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Summary of Contents for Levenhuk MAGUS LUM D400L
- Page 1 M AG US LUM D4 00 L FLU OR ESC E NC E D I G ITA L MI C RO S C OP E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241105...
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Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 4 11. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 12. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired.
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Page 5: Table Of Contents
CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 MICROSCOPE PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage Reflected light illuminator Camera 3 UNPACKING AND ASSEMBLING THE MICROSCOPE 4 BRIGHTFIELD OBSERVATIONS IN TRANSMITTED LIGHT Switching on the illumination Placing the specimen Focusing on the specimen... -
Page 6: Description Of The Microscope
MAGUS Lum D400L Fluorescence Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. - Page 7 Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing lock knob Coarse focusing tension adjusting knob Transmitted light source 3W LED Reflected light source Four 5W LEDs of different wavelengths AC voltage 85–265V, 50/60Hz Power supply...
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Page 8: Microscope Kit
Software MAGUS View Mount type C-mount Body material metal Power supply DC 5V from the USB port of the computer Dimensions without package (WxHxD) 237mm×502mm×427mm Package dimensions (WxHxD) 302mm×780mm×452mm Weight 12.3kg Weight with package 14.8kg * Not included in the kit, available on request. ** The magnification of the microscope can be increased by using additional (optional) eyepieces and objectives The manufacturer reserves the right to make changes to the product range and specifications without prior notice. - Page 9 Fig. 1. MAGUS Lum D400L Fluorescence Microscope. View from the right 1. Trinocular tube 9. Transmitted light brightness 15. Filter slider (shutter/neutral adjustment ring density filter/free slot) 2. Eyepieces 10. Coarse focusing tension 16. Field aperture centering knob of 3. Eyepiece tubes...
- Page 10 Fig. 2. MAGUS Lum D400L Fluorescence Microscope. View from the left 1. Diopter adjustment ring 8. Stand and base 15. Field diaphragm centering knob 2. Revolving nosepiece 9. Condenser locking screw 16. LED switching ring 17. Beam splitter lever 3. Objective 10.
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Page 11: Microscope Parts
2 MICROSCOPE PARTS STAND The stand 8 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 2 (Fig. 2) with objectives 3 (Fig. 2) – stage 4 (Fig. 2) –... -
Page 12: Eyepieces
The interpupillary distance is adjusted by rotating the eyepiece tubes 3 (Fig. 1) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale. For convenience, the microscope head is inclined at 30°. Microscope head magnification: 1x. -
Page 13: Condenser
The specifications of the objectives (Table 2): Numerical Working Coverslip, Color Objective identification System Magnification aperture distance, mm marking PL 4х/0.10 ∞/– 4х 0.10 19.8 – PL 10х/0.25 ∞/– 10х 0.25 – yellow PL FL 10х/0.35 ∞/– 10х 0.35 – yellow PL 20х/0.40 ∞/0.17 20х... -
Page 14: Reflected Light Illuminator
REF LECTE D LIGH T IL LU MI NATO R The general view of the reflected light illuminator is given in Fig. 3. Fig. 3. Reflected light illuminator 1. Illuminator body 5. Filter slider 8. Lamphouse locking screw 2. Turret wheel 6. -
Page 15: Camera
The excitation spectrum range: 320–550nm. The emission spectrum range: 435–700nm. The specifications of the reflected light illuminator (Table 3): Filter type LED excitation wavelength, nm Dichroic mirror wavelength, nm Emission wavelength, nm Ultraviolet (UV) 320–380 Violet (V) 380–415 Blue (B) 410–490 Green (G) 475–550... -
Page 16: Unpacking And Assembling The Microscope
3 UNPACKING AND ASSEMBLING THE MICROSCOPE The assembly procedure is given in Fig. 4. Fig. 4. Assembling the microscope 1. Remove the microscope from the package. 2. Check the scope of delivery using Section 8 of the User Manual. 3. Inspect the microscope and its components for damage. 4. -
Page 17: Brightfield Observations In Transmitted Light
4 BRIGHTFIELD OBSERVATIONS IN TRANSMITTED LIGHT SWITCHING ON T H E I LLU MI N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage. If not, do not switch on the microscope. Improper input voltage may result in a short circuit or fire. Set the filter turret wheel 2 (Fig. -
Page 18: Adjusting The Eyepiece Tubes
