ThermoFisher Scientific thermoscientific Cryofuge 5500i Instruction Manual
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ThermoFisher Scientific thermoscientific Cryofuge 5500i Instruction Manual

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Thermo Scientific
Cryofuge 5500i
Instruction Manual
20057781-f • 07 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

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Summary of Contents for ThermoFisher Scientific thermoscientific Cryofuge 5500i

  • Page 1 Thermo Scientific Cryofuge 5500i Instruction Manual 20057781-f • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...

  • Page 3: Table Of Contents

    Table of Contents Preface ............................iii Scope of Supply ........................iii Intended Use ..........................iv Accident Prevention .........................iv Precautions ..........................iv Chapter 1 Introduction and Description ....................1-1 Characteristics of the Cryofuge 5500i ..............1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ..................1-4 Functions and Features ......................
  • Page 4 Contents Chapter 5 Maintenance and Care ......................5-1 Cleaning Intervals ....................5-2 Cleaning ........................5-2 Cleaning the Venting Slots ..................5-3 Disinfection ......................5-3 Decontamination ..................... 5-4 Service of Thermo Fisher Scientific ................5-5 Chapter 6 Troubleshooting ........................6-1 Mechanical Emergency Door Release ..............6-2 Troubleshooting by User ..................

  • Page 5: Preface

    Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller's warranty.

  • Page 6: Intended Use

    Intended Use Intended Use • This centrifuge is a laboratory product designed to separate components by generation of a relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives. •...
  • Page 7 Precautions • Plug the centrifuge only into sockets which have been properly grounded. • Do not move the centrifuge while it is running. • Do not lean on the centrifuge. • Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific.
  • Page 8 Precautions Centrifuging hazardous substances: • Do not centrifuge explosive or flammable materials or substances which could react violently with one another. • The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. • Do not centrifuge inflammable substances. Remaining risk: Improper use can cause damages, contamination, and injuries with fatal consequences.
  • Page 9 Introduction and Description Contents • “Characteristics of the Cryofuge 5500i” page 1-2 “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-4 • • “Mains Supply” page 1-5 “Refrigerants” page 1-6 • “Rotor Selection” page 1-6 •...

  • Page 10: Chapter 1 Introduction And Description

    Introduction and Description Characteristics of the Cryofuge 5500i Characteristics of the Cryofuge 5500i The Cryofuge 5500i is a large volume, refrigerated universal centrifuge. The Cryofuge 5500i is designed for large volume, high throughput laboratory needs. The micro test plates and tubes can be centrifuged with the unique double rectangular bucket. The Cryofuge 5500i is optimized for the automatic laboratory processes in clinical central labs.

  • Page 11: Technical Data

    Introduction and Description Technical Data Technical Data The technical data of the Cryofuge 5500i is listed in the following table. Table 1-1. Technical Data Feature Value 230 V 200 V 120 V Environmental Conditions -Use in interior spaces -Altitudes of up to 2,000 m above sea level -max.

  • Page 12: Directives, Standards And Guidelines

    Introduction and Description Directives, Standards and Guidelines Directives, Standards and Guidelines Table 1-2. Directives, Standards and Guidelines Region Directive Standards and Guidelines Europe 2006/42/EC Machinery EN 61010-1 Protective goals of: EN 61010-2-020 2014/35/EU Low Voltage EN 61326-1 2014/30/EU Electromagnetic Compatibility (EMC) 2011/65/EU RoHS Directive on the Restriction of the use of certain Hazardous...

  • Page 13: Mains Supply

    Introduction and Description Mains Supply Function Feature Program Display Free Program Selection Operating Memory 1...9 Memory Capacity for Set Talues PULSE-Mode Acceleration / Braking Profiles 1 = slow, ... 9 = fast Acceleration / Deceleration Curve Speed Selection Adjustable from 300 rpm to 10,000 rpm in 10 rpm Increments RCF Selection After actuating the RCF Switch Key, the RCF Value can be entered.

  • Page 14: Refrigerants

    Introduction and Description Refrigerants Refrigerants Table 1-5. Refrigerants Article No. Centrifuge Refrigerant Quantity Pressure Thermo Scientific Cryofuge 5500i R-134A 1 kg 21 bar 1430 1.43 t 75004471 Thermo Scientific Cryofuge 5500i R-134A 1 kg 21 bar 1430 1.43 t 75004475 Thermo Scientific Cryofuge 5500i R-134A 1 kg...

  • Page 15: Before Use

    Before Use Contents “Before Setting up” on page 2-2 • • “Setting up” page 2-2 “Aligning the Centrifuge” page 2-4 • “Transporting the Centrifuge” page 2-4 • “Location” page 2-4 • “Mains Connection” page 2-5 • “Adjust Voltage and Frequency” page 2-6 •...

