Related Manuals for Levenhuk MAGUS LUM D400 LCD
Summary of Contents for Levenhuk MAGUS LUM D400 LCD
- Page 1 M AG US LUM D 4 00 LC D FLU OR ES CE N C E D I G ITA L M I C ROS C O P E USER M ANUAL...
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Page 2: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 3 13. The fluorescence light source is a mercury lamp. The design of the mercury lamphouse does not allow light rays from the lamp to reach the eyes of the observer and others. To avoid burns, do not touch the surfaces of the mercury lamphouse during operation and for 15 minutes after the lamp has been switched off.
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Page 4: Table Of Contents
CONTENTS D ES CR I PT I O N OF T H E MICROSC OPE Purpose Specifications Microscope kit 2 M I C ROS C O PE PA RTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage Reflected light illuminator Camera Monitor... -
Page 5: D Es Cr I Pt I O N Of T H E Microsc Ope
MAGUS Lum D400 LCD Fluorescence Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. - Page 6 Abbe condenser (N.A. 1.2). Centerable. With adjustable aperture diaphragm Condenser Height-adjustable. Dovetail mount Field diaphragm Adjustable iris Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing lock knob Coarse focusing tension adjusting knob Transmitted light source...
- Page 7 Frame rate, fps at resolution, pix 30@3840х2160 (HDMI), 30@1920х1080 (Wi-Fi), 30@3840х2160 (USB 3.0) Image format *.jpg, *.tif Video format *.h264/*.h265, *.mp4 Spectral range, nm 380–650 (IR-filtered) Shutter type ERS (electronic rolling shutter) Windows 8/10/11 (32 and 64 bit), Mac OS X, Linux, up to 2.8 GHz Intel Core 2 or System requirements higher, minimum 4GB RAM, USB 2.0 ports, RJ45, CD-ROM, 19"...
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Page 8: Microscope Kit
Package dimensions (WxHxD) 302mm×960mm×452mm Weight without package 12.8kg Weight with package 17.3kg * Not included in the kit, available on request. ** The magnification of the microscope can be increased by using additional (optional) eyepieces and objectives. The manufacturer reserves the right to make changes to the product range and specifications without prior notice. M ICROS COPE K IT The microscope kit includes the following main components: –... - Page 9 Fig. 1. MAGUS Lum D400 LCD. View from the right 1. Eyepieces 10. Coarse focusing tension 17. Field aperture centering knob of adjusting ring the reflected light illuminator 2. Eyepiece tubes 11. Coarse focusing knob 18. Mercury lamphouse 3. Reflected light illuminator 12.
- Page 10 11 10 Fig. 2. MAGUS Lum D400 LCD. View from the left 1. Diopter adjustment ring 9. Condenser locking screw 15. Field aperture centering 21. Mercury lamphouse knob of the reflected power cord 2. Revolving nosepiece 10. Coarse focusing light illuminator lock knob 22.
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Page 11: I Crosc O P E Pa Rts
M I CROSC O P E PA RTS STAND The stand 8 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 2 (Fig. 2) with objectives 3 (Fig. 2) –... -
Page 12: Eyepieces
Microscope head magnification: 1x. Eyepiece diameter: 30mm. The eyepiece diopter adjustment is intended to compensate for the observer's ametropia. It is located on the left eyepiece tube – ring 1 (Fig. 2). The second eyepiece tube is fixed. The Gemel head design allows for the 360° rotation of the tubes, adjusting the eyepoint height for the convenience of users of different heights. -
Page 13: Condenser
The specifications of the objectives are given in Table 2. Table 2 Numerical Working Coverslip, Цветовая Objective identification System Magnification aperture distance, mm маркировка PL 4х/0.10 ∞ /– 4х 0.10 19.8 – PL 10х/0.25 ∞ /– 10х 0.25 – yellow PL FL 10х/0.35 ∞/–... - Page 14 Fig. 3. Reflected light illuminator 1. Illuminator body 5. Field aperture centering knob of 9. Mercury lamphouse the reflected light illuminator 2. Turret wheel 10. Collector adjustment knob 6. Filter slider 3. UV shield 11. Diaphragm adjustment knob of 7. Lamphouse locking screw the reflected light illuminator 4.
- Page 15 The excitation spectrum range: 320–555nm. The emission spectrum range: 435–700nm. The microscope employs broadband filters. The excitation occurs in a selected wavelength range, and the emission filter transmits the entire spectrum with a wavelength longer than the specified wavelength. The G position (Green) means that the fluorescence filter introduced in the optical path emits monochromatic radiation of the green spectrum range of 495–555nm from the overall radiation of the light source (250–900nm).
