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Summary of Contents for Endress+Hauser analytikjena qTOWER³
- Page 1 Operating Manual qTOWER³ / qTOWER³ G Real-Time PCR Thermal Cycler...
- Page 2 Manufacturer Analytik Jena GmbH Konrad-Zuse-Straße 1 07745 Jena / Germany Phone: +49 3641 77 70 Fax: +49 3641 77 9279 Mail: info@analytik-jena.com Technical Service Biometra GmbH Rudolf-Wissell-Straße 30 37079 Göttingen / Germany Phone: +49 551 50 68 60 Fax: + 49 551 50 68 666 Email: service@analytik-jena.com For a proper and safe use of this product follow the instructions.
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Page 3: Table Of Contents
qTOWER³ / qTOWER³ G Table of contents Table of contents 1 Notes on this operating manual ............................ 2 Intended use..................................3 Safety instructions ................................Safety markings ..............................Requirements for the operating personnel..................... Safety instructions – transport and installation....................Safety instructions – operation........................3.4.1 General safety instructions for operation......................3.4.2 Safety instructions –... - Page 4 Table of contents qTOWER³ / qTOWER³ G 9.1.3 Moving the device in the laboratory........................ 33 Storage ................................34 10 Disposal ................................... 35 11 Specifications .................................. 36 11.1 Technical data..............................36 11.2 Ambient conditions ............................38 11.3 Standards and directives ..........................38...
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Page 5: Notes On This Operating Manual
qTOWER³ / qTOWER³ G Notes on this operating manual Notes on this operating manual This operating manual describes the following thermocycler models: ¡ qTOWER³ qTOWER³ G ¡ In the text below, these devices are collectively called qTOWER³ , or simply ' d evice' . Dif- ferences between the models are explained in the corresponding section. -
Page 6: Intended Use
Intended use qTOWER³ / qTOWER³ G Intended use NOTICE The device is intended for general laboratory use. Its use is limited to applications out- side of in-vitro diagnostics (research use only). The device may only be used for the applications described in these operating instruc- tions. - Page 7 qTOWER³ / qTOWER³ G Intended use LOGAN, Julie; EDWARDS, Kristin; SAUNDERS, Nick (ed.): Real-Time PCR – Current Tech- nology and Application. Norfolk UK: Caister Academic Press, 2009...
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Page 8: Safety Instructions
Safety instructions qTOWER³ / qTOWER³ G Safety instructions For your own safety and to ensure error-free and safe operation of the device, please read this chapter carefully before commissioning. Observe all safety instructions listed in this user manual and all messages and displayed by the control and analysis software on the monitor. -
Page 9: Safety Instructions - Transport And Installation
qTOWER³ / qTOWER³ G Safety instructions When using the device, observe the appropriate laboratory diligence and cleanliness ¡ to prevent contamination of the device. This reduces the risk of the user being con- taminated with potentially infectious material and the risk of cross-contamination of the samples. -
Page 10: Safety Instructions - Protection Against Explosion And Fire
Safety instructions qTOWER³ / qTOWER³ G Do not use any oil between the samples and the sample block! The use of oil during ¡ device operation can result in the formation of health-hazardous vapors. Using oil to ensure better heat exchange is not necessary due to the excellent heat conductance of the installed sample block. -
Page 11: Handling Of Auxiliary And Operating Materials And Samples
qTOWER³ / qTOWER³ G Safety instructions 3.4.4 Handling of auxiliary and operating materials and samples The operator is responsible for the selection of substances used in the process, as well as for their safe handling. This applies in particular for radioactive, pathogenic, infectious, poisonous, corrosive, flammable, explosive or otherwise hazardous materials. -
Page 12: Design And Function
Design and function qTOWER³ / qTOWER³ G Design and function Design, connections and control elements The device combines a PCR thermocycler with a patented fluorescence photometer. The lock with handle and the LED for status display are located on the front side of the device. -
Page 13: Figure 3 Back
qTOWER³ / qTOWER³ G Design and function The mains connection and the power switch are located on the device rear. The interface for connection to the PC is also located on the device rear. Figure 3 Back 1 Ventilation for fluorescence spectrome- 2 Ventilation grille on the thermal cycler 3 Service connection 4 Power connection 5 Fuse holder for device fuses... -
Page 14: Function
Design and function qTOWER³ / qTOWER³ G Figure 5 Mains and connection cable Only use the mains cable supplied or a mains cable with the same specifications. Function 4.2.1 Fluorescence spectrometer The detector unit for a thermal block with 96 wells is a patented 8-channel epi-fluores- cence photometer with fiber multiplexer and a mechanical scanning device. -
Page 15: Figure 7 Schematic Representation Of The Light Beam Path Through A Filter Color Module