  • Page 16: Before Setting Up

    Before Use Before Setting up Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately, if any damage is discovered. 2. Remove the packaging. 3. Check the order for completeness (see “Scope of Supply”...
  • Page 17 Before Use Setting up Lock nut Threaded rod Safety bracket Threaded nut Locking stabilizer 4. Screw the threaded rods with the threaded nuts as far up as possible. Otherwise they might get stuck when rolling the centrifuge off the pallet. 5.

  • Page 18: Aligning The Centrifuge

    Before Use Aligning the Centrifuge Aligning the Centrifuge 11. Switch on the centrifuge. 12. Open the centrifuge. 13. Put the enclosed water level on the drive spindle. 14. Use an open-end wrench to align the centrifuge and to raise and lower it. CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be damaged.

  • Page 19: Mains Connection

    Before Use Mains Connection • The centrifuge should not be exposed to heat and strong sunlight. WARNING UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight. • The set-up location must be well-ventilated at all times. Mains Connection Mains plug Figure 2-1.

  • Page 20: Adjust Voltage And Frequency

    Before Use Adjust Voltage and Frequency Adjust Voltage and Frequency It is necessary that you set the voltage and frequency during setup. 1. Pull out the power supply plug. CAUTION Remove the power supply plug in order to prevent damage to the centrifuge. 2.

  • Page 21: Control Panel

    Control Panel Contents “Control Panel” page 3-2 • • “Keys” page 3-2 Thermo Scientific Cryofuge 5500i...

  • Page 22: Keys

    Control Panel Control Panel Control Panel The control panel contains the keys and displays of the centrifuge (only the power switch is located on the right side of the device). All parameters can be selected and changed during operation. OPEN Key Switch Temperature Acceleration curve...

  • Page 23: Operation

    Operation Contents “Switch on Centrifuge” page 4-2 • • “Centrifuge Door” page 4-2 “Rotor Installation” page 4-3 • • “Entering Parameters” page 4-4 “Centrifugation” page 4-8 • • “Temperature Adaptation during Standstill” page 4-9 “Short-term Centrifugation” page 4-9 • • “Removing the Rotor”...

  • Page 24: Switch On Centrifuge

    Operation Switch on Centrifuge Switch on Centrifuge 1. Turn on the power switch on the right side of the device. The centrifuge performs a self-check of its software. Subsequently, the following readings will be displayed. The display shows the values from the last run. Centrifuge Door WARNING The performance of the gas springs might diminish after some time.

  • Page 25: Rotor Installation

    Operation Rotor Installation Rotor Installation The approved rotors for the Cryofuge 5500i are listed in section “Rotor Selection” page 1-6. Use only the rotors and accessories from this list in the centrifuge. CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge.

  • Page 26: Entering Parameters

    Operation Entering Parameters Entering Parameters Acceleration Curve The Cryofuge 5500i offers you a total of 9 acceleration and 9 braking curves with which samples and gradients can be centrifuged. After the centrifuge is turned on, the last running profile selected is shown. 1.
  • Page 27 Operation Entering Parameters Explanation of RCF Value The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices, since it is independent of the type of device.
  • Page 28 Operation Entering Parameters After 10 minutes, the minutes are given. Continuous Operation 1. Press the key below the field running time. 2. Press the key up or down until the following is shown: During continuous operation, the centrifuge will continue running until you stop it manually with key.
  • Page 29 Operation Entering Parameters Pre-warming or Pre-cooling the Centrifuge For setting the pre-temp value for the centrifuge proceed as follows: 1. Press the key and enter the desired value by pressing up or down. The display shows: 2. Press the key. The rotor will be pre-temped for 30 to 60 minutes.

  • Page 30: Centrifugation

    Operation Centrifugation Changing the Settings during a Run You can change the set values during a run. Press either the key below the display. The value that is being changed flashes. When the new value is stored the actual value is shown in the display.

  • Page 31: Temperature Adaptation During Standstill

    Operation Temperature Adaptation during Standstill Stopping the Centrifugation Program With preset Running Time Usually the run time is preset and you only have to wait until the centrifuge stops automatically when the preset time limit expires. As soon as the speed drops to zero, the message will appear in the display.

  • Page 32: Removing The Rotor

    Operation Aligning the Centrifuge Removing the Rotor To remove the rotor, proceed as follows: 1. Open the centrifuge door. 2. Thread the fastening tool in the centrifuge spindle counter-clockwise. Hold the rotor with the other hand into position. 3. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle.

  • Page 33: Key Switch

    Operation Key Switch Key Switch The key can lock some functions of the centrifuge. Key position Description All settings can be changed. The user can change and use all programs in the program memory. PULSE and the pre-temp function can be used. The program memory is protected against changes.
  • Page 35 Maintenance and Care Contents “Cleaning Intervals” page 5-2 • • “Cleaning” page 5-2 “Cleaning the Venting Slots” page 5-3 • • “Disinfection” page 5-3 “Decontamination” page 5-4 • • “Service of Thermo Fisher Scientific” page 5-5 Thermo Scientific Cryofuge 5500i...

