3. Knob for switching the light path to the trinocular tube 12. Dish holder 4.
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Page 10: Microscope Parts
MICROSCOPE PARTS STAND The stand has stable ergonomic design. Parts attached to the microscope stand 3 (Fig. 1): – revolving nosepiece 11 (Fig. 1) with objectives – stage 13 (Fig. 1) – condenser mount 14 (Fig. 1) – transmitted light illuminator 15 (Fig. 1) –... -
Page 11: Microscope Head
MIC ROS COPE H E AD The trinocular head provides the visual observation of the specimen image. The microscope head is pre-installed on the stand 3 (Fig. 1). The eyepiece tube assembly is inserted into the head socket and secured with a screw. When installing the eyepiece tubes 2 (Fig. -
Page 12: Transmitted Light Illuminator
The 40x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object. If objectives are damaged, we recommend repairing them in the service center. The objectives are intended to image the specimens through air. Do not use immersion oil. T RANS M ITTED LI GHT I LLUM INATO R The microscope illuminator allows for setting up Köhler illumination. -
Page 13: Unpacking And Assembling The Microscope
The microscope employs narrow-band color filters. The excitation occurs in a selected wavelength range, and the emission filter allows the narrow spectrum of the specific wavelength to pass through. For example, the "DAPI" position means that the fluorescence filter introduced in the optical path emits monochromatic radiation of the ultraviolet spectrum range of 348-382nm. -
Page 14: Brightfield Observation Procedure
3. Take out the eyepiece tube assembly 2, remove the dust cover. Insert the assembly into the socket 3. Move the eyepiece tubes upwards or downwards to adjust them to the observer’s height. Secure the eyepiece tube assembly with a screw using an Allen wrench from the microscope kit. 4. -
Page 15: Using The Beam Splitter Lever
Turn the ON/OFF switch 3 to "–" position. Set switch 2 to the "II" position, which corresponds to the transmitted light observations. Adjust the brightness using the ring 1 so that the light brightness is comfortable for your eyes. Do not keep the brightness adjustment ring in the maximum brightness position for a long period. -
Page 16: Placing The Specimen
P LACI NG THE S P EC IME N Choose the appropriate dish holder from the kit based on its shape and size (Fig. 10). Install the dish holder on the stage. Fix the slide 1 or the dish with the studied sample in it. Fig. -
Page 17: Setting Up Köhler Illumination
When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob. Adjust the coarse focusing tension. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use. -
Page 18: Observing Specimens In Tall Glassware
O BSE RVI N G SP EC IME N S IN TAL L GLAS SWA R E The microscope is equipped with a stage attachment 1 that enables movement of the specimen in longitudinal (Y) and lateral (X) directions. The object is moved by Y-axis 2 and X-axis 3 coaxial knobs, as shown in Fig. -
Page 19: Calculating The Field Of View
CALCUL ATIN G T H E FI E LD O F VI E W The field of view is calculated by dividing the eyepiece field number by the objective magnification. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the field of view of the microscope is 22mm/40х... -
Page 20: Fluorescence Observations
When using other phase-contrast objectives, place the corresponding phase-contrast annulus into the optical path. 2. Mount the specimen on the stage, focus the microscope. 3. Remove the eyepiece from the tube and insert the centering telescope 2 instead. You will see a dark ring 5 and a light ring 6 in the field of view. -
Page 21: Using Optional Equipment
USING OPTIONAL EQUIPMENT USING THE CAME RA The microscope is designed to observe a specimen through the eyepieces and to photograph the specimen. The microscope has two ports: on the microscope head and on the stand. The monitor is conveniently mounted in the trinocular tube of the microscope head, and the digital camera –... -
Page 22: Using The Eyepiece With A Scale
Do it as follows: – Set the beam splitter lever 3 to the eyepiece/camera position. While observing the specimen through the eyepieces, find a distinctive point in the field of view (an easily identifiable target, such as point S in Fig. 19а), move the specimen on the stage so that the point is in the center of the field of view, as shown in Fig. -
Page 23: Using The Calibration Slide With A Camera
U SING THE CAL I B R ATI O N SLI D E WI T H A CA M ER A The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification and indicate the known distance. - Page 24 The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate dish bottom thickness Use the glassware with a standard bottom thickness (1.2mm) Poor image quality (low resolution, poor contrast) The aperture diaphragm is opened too Adjust the opening to match the numerical wide aperture of the objective used...
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Page 25: Scope Of Delivery
SCOPE OF DELIVERY The scope of delivery (Table 5) Component Note MAIN COMPONENTS Stand with a built-in power supply, transmitted light source, focusing mechanism, condenser mount, and trinocular tube Fluorescence filter assembly Mounted in the stand Trinocular microscope head Revolving nosepiece Mounted on the stand Fixed stage Mounted on the stand... -
Page 26: 10 Care And Maintenance
10 CARE AND MAINTENANCE R E PL AC IN G T HE BULB This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired. -
Page 27: Magus Warranty
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 28 www.magusmicro.com...
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