qTOWER³ / qTOWER³ G Design and function Measuring head The fluorescent dyes are specifically excited in the reaction mix thanks to the light and emit light of a longer wavelength. Using the lenses in the shuttle system, the emitted light is bundled and passed back to the color modules via the optical fibers. Photomultiplier (PMT) In the color modules, the light passes the beam divider followed by two emission filters and is then transferred to the photomultiplier (CPM) for detection. -
Page 16: Pcr Thermocycler
Design and function qTOWER³ / qTOWER³ G 4.2.2 PCR thermocycler The thermal block with 96 wells is made of silver for the best possible performance and thermal conductivity. The silver is coated with gold for corrosion protection. Due to its excellent heat conductivity, silver equilibrates extremely quickly, providing maximum speed and uniform temperature distribution. -
Page 17: Type Plate
qTOWER³ / qTOWER³ G Design and function Type plate The type plate is located on the rear of the device. You can find the following informa- tion displayed in the image there: Figure 8 Type plate 1 Manufacturer and address 2 Protection class of the housing 3 Safety symbol (Attention! Observe the 4 Device number accompanying documentation!) -
Page 18: Installation
Installation qTOWER³ / qTOWER³ G Installation Installation location requirements Ambient conditions The climate conditions for the installation location are listed in the technical specifica- tions (→ "Ambient conditions" 38). If required, ensure that the room is tempera- ture-controlled. Installation location require- This laboratory device is designed for indoor use. ¡... -
Page 19: Installation
qTOWER³ / qTOWER³ G Installation Electrical connection require- Operating voltage 100 / 115 / 230 V (AC) ments Line frequency 50 to 60 Hz Power consumption ≤ 850 W Device fuse 2 x 10 AT / 250 V Installation WARNING Risk of electric shock! Check that the mains connection conditions match those indicated on the type plate on the rear of the device. Before connecting the device to the mains network set the correct operating voltage at the voltage switch on the underside of the device. -
Page 20: Figure 9 Voltage Selection Switch On The Bottom Of The Device
Installation qTOWER³ / qTOWER³ G Figure 9 Voltage selection switch on the bottom of the device } Open the lid of the device. To do so, press in the red handle on the front until the lock clicks open. Fold back the upper part of the device. } Remove the transport lock and put it aside for later transports. -
Page 21: Operation
qTOWER³ / qTOWER³ G Operation Operation Switching the device on and off Switching on the device } Switch on the device via the mains switch on the rear of the device. ü The device is initialized. During this time, the status LED on the front side of the device flashes. -
Page 22: Starting A Real-Time Pcr Analysis
Operation qTOWER³ / qTOWER³ G Starting a real-time PCR analysis WARNING Biological hazard! Exercise caution when working with potentially infectious materials. Wear suitable pro- tective equipment, e.g. protective gloves. WARNING Risk of eye injury! The rapid heating of the thermal block can result in the explosive vaporization of liquids when the thermal block is opened during the PCR run. -
Page 23: Figure 11 Positions Of Additional 8-Well Strips When Measuring With Few Samples
qTOWER³ / qTOWER³ G Operation When using few well strips, place one strip on each side of the sample block. ¡ Figure 11Positions of additional 8-well strips when measuring with few samples Start a real-time PCR analysis as follows: } Pipette the PCR samples into the sample vessels. Close the sample vessels. NOTICE! Micro titer plates must be sealed with optically transparent adhesive foil (sealing foil). -
Page 24: Error Messages
Error messages qTOWER³ / qTOWER³ G Error messages The following chapter describes possible errors of the device. The error correction mea- sures that can be performed by the customer are limited to those listed in the following section. NOTICE If you cannot eliminate errors yourself, please contact customer service. If an error occurs, the software outputs error codes assigned to the following errors: Error code Cause... -
Page 25: Maintenance And Care
qTOWER³ / qTOWER³ G Maintenance and care Maintenance and care WARNING Risk of electric shock! Do not touch! Prior to commencing any maintenance or cleaning work, switch off the device and un- plug the power plug. The following maintenance and care tasks can be performed by the customer: ¡... -
Page 26: Disinfecting The Device
Maintenance and care qTOWER³ / qTOWER³ G Dust or reagent residue in the sample block can cause an increase in the background sig- nal. ¡ Use compressed air to blow out the recesses in the sample block. ¡ To remove reagent residue, fill the affected wells with max. 20 µL of distilled water or ethanol. -
Page 27: Replacing Fuses
qTOWER³ / qTOWER³ G Maintenance and care Replacing fuses WARNING Risk of electric shock! Do not touch! Before exchanging fuses, switch off the power switch and disconnect the device from the mains network. Only use the specified fuses. If the wrong fuses are used, there is a risk of fire, injuries and device damage. - Page 28 Maintenance and care qTOWER³ / qTOWER³ G Installing color modules The proper installation of the color modules is as follows: } Switch off the device. } Open the lid. Remove the four external screws on the inside of the lid. } Close the lid. } Remove the four outer screws on the rear of the device on the upper part the hood is fastened with.