  • Page 36: Chapter 5 Maintenance And Care

    Maintenance and Care Cleaning Intervals Cleaning Intervals For the sake of personal, environmental and material protection, it is your duty to clean and if necessary, disinfect the centrifuge on a regular basis. Maintenance Recommended Interval Daily or when polluted Clean Rotor Chamber Daily or when polluted Clean Rotor Daily or when polluted...

  • Page 37: Cleaning The Venting Slots

    Maintenance and Care Cleaning the Venting Slots 6. Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning. CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the drive shaft or the bearings of the centrifuge. Organic solvents break down the grease in the motor bearing.

  • Page 38: Decontamination

    Maintenance and Care Decontamination The rotor chamber should be treated preferably with a neutral disinfectant. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used.

  • Page 39: Service Of Thermo Fisher Scientific

    Maintenance and Care Service of Thermo Fisher Scientific 4. Release the rotor. 5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 6. Remove the centrifuge tubes and adapters and dispose of them or disinfect them. 7.

  • Page 41: Troubleshooting

    Troubleshooting Contents “Mechanical Emergency Door Release” page 6-2 • • “Troubleshooting by User” page 6-3 “When to contact a Service Technician” page 6-5 • Thermo Scientific Cryofuge 5500i...

  • Page 42: Mechanical Emergency Door Release

    Troubleshooting Mechanical Emergency Door Release Mechanical Emergency Door Release During a power failure, you will not be able to open the centrifuge door with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop.

  • Page 43: Troubleshooting By User

    Troubleshooting Troubleshooting by User Troubleshooting by User If problems occur other than those listed in this table, the authorized customer service representative must be contacted. Failure message Problem with centrifuge Possible causes and cures Display remains The drive stops. No mains connection. dark.
  • Page 44 Troubleshooting Troubleshooting by User Failure message Problem with centrifuge Possible causes and cures OPEN Display Centrifuge will not start. Centrifuge door not properly closed. Open the centrifuge door appears although and repeat locking procedure. door is closed. Over-temperature in the motor detected. Pull out the power supply plug.

  • Page 45: When To Contact A Service Technician

    Troubleshooting When to contact a Service Technician Failure message Problem with centrifuge Possible causes and cures E-12 The run does not start or the centrifuge runs Temperature measurement error down without being braked. Restart the centrifuge. If an error message appears again, inform a service technician. E-14 Centrifuge does not start or decelerates to No rotor present or rotor identification impossible.
  • Page 46 Troubleshooting When to contact a Service Technician NV-RAM-Version 2 Cycle counter Note The values given are only examples. 2. Afterwards the different programs are tested as well. Program test 3. Communicate the software version to the service technician. Cryofuge 5500i Thermo Scientific...
  • Page 47 Chemical Compatibility Chart CHEMICAL 2-mercaptoethanol S S U - S M S - S U S S U S S - S S S S U S S S S S S Acetaldehyde U U - M U U U M M - M S U - Acetone M S U U S U M S S U U S U S U U U S S U U S M M S U U...
  • Page 48 Chemical Compatibility Chart CHEMICAL Cesium Bromide M S S - S S S - S S S S S S S - S S S S S S M S S S Cesium Chloride M S S U S S S - S S S S S S S - S S S S S S M S S S Cesium Formate...
  • Page 49 Chemical Compatibility Chart CHEMICAL Hydrochloric Acid (conc.) U U U U - U U M - U M U U M U U U - U S U U U - Formaldehyde (40 %) M M M S S S S M S S S S M S S S U S S M S S S M S M U Glutaraldehyde S S S S - S S S S S S S -...
  • Page 50 Chemical Compatibility Chart CHEMICAL Lactic Acid (20 %) S S - M S M - S S S S S S S M S M S S - N-Butyl Alcohol S U - S M - U S M S S S S M M S M - N-Butyl Phthalate S S U - S S S -...
  • Page 51 Chemical Compatibility Chart CHEMICAL Sucrose, Alkaline M S S - S S S - S S S S S S U S S S S S S S S M S S S Sulfosalicylic Acid U U S S S S S - S S S U S S S - S S S - S S S U S S S...
  • Page 52 Chemical Compatibility Chart Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc. Suggest testing under actual conditions of use. Unsatisfactory, not recommended. Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use.
  • Page 53 Index Acceleration Curve .......... 4-4 Keys ..............3-2 Accident Prevention ........P-iv Aerosol-tight Rotors ........4-10 Autoclaving ............. 5-5 Location ............2-4 Braking Curve ..........4-4 Mains Connection ...........2-5 Bucket Selection ..........4-7 Mains Supply ...........1-5, 2-6 Maintenance ...........5-1 Care ..............5-1 Centrifugation ..........4-8 Operation ............4-1 Cleaning ............
  • Page 54 Index Stopping the Centrifugation Program ..... 4-9 Storage ............2-6 Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .........4-9 Transporting the Centrifuge ......2-4 Trouble Shooting ..........6-1 Cryofuge 5500i Thermo Scientific...
  • Page 55 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2000-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

This manual is also suitable for:

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