Levenhuk MAGUS BIO 260T Manual

Biological microscope

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M AG US BIO 260T | D H 2 6 0

B I OLOG ICA L MI CRO S C OP E

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Page 1 M AG US BIO 260T | D H 2 6 0 B I OLOG ICA L MI CRO S C OP E...
Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241028...
Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
Page 4 11. Do not use any other oily substance instead of proper immersion oil made specifically for the given purpose, as this will degrade the image quality and damage the lenses. 12. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean.
Page 5: Table Of Contents
CONTENTS 1 DESCRIPTION OF THE MICROSCOPE Purpose Specifications Microscope kit 2 COMPONENTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage Illuminator Built-in digital camera (DH260) 3 UNPACKING AND ASSEMBLING 4 BRIGHTFIELD OBSERVATION PROCEDURE Switching on the illumination Adjusting the LCD screen functions Placing the specimen Focusing on the specimen...
Page 6: Description Of The Microscope
The microscope is used in biomedical laboratories, biotechnology, material science, pharmaceutical research, agriculture, environmental studies, and forensics. The microscope can be used for scientific purposes, laboratory diagnosis, and education. S P ECIFICATION S ( TAB LE 1 ) Microscope MAGUS Bio 260T MAGUS Bio DH260 Magnification, x 40–1000 (**40–1250/1500/2000) Tube length Infinity (∞)
Page 7 Camera – C-mount camera adapter 0.5х – Transmitted light source 3W LED Automatic brightness adjustment during objective change, Intelligent lighting control system status display on LCD screen, standby mode, eco mode LCD screen Wi-Fi – 10–40x phase-contrast slider 100x phase-contrast slider Phase-contrast device 1* Centering telescope Phase-contrast objective (10x, 20x, 40x, 100x)
Page 8: Microscope Kit
M ICROS COPE K IT The microscope kit includes the following main components: – stand with the transmitted light source, focusing mechanism, stage, condenser, and revolving nosepiece – microscope head – set of objectives and eyepieces – set of spare parts and accessories –...
Page 9 1. Eyepieces 5. Specimen holder 9. XY stage control knob 2. Eyepiece tubes 6. LCD-screen 10. Head locking screw 3. Revolving nosepiece 7. Fine focusing knob 11. Microscope head 4. Objectives 8. Coarse focusing knob 12. Trinocular tube (MAGUS Bio 260T)
Page 10 Fig. 2. MAGUS Bio 260 Biological Microscope. View from the left 1. Stage 5. Lighting control knob 9. Coarse focusing tension adjusting knob 2. Aperture diaphragm ring 6. ON/OFF switch 10. Condenser focus knob 3. Abbe condenser 7. Fine focusing knob 11.
Page 11 Fig. 3. MAGUS Bio 260 Biological Microscope. Rear view 1. Microscope head with built-in 2. Carrying handle 4. Power cord holder camera (MAGUS Bio DH260) 3. USB-port 5. Power connector...
Page 12: Components
COMPONENTS STAND The stand 11 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 3 (Fig. 1) with objectives 4 (Fig. 1) – stage 1 (Fig. 2) –...
Page 13: Eyepieces
The trinocular microscope head: A C-mount adapter is installed in the vertical port 12 (Fig. 1) to fix the camera. The camera is used to transmit the image to a computer screen or monitor/TV. The beam splitting ratio (eyepieces/ trinocular tube) is 50/50. The binocular microscope head with a built-in camera makes it more convenient to work with digital devices.
Page 14: Condenser
Plan APO 60х/1,42 oil oil immersion 60х 1.42 0.25 0.17 blue ∞ /0,17 Plan 100x/1,25 oil oil immersion 100x 1.25 0.17 white ∞/0,17 Plan 100x/1,10W water 100х 1.10 0.16 0.17 white ∞/0,17 immersion The 50x, 60x and 100x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object.
Page 15: Built-In Digital Camera (Dh260)
BUI LT-I N D I GITAL CAME RA ( D H 26 0 ) 1. Type-C port 2. USB-mouse port 3. HDMI port 4. LAN port Fig. 4. General view of the microscope head with a built-in camera HDMI mode Use an HDMI cable to connect the digital microscope head to an HDMI monitor 3.
Page 16: Unpacking And Assembling