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Page 16: Camera
Attention: do not touch the glass surface of the mercury lamp! Centering the mercury lamp in the optical path is performed by the knobs 3 and 4 (Fig. 4). The knob 4 moves the lamp holder vertically, and the knob 3 moves it horizontally. There is a power cord attached to the lamphouse cover. -
Page 17: N Pac K I Ng A N D Asse M Blin G T H E Mic Ros C Op E
U NPACK I N G A ND AS S E M BL IN G T HE M IC ROS C OP E The assembly procedure is given in Fig. 6. Fig. 6. Assembling the microscope 1. Remove the microscope from the package. 2. -
Page 18: 4 B R I G H T Field Ob S Ervat Io N S In T Ra N Sm It T Ed L I Ght
Use the screwdriver to loosen the screws 1. Open the cover 2. Loosen two screws 5 on the lamp socket 6 and remove the plastic rod. Remove the mercury lamp 4 from the package and insert its ends into the socket rings. Position the center of the lamp in line with the two center screws and Phillips screw 7. -
Page 19: Focusing On The Specimen
The knob 2 controls Y-axis movement, the knob 3 controls X-axis movement. Moving range: 70mm in X-axis direction and 50mm in Y-axis direction. Fig. 8. Placing the specimen FOCU S IN G ON T H E S PECI ME N Place the 4x objective into the optical path (we recommend starting with low and medium magnification objectives that have a sufficiently large field of view and working distance). -
Page 20: Adjusting The Eyepiece Tubes
ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale "0" to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus. -
Page 21: Setting Up Köhler Illumination
We recommend memorizing your interpupillary distance for future reference. The eyepiece tubes are 360° rotatable to change the eyepoint height for the users of different heights. With an interpupillary distance being 64mm, a 180° rotation changes the eyepoint height by 50mm. S ETT IN G U P KÖ... -
Page 22: Using Oil Immersion Objectives
When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field. - Page 23 1. Dust cap screw 2. Dust cap 3. Trinocular tube centering screw 4. Beam splitter lever 5. Camera Fig. 13. Using the camera To enable the camera: – Loosen the screw 1. Remove the dust cap 2 from the trinocular tube. –...
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Page 24: Using The Monitor
U SIN G THE MO NI TO R The IPS matrix delivers a bright image with large viewing angles, which allows you to look at the display even at an angle with no color distortion. To display the image on the screen: –... -
Page 25: Using The Reflected Light Illuminator
– In order to prevent the fluorescence from quenching, the specimens should be placed in the optical path 5–10 minutes after switching on the lamphouse power supply, when the mercury lamp becomes fully operational. – To extend the life of the mercury lamp, do not switch the power supply on and off frequently. If you need to switch it off, wait about 15 minutes after ignition. - Page 26 3. Using the filter slider The filter slider 9 has three positions: neutral matt density filter, free slot, and shutter. Once inserted in the illuminator slot, the plate is locked in one of the three positions. Use the "shutter" position when you have completed the observations or during a break in order to avoid excessive excitation light exposure and prevent the specimen from fluorescence fading.
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Page 27: 6 U S I N G Opt Io Na L Eq U Ip M En T
Re-install the removed objective into the revolving nosepiece. The microscope is prepared for the fluorescence observations. You can move the filter slider into a "neutral matt density" position, place the specimen on the stage, focus the microscope and start observations. Fig. -
Page 28: Darkfield Slider
For good darkfield performance, specimens with a slide thickness of no more than 1.2mm and a coverslip thickness of no more than 0.17mm should be used. When using the darkfield technique with the immersion objective having a high aperture, the objective captures not only the light scattered by the specimen particles, but also the direct rays that create a light background and deteriorate the image contrast. -
Page 29: Using The Eyepiece With A Scale
U SIN G THE EY EP I ECE WI T H A SCAL E The eyepiece with a scale or reticle can be used to make comparative analysis of the linear dimensions of the individual components of an object. The scale is installed in the plane of the field diaphragm of the 10x eyepiece. The eyepiece with a scale is installed in the tube in place of the eyepiece of your microscope. -
Page 30: Troubleshooting
6. Enter the calibration setting and check the result. The program will save the calibration factor. 7. You can select any measurement unit later, and all the results will be re-calculated in accordance with this selection. TROUBLESHOOTING Potential problems and remedies are given in Table 4. Table 4 Problem Cause... -
Page 31: Mechanical Components
The focal plane of The specimen does not lie flat on the Place the specimen flat on the stage, securing it with the the image is tilted stage specimen holder (brighter on one side and darker on the other) Fluorescence is not The wrong fluorescence filter has been Select the filter to match the selected fluorescent dye observed... -
Page 32: C O Pe Of D E Live Ry
S CO P E OF D EL IVE RY The scope of delivery is listed in Table 5. Table 5 Component Note MIC ROS C O P E MAIN C O MP ONEN TS Stand (with the transmitted light illuminator, power source and focusing mechanism built into the base) Reflected light illuminator Mercury lamphouse... -
Page 33: Digital Camera
Fluorescence light source: 100W mercury lamp In the lamphouse Color filter set for transmitted illumination 3A/250V Fuse Installed in the illumination system Installed in the mercury lamphouse 15A/250V Fuse power supply Bottle of immersion oil AC Power cord Mercury lamphouse power cord Installed on the lamphouse Dust cover User manual... -
Page 34: Replacing The Mercury Lamp And Fuse (For The Reflected Light Illuminator)
Unplug the power cord from the AC outlet and remove it from the connector on the back panel of the stand 2. Carefully lay the microscope on its side. Loosen the lock screw 4, open the cover of the lamp housing. Replace the halogen bulb 3 with a new one. -
Page 35: Maintenance
Replacing the fuse in the mercury lamphouse power supply. Unplug the AC power cord from the power outlet and from the mercury lamphouse power supply. The fuse holder is located on the back panel of the power supply above the AC input connector. Using a flathead screwdriver, hook and remove the fuse block from the fuse holder. -
Page 36: Ag Us War Ra N T Y
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 37 ww w.magusmic ro.co m...
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