Shodex GPC HK-400 Series Operation Manual

（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex GPC HK-400 series is an ultra-rapid analysis type
size exclusion chromatography column used with organic solvents. The column series is suitable for the
separations of organic compounds and/or oligomers, and molecular weight distribution analysis of high
molecular weight polymers. The columns with different exclusion limits are available. Please select columns
with target molecular weight ranges (see below) that meet the molecular weight range of your samples.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6025010 GPC HK-401
F6025020 GPC HK-402
F6025030 GPC HK-403
F6026040 GPC HK-404L
F6025050 GPC HK-405
F6025060 GPC HK-406
HK-404L is a mixed-gel column, suitable for the analysis of wide molecular weight ranges.
Operation Manual

Shodex GPC HK-400 series
Column Size
Particle
(mm)
Size
(μm)
I.D. Length
4.6 150 3
4.6 150 3
4.6 150 3.5
4.6 150 3.5
4.6 150 3
4.6 150 6.5
SHOWA DENKO K.K. (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 9,000 100 - 1,500
≥ 12,000 200 - 10,000
≥ 9,000 2,000 - 70,000
≥ 9,000 100 - 1,000,000
≥ 7,000 10,000 - 2,500,000
≥ 5,000 30,000 - 8,000,000
*
Reference value only / Measured with polystyrene
ver.211004E
Exclusion
* Limit *
2,000
20,000
100,000
1,000,000
4,000,000
10,000,000
1