Looking into the right eyepiece (with the left eye closed) and lowering the stage slowly, bring the image into sharp focus using the coarse 2 and fine 1 focusing knobs. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use. If you need to adjust the tension of the coarse focusing knob, rotate the coarse focusing tension adjusting knob 4. -
Page 19: Setting Up Köhler Illumination
The adjustment range is ±5 diopters. The number on the ring corresponds to the diopter adjustment of the eyes. The indicator on the side is used for marking. We recommend memorizing your diopter adjustment value for future reference. Adjust the distance between the eyepieces to your interpupillary distance by rotating the eyepiece tubes around the central axis until you see a single circular image when looking through the eyepieces with both eyes. -
Page 20: Using Oil Immersion Objectives
– Open the field diaphragm 3 until it just disappears outside of the field of view. Additional centering may be required. – Remove the eyepiece from the right tube with no diopter adjustment and, while observing the objective exit pupil, open the aperture diaphragm to 2/3 of the objective exit pupil. -
Page 21: Calculating The Total Magnification
CALCU LATI N G T HE TOTAL M AGN IF I CAT I ON The total magnification is the eyepiece power multiplied by the objective power. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.85, the total magnification of the microscope is 10 х... -
Page 22: Fluorescence Observations
– Set the beam splitter lever 4 to the eyepiece position. While observing the specimen through the eyepieces, find a distinctive point in the field of view (an easily identifiable target, such as point S in Fig. 12а), move the specimen on the stage so that the point is in the center of the field of view, as shown in Fig. -
Page 23: Using The Reflected Light Illuminator
The light source is selected to match the filter type. The letters on the filter turret and the lamphouse turret must be the same when in operation. USI N G THE R EF L ECT ED L IGH T IL LUM IN ATOR 1. -
Page 24: Using Optional Equipment
Use the "shutter" position when you have completed the observations or during a break in order to avoid excessive excitation light exposure and prevent the specimen from fluorescence fading. Use the "neutral matt density" position in case of prolonged light exposure or under intense illumination of the specimen, which may distort the specimen configuration and affect the image quality and microscopy results. -
Page 25: Darkfield Slider
– Place a drop of immersion oil on the coverslip, place the 100х objective into the optical path and focus on the specimen. You should see the darkfield effect in the field of view (brightly shining particles of the specimen on a dark background). -
Page 26: Using The Eyepiece With A Scale
USI N G THE E YEP IECE WIT H A SCAL E The eyepiece with a scale or reticle can be used to make comparative analysis of the linear dimensions of the individual components of an object. The scale is installed in the plane of the field diaphragm of the 10x eyepiece. -
Page 27: Troubleshooting
7 TROUBLESHOOTING Potential problems and remedies (Table 4): Problem Cause Remedy ELECTRI CAL COMP ONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in the field of view The circuit board connector has poor Have the connector repaired by a qualified contact electronics technician... -
Page 28: Mechanical Components
MECHANI CAL C OMPONENTS The image does not remain The coarse focusing tension adjusting Adjust the coarse focusing tension adjusting sharp during observation knob is loosened, causing the stage knob to lower spontaneously The coarse focusing knob The coarse tension adjusting knob Loosen the tension of the coarse is too tight to rotate is overtightened... - Page 29 UV protective glass (shield) Filter slider In the reflected light illuminator Black protective plate for under-stage installation C-mount camera adapter Monitor Optional Calibration slide Optional ACCESSORIES AND SPARE PARTS Allen head screw for the reflected light illuminator Installed in the stand socket Head locking screw Installed in the illuminator socket Installed in the illuminator...
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Page 30: Care And Maintenance
9 CARE AND MAINTENANCE RE PL ACING T HE FUS E 1. Replacing the fuse in the microscope stand (for the transmitted light illuminator). Before replacing the fuse, turn the ON/OFF switch to "0" position (off). Unplug the power cord from the AC outlet and remove it from the connector on the back panel of the stand. -
Page 31: Magus Warranty
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 32 www.magusmicro.com...
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