Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for Levenhuk MAGUS LUM 400
Summary of Contents for Levenhuk MAGUS LUM 400
- Page 1 M AG US LUM 40 0 F LU O R ESC EN C E MI CRO S C O P E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241119...
-
Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 4 11. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 12. Bulb installation: – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves or wrap the bulb with a cotton cloth.
-
Page 5: Table Of Contents
CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 MICROSCOPE PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage Epi-fluorescence attachment 3 UNPACKING AND ASSEMBLING THE MICROSCOPE 4 BRIGHTFIELD OBSERVATIONS IN TRANSMITTED LIGHT Switching on the illumination Placing the specimen Focusing on the specimen Adjusting the eyepiece tubes... -
Page 6: Description Of The Microscope
MAGUS Lum 400 Fluorescence Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. -
Page 7: Microscope Kit
Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing lock knob Coarse focusing tension adjusting knob Transmitted light source 12V/30W halogen bulb with adjustable brightness Reflected light source 100W mercury lamp AC voltage 85–265V, 50/60Hz... - Page 8 Fig. 1. MAGUS Lum 400 Fluorescence Microscope. View from the right 1. Eyepieces 9. Transmitted light brightness 16. Filter slider (shutter/neutral adjustment ring density filter/free slot) 2. Eyepiece tubes 10. Coarse focusing tension 17. Field aperture centering knob of 3. Epi-fluorescence illuminator...
- Page 9 11 10 Fig. 2. MAGUS Lum 400 Fluorescence Microscope. View from the left 1. Diopter adjustment ring 8. Stand and base 15. Field diaphragm 21. Mercury lamphouse centering knob of the epi- power cord 2. Revolving nosepiece 9. Condenser locking screw fluorescence illuminator 22.
-
Page 10: Microscope Parts
MICROSCOPE PARTS STAN D The stand 8 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 2 (Fig. 2) with objectives 3 (Fig. 2) – stage 4 (Fig. 2) –... -
Page 11: Eyepieces
The interpupillary distance is adjusted by rotating the eyepiece tubes 2 (Fig. 1) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale. For convenience, the microscope head is inclined at 30°. Microscope head magnification: 1x. -
Page 12: Condenser
The specifications of the objectives (Table 2): Numerical Working Coverslip, Color Objective identification System Magnification aperture distance, mm marking PL 4х/0.10 ∞/– 4х 0.10 19.8 – PL 10х/0.25 ∞/– 10х 0.25 – yellow PL FL 10х/0.35 ∞/– 10х 0.35 – yellow PL 20х/0.40 ∞/0.17 20х... -
Page 13: Epi-Fluorescence Attachment
EP I- FLU ORESC E NC E AT TACH M EN T The general view of the epi-fluorescence attachment is given in Fig. 3. Fig. 3. Epi-fluorescence illuminator 1. Illuminator body 5. Field diaphragm centering knob 9. Mercury lamphouse of the epi-fluorescence illuminator 2. - Page 14 The turret wheel can be locked in one of the five positions corresponding to one of the four filters mounted on the turret: blue (B), green (G), violet (V), and ultraviolet (UV), or the neutral position (0). The marking of each filter corresponds to the color of the excitation light and its symbol is inscribed on the wheel 2 (Fig.
- Page 15 Fig. 4. Mercury lamphouse 1 . Lamphouse cover locking screw 6. Mercury lamp 2 . Lamphouse cover 7. Lamp installation rings 3, 4. Lamp adjustment knobs 8. Lamp locking screws 5 . Lamphouse power cord Centering the mercury lamp in the optical path is performed by the knobs 3 and 4 (Fig. 4). The knob 4 moves the lamp holder vertically, and the knob 3 moves it horizontally.
