Related Manuals for ThermoFisher Scientific GeneChip 645i
Summary of Contents for ThermoFisher Scientific GeneChip 645i
- Page 1 GeneChip Hybridization Oven 645i ™ USER GUIDE Catalog Numbers 00-0331 Publication Number MAN0027670 Revision F For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Affymetrix Inc. Products: GeneChip Hybridization Oven 645i ™ 3450 Central Expressway Santa Clara, CA 95051 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.
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Page 3: Table Of Contents
Contents CHAPTER 1 The Hybridization Oven 645i ..... . . 4 Introduction ............. . . 4 Quick reference guide . -
Page 4: Chapter 1 The Hybridization Oven 645I
The Hybridization Oven 645i Introduction The Applied Biosystems GeneChip Hybridization Oven 645i (Figure 1) is a general ™ purpose laboratory oven with a rotating rotisserie designed for hybridization of Applied Biosystems GeneChip cartridge arrays. Electronic controls accurately ™ govern the oven temperature and provide visible status information to the operator. Figure 1 The GeneChip Hybridization Oven 645i. -
Page 5: Quick Reference Guide
Chapter 1 The Hybridization Oven 645i Quick reference guide Quick reference guide The hybridization oven is very simple to operate (Table Table 1 Quick reference guide for the Hybridization Oven 645i If you want to: Then do this: Description Turn on/off power. 1. -
Page 6: Safety Information
Chapter 1 The Hybridization Oven 645i Safety information Safety information These instructions contain important operating and safety information. You must carefully read and understand these instructions before using the hybridization oven. CAUTION! If you use this instrument in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired and the warranty may be voided. -
Page 7: Unpacking And Setup
Chapter 1 The Hybridization Oven 645i Unpacking and setup • Do not continue to use the oven if the temperature or rotisserie controllers fail, as evidenced by errors E01 through E12 being displayed (see the section, "Error codes and troubleshooting" on page 21). -
Page 8: Unpacking The Oven
Chapter 1 The Hybridization Oven 645i Unpacking and setup Locating the oven • Use a strong, level table or laboratory bench capable of supporting the weight of the oven. The oven weigh approximately 67 pounds (30 kg). • Provide an adequate source of electrical power within reach of the power cord. The oven operates on AC voltages from 100 to 240 volts nominal, 50/60 Hz, and draws currents below 5 Amperes. -
Page 9: Operating The Door
Chapter 1 The Hybridization Oven 645i Unpacking and setup Rear panel items The following items are located on the rear panel (Table Table 2 Rear panel items. Item What it does How to use Power entry module / Plug mating end of power cord into this connector. -
Page 10: Using The Rotisserie
Chapter 1 The Hybridization Oven 645i Using the rotisserie Using the rotisserie Installing the Follow this procedure to install a rotisserie into the oven. rotisserie into the oven 1. Set the power switch off and open the oven door. 2. Remove the rotisserie from the accessory box. Note that one end is equipped with a rubber “foot”... - Page 11 Chapter 1 The Hybridization Oven 645i Using the rotisserie Figure 4 Installing the rotisserie into the left socket. 5. Mount the right axle of the rotisserie into the right holder. Place the geared end of the axle into the right-side drive socket (Figure 5).
- Page 12 Chapter 1 The Hybridization Oven 645i Using the rotisserie Figure 6 The rotisserie installed in the oven. Electrical power supply and voltage selection • etermine the electrical voltage and style of appropriate grounded line cord for your location. • On the rear panel of the oven, locate the red 115/230 voltage selector switch (Figure Figure 7 The red 115/230 voltage selector.
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Page 13: Operating The Oven
Chapter 1 The Hybridization Oven 645i Operating the oven • After correct voltage and power cord are selected, remove the label covering the power entry receptacle and connect the power cord to the oven and an electrical power outlet (Figure Figure 8 The 3-pronged plug power receptacle. - Page 14 Chapter 1 The Hybridization Oven 645i Operating the oven System status The front panel of the oven contains the system status indicator (Figure 10). Yellow indicators indicates that the set point has not yet reached the set value. Green indicates that the set point has been reached and that the oven is operating normally.
- Page 15 Chapter 1 The Hybridization Oven 645i Operating the oven Applying power 1. Verify that the rotisserie is installed and the door is closed. and programming 2. On the left side of the front panel, locate the recessed power switch (the I/O switch the oven as shown in Table 1...