3 UNPACKING AND ASSEMBLING The assembly procedure is given in Fig. 5. Fig. 5. Assembling the microscope 1. Remove the microscope from the package. 2. Check the scope of delivery using Section 7 of the User Manual. 3. Inspect the microscope and its components for damage. 4.
Page 17: Brightfield Observation Procedure
4 BRIGHTFIELD OBSERVATION PROCEDURE SWITCHING ON T H E I LLU MI N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage. If not, do not switch on the microscope.
Page 18 Sleep mode Press the knob once to enter the standby mode. SLEEP will appear on the screen. Press the knob again to exit the Sleep mode. Time setting for the Sleep mode There is an eco mode in the microscope: If you do not operate it for a certain time interval, it enters a standby mode.
Page 19: Placing The Specimen
P LACI NG THE SP EC I ME N Place the specimen 1 on the stage by gently pushing aside the clip of the specimen holder. Adjust the image by moving the stage control knobs 2 and 3 so that the observed section of the specimen is directly under the objective.
Page 20: Setting Up Köhler Illumination
Adjust the distance between the eyepieces to your interpupillary distance by rotating the eyepiece tubes around the central axis until you see a single circular image when looking through the eyepieces with both eyes. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale 1 on the microscope head.
Page 21: Using Oil Immersion Objectives
When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field.
Page 22: Using Optional Equipment
USING OPTIONAL EQUIPMENT DARK FI EL D SLID E R The darkfield slider is designed for the darkfield microscopy on objectives with apertures up to 0.9. The slider is a plate with two round openings. One opening is free for the brightfield technique. The second opening holds the darkfield diaphragm.
Page 23: Phase-Contrast Device
PHASE- CO NTR AST DE V I CE The phase-contrast device is designed for studying low-contrast objects which are invisible in transmitted light of the brightfield microscopy. The phase-contrast technique allows for the observation of unstained low-contrast objects, colorless transparent specimens and living microorganisms. For example, it is used in medicine to calculate the number of platelets in clinical blood tests, visualize and count red blood cells in urine.
Page 24: Using The Camera
We recommend entering the obtained data in a size chart: Objective magnification Eyepiece division value Using these data to determine the actual linear size of the specimen, you just need to count the number of divisions of the eyepiece scale aligned with the area of the specimen being measured, and multiply this number by the scale division value specified in this table.
Page 25: Troubleshooting
3. Get a contrast image of the scale on the monitor screen and capture the image. 4. Select the ‘Calibrate’ function in the software you are using. 5. Double-click on the maximum visible distance and enter the value in actual units. 6.
Page 26 The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate coverslip thickness Use the specimen with the coverslip of standard thickness (0.17mm) The specimen is mounted upside Place the specimen with the coverslip down facing up There is immersion oil on the front Remove immersion oil from the front lens lens of the dry objective (most often...
Page 27: Scope Of Delivery
SCOPE OF DELIVERY The scope of delivery (Table 4) Component Note 260T DH260 MAIN COMPONENTS Stand (with focusing mechanism and LCD screen) Trinocular microscope head Binocular microscope head with built-in camera Revolving nosepiece Mounted on the stand Stage Mounted on the stand Transmitted light LED illuminator Mounted on the stand REPLACEABLE PARTS...
Page 28: Care And Maintenance
PlanF S-Apo 10x plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm PlanF S-Apo 20x plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm PlanF S-Apo 40x plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm PlanF 100x (oil) plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm Eyecups...
Page 29: Maintenance
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
Page 30 www.magusmicro.com...

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