Setting Run Conditions - abi PRISM 377 User Manual

Setting Run Conditions

Selecting a Run
Use the following table to select the type of comb to use based on the
number of lanes you are running.
Mode
Note
lanes is selected.
Note
will be the same number of data collection points per lane, but there will be an
area of blank space to the left and right of the samples due to extra scan width.
However, the Neural Net Tracker has been trained using gels run according to
the default parameters. (For example: 48-lane gels run in XL mode and 36 lane
gels run in Full Scan mode.) Any deviation from the default is likely to confuse
the tracker resulting in mistracked lanes.
Setting Run
To set gel run conditions:
Conditions
Step
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The correct run mode is automatically chosen when the number of
No. of Lanes
24 Shark's-tooth
24 Square-tooth
32 Shark's-tooth
34 Square-tooth
36 Shark's-tooth
36 Square-tooth
48 Shark's-tooth
50 Square-tooth
64 Shark's-tooth
66 Square-tooth
96 Shark's-tooth
It is possible to run a gel of any number lanes in 96 Scan mode. There
Action
1 Open the 377-96 Collection software.
2 Prepare a sample sheet as described in the ABI P
Sequencer User's Manual.
IMPORTANT Preparing a sample sheet prior to the run is
required for optimal tracker operation.
Comb Run Mode
Full Scan
Full Scan
Full Scan
Full Scan
Full Scan
Full Scan
XL Scan
XL Scan
XL Scan
XL Scan
96 Scan
RISM
377 DNA
Gels 2-5