Shodex UB Series Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex UB series is size exclusion chromatography columns
used with aqueous solvents. The column series is suitable for separating bionanoproducts such as viruses,
virus-like particles (VLPs), and exosomes. Please select columns with target molecular sizes (see below) that
meet the molecular size of your samples.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F6429301 UB-50
F6429302 UB-100
Base Material : Spherical porous particles of polyhydroxymethacrylate
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
4. Usable Conditions
Product Name
Recommended
UB-50
0.5 - 1.0
UB-100
Operation Manual
Shodex UB series
Column Size (mm)
I.D.
8.0
8.0
Flow Rate (mL/min)
Maximum
2.0
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
300 26
300 27
Maximum Pressure
(MPa/column)
0.5
ver.20241120E
Theoretical Target Molecular
Plate Number Size
(Per Column) (nm)
≥ 3,500 < 50
≥ 3,500 > 50
* Estimated value
Temperature
pH Range
Range (°C)
2 - 14 4 - 60
*
1