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- Page 1 Instructions For Use TLA-55 Fixed-Angle Rotor For Use in the Beckman Coulter Optima MAX-XP, MAX-TL, MAX, MAX-E, TL, TLX, and TL-100 Tabletop Ultracentrifuges TL-TB-020AJ October 2022 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 • In the UK, call us at +44 845 600 1345 • In Ireland, call us at +353 (01) 4073082 • In Italy, call us at +39 0295392 456 • In other locales, contact your local Beckman Coulter Representative. Find us on the World Wide Web at: www.beckman.com...
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Page 3: Revision History
Revision History When a subsequent version affects the information in this document, a new issue will be released to the Beckman Coulter website. For updates, go to www.beckman.com/techdocs and download the latest version of the manual or system help for your instrument. - Page 4 Revision History TL-TB-020AJ...
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Page 5: Safety Notice
Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval. -
Page 6: Alerts For Warning, Caution, Important, And Note
This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval. - Page 7 Beckman Coulter Representative or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated.
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Page 8: Radiation Safety
Observe all warnings and cautions listed for any external devices attached or used during operation of the instrument. Refer to applicable external device user’s manuals for operating procedures of that device. NOTE For Safety Data Sheets (SDS) information, go to the Beckman Coulter website at https:// www.beckman.com/techdocs. WARNING Risk of chemical injury from bleach. - Page 9 Safety Notice Chemical and Biological Safety WARNING Risk of personal injury or property damage. The rotors and accessories are not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials in, nor handle or store them near the centrifuge.
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Page 10: Mechanical Safety
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage. -
Page 11: Maintenance
It is your responsibility to decontaminate components of the instrument before requesting service by a Beckman Coulter Representative or returning parts to Beckman Coulter. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated. - Page 12 Safety Notice Disposal TL-TB-020AJ...
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Page 13: Table Of Contents
Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, Important, and Note, vi Safety Information for the TLA-55 Rotor, vi Safety During Installation and/or Maintenance, vii Radiation Safety, viii Chemical and Biological Safety, viii Mechanical Safety, x Cleaning, x... - Page 14 Contents Replacement Rotor Parts, 15 Other, 15 Beckman Coulter, Inc. Ultracentrifuge Rotor Warranty Related Documents...
- Page 15 Illustrations Illustrations Arranging Tubes in the Rotor, 4 Precipitation Curves for the TLA-55 Rotor, 10 CsCl Gradients at Equilibrium for the TLA-55 Rotor, 11...
- Page 16 Tables Tables Relative Centrifugal Fields for the TLA-55 Rotor, 8...
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Page 17: Fixed-Angle Rotor
TLA-55 Fixed-Angle Rotor Specifications Maximum speed 55,000 RPM 45° Density rating at maximum speed 1.7 g/mL Relative Centrifugal Field at maximum speed at r (55 mm) 186,000 g at r (40 mm) 136,000 g at r (25 mm) 84,700 ... -
Page 18: Description
4. Fluid-Containment Annulus 5. Lid O-ring (inner) (824412) This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. The TLA-55 fixed-angle rotor holds up to twelve 1.5-mL microcentrifuge tubes at a 45-degree angle. -
Page 19: Preparation And Use
Preparation and Use Preparation and Use Specific information about the TLA-55 rotor is given here. Information common to this and other rotors is contained in Rotors and Tubes for Tabletop Preparative Ultracentrifuges (publication TLR-IM), which should be used together with this manual for complete rotor and accessory operation. Publication TLR-IM is included in the literature package shipped with the rotor. -
Page 20: Operation
TLA-55 Fixed-Angle Rotor Preparation and Use Figure 1 Arranging Tubes in the Rotor NOTE Two, three, four, six, eight, nine, ten, or twelve tubes can be centrifuged per run if they are arranged in the rotor as shown. Operation Use an absorbent towel to wipe off condensation from the rotor, then carefully place the rotor on the drive hub. -
Page 21: Removal And Sample Recovery
TLA-55 Fixed-Angle Rotor Preparation and Use Refer to the instrument instruction manual for ultracentrifuge operation. For additional operating information, see the following: Times, page 6, for using k factors to adjust run durations. • Speeds, page 7, for information about speed limitations. -
Page 22: Tubes
Run Times The k factor of the rotor is a measure of the rotor’s pelleting efficiency. Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes. The k factor is calculated from the formula: EQ 1 ⁄... -
Page 23: Run Speeds
TLA-55 Fixed-Angle Rotor Preparation and Use Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows: EQ 4 55,000 --------------------------------------- - actual run speed Run times can also be estimated from data established in prior experiments if the k factor of the previous rotor is known. - Page 24 TLA-55 Fixed-Angle Rotor Preparation and Use Table 1 Relative Centrifugal Fields for the TLA-55 Rotor Rotor Speed Relative Centrifugal Field ( g) k Factor* (RPM) At r At r At r (55 mm) (40 mm) (25 mm) 55,000 186,000 136,000...
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Page 25: Selecting Cscl Gradients
Each curve in Figure 3 is within the density limits allowed for the TLA-55 rotor: each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities (one for each fill level) that avoid precipitation at that speed. - Page 26 TLA-55 Fixed-Angle Rotor Selecting CsCl Gradients Figure 2 Precipitation Curves for the TLA-55 Rotor 1.90 1.85 1.80 1.75 1.70 TLA-55 ROTOR = 20°C 1.65 = 4°C 1.60 Rotor Speed (K RPM) * Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation.
- Page 27 TLA-55 Fixed-Angle Rotor Selecting CsCl Gradients Figure 3 CsCl Gradients at Equilibrium for the TLA-55 Rotor 1.90 1.85 1.80 1.75 1.70 1.65 1.60 1.55 TLA-55 ROTOR = 20°C 1.50 = 4°C 1.45 1.40 23.1 27.0 30.9 34.8 38.7 42.7 46.6 50.5...
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Page 28: Care And Maintenance
Replace O-rings about twice a year or whenever worn or damaged. Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. - Page 29 TLA-55 Fixed-Angle Rotor Care and Maintenance Wash the rotor and lid in a mild detergent, such as Beckman Solution 555 (339555), that won’t damage the rotor. • The Rotor Cleaning Kit contains two plastic-coated brushes and two quarts of Solution 555 for use with rotors and accessories.
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Page 30: Decontamination
Use the minimum immersion time for each solution, per laboratory standards. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 31: Supply List
Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. Contact Beckman Coulter Sales (1-800-742-2345 in the United States) or see the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (BR-8101, available at www.beckman.com) for detailed information on ordering parts and supplies. - Page 32 TLA-55 Fixed-Angle Rotor Supply List 1-16 TL-TB-020AJ...
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Page 33: Beckman Coulter, Inc
2. If a Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge (i) all centrifuge parts required for repair (except the drive... - Page 34 Beckman Coulter will repair or replace the rotor free of charge. 4. If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube, bottle, tube cap, spacer, or adapter, covered under the Conditions of this Warranty, Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point (2) above, and the replacement policy.
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Page 36: Related Documents
Related Documents Rotors and Tubes for Beckman Coulter Tabletop Preparative Ultracentrifuges (TLR-IM-9) • Rotors Tubes and Accessories • • Using Tubes and Accessories • Using Rotors Care and Maintenance • • Chemical Resistances • The Use of Cesium Chloride Curves Gradient Materials •...
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