Shodex PROTEIN LW-803 Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.

Before Using the Column

(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.

1. Introduction

Thank you for purchasing the Shodex product. Shodex PROTEIN LW-803 is a silica-based gel filtration
chromatography column. The column is packed with particles having carefully optimized pore-sizes. It is suitable
for analyzing proteins with a molecular weight of several hundred of thousand.

2. Column Components

Please refer to the Shodex website: https://www.shodex.com/en/da/07.html

3. Column Specifications

Product
Product Name
Code
PROTEIN LW-803
F6989303
PROTEIN LW-G 6B
F6700133
Base Material : Spherical porous particles of silica modified with hydrophilic group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water

4. Usable Conditions

Product Name
PROTEIN LW-803
PROTEIN LW-G 6B
Operation Manual
Shodex PROTEIN LW-803
Column Size
Particle
(mm)
Size
(μm)
I.D. Length
8.0 300 3
6.0 50 3
Flow Rate (mL/min)
Recommended Maximum
1.0
-
Resonac Corporation (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 12,000 10,000 - 700,000
(Guard Column)
Reference value only / Measured with proteins
*1
Maximum
Pressure
(MPa/column)
1.5 15
- -
ver.20221202E
Exclusion
*1 Limit *1
(1,000,000) *2
- -
*2 Estimated value
pH Temperature
Range Range (°C)
3.0 - 7.5 4 - 45
1