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Levenhuk MAGUS BIO VD350 User Manual

Biological inverted digital microscope

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M AG US B IO V D3 5 0
BI OLOG I CA L I N VE RT ED D I G I TA L MI C RO S C OP E
U S E R M A N U A L

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Summary of Contents for Levenhuk MAGUS BIO VD350

  • Page 1 M AG US B IO V D3 5 0 BI OLOG I CA L I N VE RT ED D I G I TA L MI C RO S C OP E U S E R M A N U A L...
  • Page 2 Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
  • Page 3 12. Bulb installation. – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves or wrap the bulb with a cotton cloth. – Use a clean cotton cloth moistened with alcohol-based disinfectant to wipe dirt off the surface of the bulb.
  • Page 4 CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 MICROSCOPE PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Lamp house Stage Camera 3 UNPACKING AND ASSEMBLING THE MICROSCOPE 4 BRIGHTFIELD OBSERVATION PROCEDURE Switching on the illumination Using the beam splitter lever Adjusting the height and position of the condenser Adjusting the eyepiece tubes...
  • Page 5 MAGUS Bio VD350 Biological Inverted Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
  • Page 6 Coaxial coarse & fine focusing knobs on both sides Focusing mechanism  Fine focusing scale value: 2μm Coarse focusing lock knob and coarse focusing tension adjusting knob Light source 12V/30W halogen bulb with adjustable brightness Power supply AC voltage 85–265V, 50/60Hz, fuse specifications: 250V, 3.0A Phase-contrast turret condenser with an open slot and phase annuli for 10х, 20х...
  • Page 7 M ICROS COPE K IT This microscope kit includes the following main components: – stand with a built-in power supply, transmitted light source, focusing mechanism, fixed stage, revolving nosepiece, condenser mount, and trinocular tube. – trinocular microscope head – condenser –...
  • Page 8 Fig. 1. MAGUS Bio VD350 Microscope. View from the left 1. Eyepieces 8. Side camera port 2. Eyepiece tubes 9. Revolving nosepiece 3. Stand 10. Condenser mount 4. ON/OFF switch 11. Lamp house 5. Coarse focusing tension adjusting knob 12. Condenser 13.
  • Page 9 Fig. 2. MAGUS Bio VD350 Microscope. View from the right 1. Interpupillary distance adjustment ring 9. Object moving knob 2. Eyepiece tube assembly locking screw 10. Objectives 11. Condenser moving knob 3. Knob for switching the light path to the trinocular tube 12.
  • Page 10 MICROSCOPE PARTS STAND The stand has stable ergonomic design. Parts attached to the microscope stand 3 (Fig. 1): – revolving nosepiece 9 (Fig. 1) with objectives – stage 18 (Fig. 2) – condenser mount 10 (Fig. 1) – lamp house 11 (Fig. 1) –...
  • Page 11 The interpupillary distance is adjusted by rotating the eyepiece tubes 2 (Fig. 1) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale 1 (Fig. 2). For convenience, the microscope head is inclined at 45°. Microscope head magnification: 1x.
  • Page 12 LA M P HOUS E The microscope lamp house allows for setting up Köhler illumination. The lamp house 11 (Fig. 1) is fixed on the microscope stand. The light source – 12V/30W halogen bulb – is adjusted to the optical path and collector lens using three screws 14, 15, 16 (Fig. 2). A collector with a field diaphragm, which is adjusted to match the magnification of the objective positioned in the optical path, is fixed in the lamp house.
  • Page 13 UNPACKING AND ASSEMBLING THE MICROSCOPE The assembly procedure is given in Fig. 3. Fig. 3. Assembling the microscope 1. Unpack the microscope and check the scope of delivery using Section 8 of the User Manual. 2. Take out the stand 1 and place it on a stable work table, remove protective packaging and the dust cover. 3.
  • Page 14 BRIGHTFIELD OBSERVATION PROCEDURE SWITCHING ON T H E I LLU MI N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage. If not, do not switch on the microscope. Improper input voltage may result in a short circuit or fire. Set the ON/OFF switch 1 to "-"...
  • Page 15 ADJ U STI N G THE E YE P I ECE T UB ES Rotate the diopter adjustment ring 1 to find the position, at which the height of both eyepieces will be the same. Adjust the interpupillary distance. Adjust the distance between the eyepieces to your interpupillary distance by rotating the eyepiece tubes around the central axis until you see a single circular image when looking through the eyepieces with both...
  • Page 16 P LACI NG THE SP EC I ME N Choose the appropriate dish holder from the kit based on its shape and size (Fig. 11). Install the dish holder on the stage. Fix the slide 1 or the dish with the studied sample in it. Fig.
  • Page 17 When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob. Adjust the coarse focusing tension. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use.
  • Page 18 O BSE RVI N G SP EC IME N S IN TAL L GLAS SWA R E The microscope is equipped with a stage attachment 1 that enables movement of the specimen in longitudinal (Y) and lateral (X) directions. The object is moved by Y-axis 2 and X-axis 3 coaxial knobs, as shown in Fig.
  • Page 19 CALCU LATI N G T HE TOTAL M AGN IF I CAT I ON The total magnification is the eyepiece power multiplied by the objective power. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the total magnification of the microscope is 10х40 = 400х.
  • Page 20 – Place the 10х objective into the optical path. Looking through the eyepieces, bring the specimen into sharp focus. – Switch on the camera as described in the camera’s user manual. – Pull the beam splitter lever 3. If the image on the screen is blurry, adjust the focus using the fine focusing knob. When choosing the camera port, keep in mind that you will view upright images in the main (trinocular) port.
  • Page 21 You should use a special stage micrometer (calibration slide) to determine the linear dimensions (in millimeters or microns). The calibration slide is a transparent glass (of the same size as the specimen 1 DI V. = 0 . 0 1 MM slide) that has a micrometer scale with a scale division of 0.01mm etched on the surface.
  • Page 22 7 TROUBLESHOOTING Potential problems and remedies (Table 3): Problem Cause Remedy ELECT RI CAL C OMPONENTS The ON/OFF switch is off Switch on the ON/OFF switch The halogen bulb is damaged Replace the halogen bulb The fuse has blown Replace the fuse No illumination in the field of view Poor electrical contact...
  • Page 23 MECHANI CAL C OMPONENTS The image does not remain The coarse focusing tension adjusting Adjust the coarse focusing tension sharp during observation knob is loosened, causing the stage adjusting knob to lower spontaneously The coarse focusing knob is The coarse tension adjusting knob Loosen the tension of the coarse too tight to rotate is overtightened...
  • Page 24 ACCESSORIES AND SPARE PARTS Head locking screw Installed in the stand socket Allen wrench Dust cap Supplied 12V/30W halogen bulb In the lamp house Color filter Optional Fuse One in the lamp house AC Power cord Dust cover User manual D IG ITA L CA ME R A Digital camera USB cable...
  • Page 25 MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
  • Page 26 www.magusmicro.com...

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