Table of Contents Introduction Introduction Inspection and Setup Installation and Operation Installation Tee Installation Operation Calibration and Calculations Calibration Calculating Calibration Coefficients Recommended Measurement Practices Minimizing Variation in Signal Temperature Considerations Maintenance and Warranty Visual Inspection and Cleaning Maintenance Check Using Standard Solutions Warranty Terms Warranty Service Out of Warranty Service...
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Equipment Specified as Electrical and Electronic Waste 998-2810 Rev. A Page 3...
In-Line Fluorometer provides a 4 - 20 mA output signal proportional to the fluorescence in the sample or source water. In a representative application, the Enviro-T In-Line Fluorometer can be used with a data collection system to monitor and control the level of algal biomass in industrial applications. The sample water’s fluorescence intensity is measured by passing the sample water, with chlorophyll- containing organisms, past the Enviro-T In-Line Fluorometer’s optical window.
Near devices that produce a strong electromagnetic field such as large generators It is recommended that the Enviro-T In-Line Fluorometer be installed in such a way that the flow is directed upward to expel any trapped air bubbles as air trapped on the optical window will influence signal and cause erratic readings (see section 4.0).
Upon excitation, the fluorophore emits a different wavelength of light (fluorescence) that will be detected by the fluorometer’s photodiode. After power (8 - 30 VDC) is applied to the Enviro-T In-Line Fluorometer allow 5 seconds for the LED to stabilize. After 5 seconds measurements can be taken continuously as current output (4 –...
Enviro-T In-Line Fluorometer 3. Calibration and Calculations 3.1 Calibration 1) Connect the Enviro-T In-Line Fluorometer to the mounting tee provided (see section 2.2, steps 1-6). 2) Connect the Enviro-T In-Line Fluorometer to the power supply and digital multimeter (see section 2.2, step 7).
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Commo 11) Make sure to log the reading (see Table 1 for example). 12) You can now install the Enviro-T In-Line Fluorometer in line with the system you intend to monitor following installation instructions provided (see section 2.1). Data collected upon completing these calibration steps can be put into a table similar to the table below to track future calibrations.
Enviro-T In-Line Fluorometer 3.2 Calculating Calibration Coefficients With the data collected you can find the coefficients required for calculating concentrations using the equation: y = mx + c (Equation 1) Where: y is the sample concentration, m is the slope of your equation, x is the sensor output in mA, c is the x intercept (offset) when y = 0.
Turner Designs’ Enviro-T In-Line Fluorometer has a flat-surfaced optical window that might trap air bubbles when positioned vertically. For this reason we recommend: 1) Installing the Enviro-T In-Line Fluorometer horizontally so that the mounting tee is in a vertical position.
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Additional information on how to apply these corrections is included in the Turner Designs Application Note: A Practical Guide to Flow Measurements.
1) Make a standard solution (solution of known concentration of the fluorophore being measured) that is less than the maximum concentration set for the Enviro-T In- Line Fluorometer. 2) Flow this solution past the optical window and record the mA reading.
Turner Designs warrants the Enviro-T In-Line Fluorometer and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions: Turner Designs is not responsible for replacing parts damaged by accident or neglect.
Repair service will be billed on a fixed price basis, plus any applicable duties and/or taxes. Shipment to Turner Designs should be prepaid. Your bill will include return shipment freight charges.
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Equipment Specified as Electrical and Electronic Waste 998-2810 Rev. A Page 15...
Appendix A: Fluorometer and Mounting Tee Specifications Parameter Specification Linearity (over dynamic range) 0.99 r MDL for Chlorophyll 0.03 µg/L Dynamic Range for Chlorophyll 0-100 µg/L Power Draw 0.96 W @ 12 VDC (1.2 W max.) Input Voltage 8 – 30 VDC Signal Output 4 –...
Appendix B: Wiring Guide Wire Color Function Connection Supply Voltage PSU – Positive Connection 8 – 30 VDC Supply Ground PSU – Ground Connection Black 0 VDC Signal out to data logger, Multimeter “A” Connection Orange “A”, 4 – 20 mA DC Signal out to data logger, Multimeter “Common”...
Appendix C: In Vivo Chlorophyll Measurements Turner Designs’ Enviro-T In-Line Fluorometer can be configured with chlorophyll optics to determine the relative abundance of algae in water samples. The instrument provides a 4 - 20 mA output signal proportional to the fluorescence from the sample or source water being measured.
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Make sure to log the reading (see Table C1 for example). Your Enviro-T has been adjusted to read relative algal concentration between 0- 100 µg/L and you can now install the Enviro-T In-Line Fluorometer in line with the system you intend to use for signal monitoring or control, following installation instructions provided (see section 2.1 of manual).
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5) If the readings vary by more than 5% of the initial value recorded for the 8 ppb Rhodamine Wt concentration, see section 5.1 for visual inspection and cleaning Maintenance checks are important and will ensure the Enviro-T In-Line Fluorometer is continuing to provide maximum performance and measurement reliability.
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