abi PRISM 377 User Manual page 17

Suggested The following table lists the suggested load volumes and sample
resuspension volumes for sequencing.
Sequencing Load
Volumes
a. 5:1 Deionized formamide to 50 mg blue dextran/mL in 25 mM
EDTA
b. Loading 1.5 µL requires a syringe with a 0.2-mm tip to facilitate
loading at the bottom of the well.
! WARNING !
teratogen. It can cause birth defects. Wash thoroughly after handling
formamide. Wear appropriate protective eyewear, clothing, and gloves.
Obtain a copy of the MSDS from the manufacturer. Wash thoroughly after
handling formamide.
Suggested The following table lists the suggested load volumes for GeneScan.
GeneScan Load
Volumes
a. Loading 1.5 µL requires a syringe with a
0.2-mm tip to facilitate loading at the bottom
of the well.
Note
v. 2 User's Manual.
2-8 Gels
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Resuspension
No. of Wells Vol. (µL)
24/36 6–9
48 2–4
64 2–4
96 2–4
CHEMICAL HAZARD. Formamide is a known
Loading Vol.
No. of Wells (µL)
24/36 1.5
50 1.0–1.5
66 0.5–1.0
96 1.0–1.5
For more details, refer to the GeneScan Reference Guide and the LMS
Loading Vol.
a
(µL)
1.5
1.0–1.5
1.0–1.5
b
1.0–1.5
a