Troubleshooting - abi PRISM 377 User Manual

Troubleshooting

Gels
Problem
Leaking wells
Error: "Your
CCD offset is
too high. I will
reset it to zero."
Comb is
difficult to
insert
4-2 Troubleshooting
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Possible Cause Solution
Loose combs Sequencing: Electrophorese
immediately, then after each three
loads with the eight-channel loader.
GeneScan: Leaking wells are not
tolerated in GeneScan applications. If
a well leaks, it is best to run another
gel. At the very least, do not use the
wells around the leaking lane.
Bad clamps Be sure to use three "bulldog" clamps
(P/N 4305386) with 10–12 lbs.
clamping pressure.
Burrs or bent Remove the burrs or replace the
teeth on comb comb.
Bent, kinked, or Replace the spacers.
damaged
spacers
The CCD Reset the CCD offset value:
reading is below
a. Open 377-96 Collection.
zero during
b. In the Run window select the
calibration scan
Run module.
c. Double-click the small document
icon next to the Run Module
pulldown menu.
d. Change the CCD offset value to
zero.
e. Click Save as Default.
Using a different Be sure to use same comb for
comb loading that was used for casting.
Clamps are too Insert comb slowly. Fix any
tight misaligned teeth with a syringe
before they touch the gel.
Use looser clamps on future
gels.