abi PRISM 377 User Manual page 31

Dna sequencer
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Problem
Signal too weak
377-96 Error
Messages
Message
A Valid 96 Lane Firmware
Image is Required!
EP Voltage Deviation Exceeds
Tolerance
Warning: Plate Out. Thermistor
P43/J43 Open/Short Circuit
Warning: Plate In. Thermistor
P44/J44 Open/Short Circuit
Warning: Possible Heater
Thermistor Open/Short Circuit
4-4 Troubleshooting
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Possible Cause
Multiple
Possible Cause
A non-96 collection software
has tried to establish
communications with a 377
instrument that has the 96-lane
option installed.
The EP voltage deviated
outside its tolerance range. The
instrument operation is paused.
Indicates one of the following:
Possible open or short
circuit exists with the
thermistor/cable connected
to J43 or J44.
Temperature of the plate in
an instrument with the
100k ohm thermistors is
21.9 °C or less.
Solution
Increase the CCD gain to
four:
a.
Open 377-96 Collection.
b.
In the Run window select the
Run module.
c.
Double-click the small
document icon next to the
Run Module pulldown menu.
d.
Change the CCD gain to
four.
Resuspend samples in less
volume (concentrate).
Solution
Install the 96-lane collection
software and firmware.
Call service.
One of the thermistors is not
functioning properly.
Schedule a service call, and
continue to operate the
instrument as usual.
This message may appear
when you launch data
collection software and start a
plate check, prerun, or run.

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