abi PRISM 377 User Manual page 12

Preparing a Gel To prepare a gel for a run:
Step
1
2
3
4
5
6
7
8
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Action
Let the gel polymerize for at least two hours.
Remove the clamps from the gel. Leave the casting comb in place
until you are ready to insert the comb.
Rinse the plate thoroughly with dH
IMPORTANT Carefully clean the read region of the gel.
Slide out the casting comb without bending it.
IMPORTANT Do not pry the casting comb.
Using a razor blade, scrape off all excess acrylamide from the glass
in the loading area.
Using a squirt bottle, rinse the loading area with dH
at lint-free tissue.
Using a syringe, add 1x TBE in to the loading area.
Note Adding TBE eases the insertion of the comb.
IMPORTANT Be very careful not to introduce bubbles. They
are very difficult to remove once the comb has been inserted.
If necessary, clean the shark's tooth comb with dH
tissue.
O and let dry.
2
O and dry with
2
O and a lint-free
2
Gels 2-3