- Page 29 qTOWER³ / qTOWER³ G Maintenance and care } Note the code on the color module and the position in which the color module is to be installed. Remove the old color module or dummy ele- ment (dummy module). To do this, unscrew the two M2 screws and pull the color module out.
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Page 30: Figure 13 Selection Window For Color Modules. Note: With Software Version 4.1 And Higher, Only The Color Module
Maintenance and care qTOWER³ / qTOWER³ G Registering color modules in To use the color modules correctly in the software, these must be registered in the soft- the software ware. } Switch on the device. Start the software. } Select menu item Extras | Color modules. The Edit color modules window opens. - Page 31 qTOWER³ / qTOWER³ G Maintenance and care Changing color module proper- The properties of a color module can be changed or re-specified. ties } Mark the color module in the list. } Activate the Properties checkbox. } In the Position list, select the position of the color module on the carrier in the flourescence measuring head.
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Page 32: Transport And Storage
Transport and storage qTOWER³ / qTOWER³ G Transport and storage Transport NOTICE Use suitable packaging material and transport locks! Unsuitable packaging material may cause damage to the device! Only transport the de- vice in its original packaging and with the transport lock in place! Information on proper packaging is included with the device. -
Page 33: Return
qTOWER³ / qTOWER³ G Transport and storage 9.1.2 Return WARNING Risk of damage to health due to improper decontamination! Perform a professional and documented decontamination of the device before returning it to Analytik Jena. The decontamination report is available from the customer service department when registering the return. -
Page 34: Storage
Transport and storage qTOWER³ / qTOWER³ G Storage NOTICE Environmental influences and condensate formation can destroy individual components of the device! The device must only be stored in air-conditioned rooms. The atmosphere must be low in dust and free from aggressive vapors. If the device is not installed immediately after delivery or not required for prolonged pe- riods, it should be stored in its original packaging. -
Page 35: Disposal
qTOWER³ / qTOWER³ G Disposal Disposal The operator of the device must dispose the waste materials that occur during mea- surements (sample materials) in accordance with the statutory and local regulations. At the end of its service life, the device and all its electronic components must be dis- posed of as electronic waste in accordance with applicable regulations. -
Page 36: Specifications
Specifications qTOWER³ / qTOWER³ G Specifications 11.1 Technical data General data Dimensions (height x width x depth) 59 cm x 27.5 cm x 27.5 cm, 70 cm x 27.5 cm x 50 cm when opened Mass 30 kg Noise level 45 dB Safety circuits Sensor-based monitoring of the closing ¡ and locking of the sample chamber Overtemperature protection in the ¡... - Page 37 qTOWER³ / qTOWER³ G Specifications Fluorescence spectrometer Measuring principle Fiber-optic shuttle system with 8-fold scan- ner and color modules for the excitation and emission filters Light source 4 long-life, high-performance LEDs (RGBW) Color modules ¡ 12 color-, FRET and protein modules ¡ 6 positions in the device Detector Highly sensitive PMT (photomultiplier tube) Color modules...
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Page 38: 11.2 Ambient Conditions
Specifications qTOWER³ / qTOWER³ G 11.2 Ambient conditions Operation Transport, storage Temperature range +15 °C to +35 °C +5 °C to +55 °C Max. humidity 70 % 10 % to 30 % Use desiccant! Max. permissible height 2000 m Air pressure 0.7 to 1.06 bar Work environment Only suitable for operation in rooms 11.3 Standards and directives Protection class and protection The device is protection class I. - Page 39 qTOWER³ / qTOWER³ G Table of figures Table of figures Figure 1 Front view................................12 Figure 2 Open device ................................12 Figure 3 Back..................................13 Figure 4 Voltage selection switch on the bottom of the device ..................13 Figure 5 Mains and connection cable ..........................14 Figure 6 Schematic representation of the fluorescence spectrometer ................
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