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Page 16: Loading The Rotisserie
Chapter 1 The Hybridization Oven 645i Operating the oven Figure 12 The temperature °C display and UP/DOWN keys. 8. Note that the oven may take about 30 minutes to reach the set point temperature. 9. If power is turned off, when restarted the oven remembers the temperature set point and rpm rate. - Page 17 Chapter 1 The Hybridization Oven 645i Operating the oven Figure 13 Installing the carrier retaining clips at the center of the crossbars. 6. Slide in a cartridge carrier. Only one direction is possible. (Figure 14) Figure 14 Slide the carrier into the rotisserie. 7.
- Page 18 Chapter 1 The Hybridization Oven 645i Operating the oven Figure 15 Clip the carrier retainer over the crossbars and the carrier. 8. Snap tight the carrier retainer on to the carrier (Figure 16). Figure 16 Snap tight the carrier retainer on to the carrier. 9.
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Page 19: Unloading The Rotisserie
Chapter 1 The Hybridization Oven 645i Operating the oven Figure 17 Installed cartridge carriers with the retaining clips set on the carriers. Note that the carriers are placed 180° apart to balance the rotisserie. 10. Close the oven door, set the temperature and set the rotisserie speed if needed. 11. -
Page 20: Communicating With The Oven
Chapter 1 The Hybridization Oven 645i Communicating with the oven Communicating with the oven Serial The Hybridization Oven 645i is equipped with a 9 pin connector RS-232 serial port, communication located on the rear panel. One 5 meter cable is provided in the oven accessory kit. Tx, Rx and GND are the active pins employed by the oven. - Page 21 Chapter 1 The Hybridization Oven 645i Communicating with the oven Error codes and Note that the oven contains no user repairable parts. Only qualified field service troubleshooting engineers should open any panels on the oven. Do not attempt to repair the oven yourself.
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Page 22: Oven Maintenance
Chapter 1 The Hybridization Oven 645i Oven maintenance Oven maintenance Cleaning All of the oven chamber walls are stainless steel to retard corrosion. NOTE: Remove saline solution spills with a damp cloth. Clean the interior of the oven regularly to keep the stainless steel from corroding. The exterior of the unit is painted metal and plastic. - Page 23 Chapter 1 The Hybridization Oven 645i Oven maintenance 3. Using a screwdriver and fingers, gently pry out and slide the fuse holder out of the module (Figure 19). Figure 19 Removing the fuse holder 4. Replace the defective fuses. 5. Slide the fuse holder back into the module until it snaps in place (Figure 20).
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Page 24: Genechip™ Hybridization Oven 645I Specifications
Chapter 1 The Hybridization Oven 645i GeneChip™ Hybridization Oven 645i specifications GeneChip Hybridization Oven 645i specifications ™ Applied Biosystems GeneChip Hybridization Oven 645i ™ Applied Biosystems Part Number 00-0331 Manufactured by Thermo Fisher Scientific, Model 308-1CEAFFY The GeneChip Hybridization Oven 645i is a General Purpose Laboratory Instrument. ™... -
Page 25: Regulatory Requirements
Chapter 1 The Hybridization Oven 645i Regulatory requirements Regulatory requirements The oven conforms to the following regulatory standards, or applicable equivalents, for the United States, Canada, and the European Union: • Product Safety for “Electrical Equipment for Measurement, Control, and Laboratory Use,”... -
Page 26: China Rohs Restriction Of Hazardous Substances Compliance
Chapter 1 The Hybridization Oven 645i China RoHS Restriction of Hazardous Substances compliance China RoHS Restriction of Hazardous Substances compliance Manufacturers of Electronic Information products (EIPs) that are sold to the People’s Republic of China, are required to provide information about lead, cadmium, mercury, hexavalent chromium, polybrominated biphenyls and polybrominated diphenyl ethers contained within. -
Page 27: China Rohs Restriction Of Hazardous Substances Compliance
Chapter 1 The Hybridization Oven 645i China RoHS Restriction of Hazardous Substances compliance China RoHS Restriction of Hazardous Substances compliance GeneChip Hybridization Oven 645i User Guide ™... -
Page 28: Customer And Technical Support
Support Customer and technical support Visit thermofisher.com/support for the latest in services and support, including: • Worldwide contact telephone numbers • Product support, including: – Product FAQs – Software, patches, and updates • Order and web support • Product documentation, including: –... - Page 29 For support visit thermofisher.com/support or email techsupport@lifetech.com thermofisher.com 4 May 2023...
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