-
Page 16: Unpacking And Assembling The Microscope
The mercury lamphouse power cord is plugged into the connector of the power supply 7 (Fig. 5). The power supply is connected to the AC power outlet using an AC power cord. The AC power cord is plugged into the connector 4 (Fig. 5). The mercury lamp power supply is turned on by the switch 1 (Fig. -
Page 17: Brightfield Observations In Transmitted Light
Fig. 6b. Installing the mercury lamp 10. Connect the power cord of the mercury lamphouse to the power supply connector 8. Plug the AC power cord into the connector 9. Plug the power cord into an AC outlet. 11. Place the microscope head 3 on the epi-fluorescence illuminator 6. Secure the attachment screw using an Allen wrench. 12. -
Page 18: Placing The Specimen
PL AC IN G THE SP EC IM E N Place the specimen 1 on the stage. Adjust the image by moving the stage control knobs 2 and 3 so that the observed section of the specimen is directly under the objective. The stage attachment features an XY control system. -
Page 19: Adjusting The Eyepiece Tubes
ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale "0" to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus. -
Page 20: Setting Up Köhler Illumination
SE TTI N G U P KÖ H L ER IL LU MI NAT IO N In the light optical microscope, the image quality depends equally on the optics and on the illumination system, so adjusting the illumination is an important preparatory step. The illumination system affects the image resolution, comfort during long observation, and photo quality when using digital cameras. -
Page 21: Using Oil Immersion Objectives
When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field. -
Page 22: Fluorescence Observations
5 FLUORESCENCE OBSERVATIONS Before starting fluorescence observations, adjust the microscope to work in transmitted light using the brightfield technique, as described above. Placing the specimen, focusing on the specimen and adjusting the microscope head for the fluorescence observations are done in the same way as for the transmitted light observations. During fluorescence observations: –... - Page 23 Fig. 13. Switching on the epi-fluorescence illuminator 3. Using the filter slider The filter slider 9 has three positions: neutral matt density filter, free slot, and shutter. Once inserted in the illuminator slot, the plate is locked in one of the three positions. Use the "shutter"...
- Page 24 5. Centering the mercury lamp The optical fluorescence system is pre-centered at the factory. Since the mercury lamp is packaged separately, the center of the mercury lamp may deviate from the light path when it is re-installed. You should center the lamp before operation.
-
Page 25: Using Optional Equipment
6 USING OPTIONAL EQUIPMENT DARK F IEL D CO N D E N SER The optional darkfield condenser is used in the darkfield microscopy technique. This technique is used to obtain the image of unstained transparent weakly absorbing samples and therefore invisible when observed in the bright field. We recommend setting up the darkfield illumination with the oil condenser as follows: –... -
Page 26: Polarizer/Analyzer Set
PO L ARI ZE R/A N ALYZ E R S E T The polarization technique requires using the polarizer/analyzer set which consists of an analyzer and a polarizer. 1. Place the analyzer into the slot above the revolving nosepiece. 2. Place the polarizer on the collector. 3. -
Page 27: Using The Camera
USI N G THE CA ME RA The microscope is designed to observe a specimen through the eyepieces and to photograph the specimen. The trinocular tube is located on the top of the microscope head. When not in operation, it is covered with the dust cap 2. -
Page 28: Using The Calibration Slide With A Camera
U SING THE CAL I B R ATI O N SLI D E WI T H A CA M ER A The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification and indicate the known distance. -
Page 29: Mechanical Components
The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate coverslip thickness Use the specimen with the coverslip of standard thickness (0.17mm) The specimen is mounted upside down Place the specimen with the coverslip facing up There is immersion oil on the front lens Remove immersion oil from the front lens of the dry objective (most often 40x). -
Page 30: Scope Of Delivery
8 SCOPE OF DELIVERY The scope of delivery (Table 5) Component Note MIC RO S C OP E MAIN COMPONENTS Stand (with the transmitted light illuminator, power source and focusing mechanism built into the base) Epi-fluorescence illuminator Mercury lamphouse ICO Infinitive trinocular microscope head Revolving nosepiece Mounted on the stand Stage... -
Page 31: Care And Maintenance
Fluorescence light source: 100W mercury lamp In the epi-fluorescence illuminator Color filter set for transmitted illumination 3A/250V Fuse Installed in the illumination system Installed in the mercury lamphouse 15A/250V Fuse power supply Bottle of immersion oil AC Power cord Mercury lamphouse power cord Installed on the lamphouse Dust cover User manual... -
Page 32: Replacing The Mercury Lamp And Fuse (For The Epi-Fluorescence Illuminator)
REPLACING THE MERCURY LAMP AND FUSE (FOR THE EPI-FLUORESCENCE ILLUMINATOR) 1. Replacing the mercury lamp Switch off the mercury lamphouse power supply. Unplug the power cord from the power outlet. Wait approximately 30 minutes for the mercury lamp and lamphouse to cool down. –... -
Page 33: Maintenance
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 34 www.magusmicro.com...
Need help?
Do you have a question about the MAGUS LUM 400 and is the answer not in the manual?
Questions and answers